feature_id int64 0 16.4k | description stringlengths 43 3.53k | summary stringlengths 10 1.2k | activation_pattern stringlengths 0 1.23k | category stringclasses 14
values | exemplar_protein_families stringlengths 0 2.38k | uniref90_frequency int64 1.4M 208M | uniref90_idf float32 0 5 | uniref90_max_activation float32 7.8 50.7 | top_100_uniref_ids listlengths 100 100 | top_swissprot_activations listlengths 20 20 | decoder_nearest_neighbors listlengths 10 10 | threshold float64 0 1.22 ⌀ |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
0 | Summary: Acidic, metal-coordinating catalytic segment for phosphate/diphosphate chemistry, firing primarily on the Nudix hydrolase signature (the Nudix box, E(x)7E(x)11EE) and secondarily on analogous E/D-rich motifs that bind Mg2+ and position pyrophosphate in related enzymes (e.g., IDI, PRPP/salvage enzymes) and DNA-... | Acidic, metal-coordinating catalytic segment for phosphate/diphosphate chemistry, firing primarily on the Nudix hydrolase signature (the Nudix box, E(x)7E(x)11EE) and secondarily on analogous E/D-rich motifs that bind Mg2+ and position pyrophosphate in related enzymes (e.g., IDI, PRPP/salvage enzymes) and DNA-processin... | Regional activation centered on short acidic stretches with strong residue-level peaks on catalytic Glu/Asp; in Nudix proteins the peak is typically near the N‑terminal Nudix box; spans range from single residues to a short loop/helix segment | Catalytic function | - Nudix hydrolases (MutT/NUDT1 8‑oxo‑dGTPase): activation on the conserved Nudix box that coordinates Mg2+ for diphosphatase activity
- NAD‑capped RNA hydrolase (NudC): activation on the Nudix box that binds the NAD cap’s pyrophosphate
- Viral mRNA decapping Nudix enzymes: activation on the Nudix signature that cleaves... | 4,628,379 | 3.805359 | 13.757813 | [
{
"activation": 13.7578125,
"uniref_id": "A0A1I5ZIT8"
},
{
"activation": 13.7109375,
"uniref_id": "UPI002F91017D"
},
{
"activation": 13.6796875,
"uniref_id": "A0A1G6BH86"
},
{
"activation": 13.640625,
"uniref_id": "A0A7W7P4P0"
},
{
"activation": 13.6328125,
"u... | [
{
"activation": 11.051897048950195,
"uniprot_id": "P45799"
},
{
"activation": 11.000073432922363,
"uniprot_id": "A4WRA6"
},
{
"activation": 10.992526054382324,
"uniprot_id": "D8KIT5"
},
{
"activation": 10.925203323364258,
"uniprot_id": "Q9UKK9"
},
{
"activation": ... | [
82,
334,
15309,
7546,
14504,
1546,
15772,
15444,
13061,
3365
] | 0.380352 |
1 | Summary: Predominantly extracellular/secreted and cell-surface proteins with exposed, flexible low-complexity segments in the mature region (often Ser/Thr/Gly-rich or proteoglycan-like), frequently cysteine-rich; with a minority of metazoan lipid-binding proteins (including FABPs) that can be cytosolic
Activation patte... | Predominantly extracellular/secreted and cell-surface proteins with exposed, flexible low-complexity segments in the mature region (often Ser/Thr/Gly-rich or proteoglycan-like), frequently cysteine-rich; with a minority of metazoan lipid-binding proteins (including FABPs) that can be cytosolic | Regional activation on extended contiguous stretches of exposed/disordered segments within the mature region—Gly/Thr-enriched repeats and Ser/Thr-rich O-glycosylated linkers when present, or flexible surface loops; weak in transmembranes and compact catalytic cores | Disorder | - Gram-positive adhesins and sortase-anchored cell-wall proteins: activation on Ser/Thr/Gly-rich repeat stalks between the signal peptide and LPXTG anchor
- Type IV pilins and fimbrial/adhesin subunits: activation on extracellular low-complexity linker or repeat segments
- Hemophores and iron-acquisition surface protei... | 6,834,559 | 3.415573 | 18.828125 | [
{
"activation": 18.828125,
"uniref_id": "A0A3M1ZTU0"
},
{
"activation": 18.328125,
"uniref_id": "A0A956C9C0"
},
{
"activation": 18.203125,
"uniref_id": "A0A6L3A521"
},
{
"activation": 18.03125,
"uniref_id": "A0A952W8I6"
},
{
"activation": 18.03125,
"uniref_id"... | [
{
"activation": 12.867857933044434,
"uniprot_id": "H8WEC0"
},
{
"activation": 11.957571029663086,
"uniprot_id": "P9WI69"
},
{
"activation": 9.26235580444336,
"uniprot_id": "O00834"
},
{
"activation": 7.776237487792969,
"uniprot_id": "P46483"
},
{
"activation": 7.5... | [
9518,
3158,
7101,
4124,
6534,
9879,
4894,
5095,
1267,
16078
] | 0.30678 |
2 | Summary: Glycine-rich, acidic (Asp/Glu) and often histidine-bearing flexible capping/loop segments at the mouths of active sites or ligand-binding clefts that bind/position anionic cofactors or substrates (phosphate- or carboxylate-bearing; often Mg2+-assisted) and act as lids/gates across diverse folds; the same acidi... | Glycine-rich, acidic (Asp/Glu) and often histidine-bearing flexible capping/loop segments at the mouths of active sites or ligand-binding clefts that bind/position anionic cofactors or substrates (phosphate- or carboxylate-bearing; often Mg2+-assisted) and act as lids/gates across diverse folds; the same acidic Gly-ric... | Regional activation over contiguous mid-sequence blocks (≈15–50 residues), peaking on Gly clusters adjacent to Asp/Glu or His–Asp pairs; often one or a few such segments per protein | Ligand-binding site | - One‑carbon/folate enzymes (glycine cleavage T protein, folate‑linked demethylases, GAR transformylase/GART): activation on THF-binding/capping loops with Gly clusters and acidic residues that cradle the folate phosphate/carboxylates.
- Purine biosynthesis AIRS/PurM and related enzymes: activation on β–α loops in the ... | 9,601,427 | 3.075654 | 22.734375 | [
{
"activation": 22.734375,
"uniref_id": "A0A4S2EWG0"
},
{
"activation": 22.703125,
"uniref_id": "UPI0024BC8F7B"
},
{
"activation": 22.671875,
"uniref_id": "A0A7X6UYK5"
},
{
"activation": 22.609375,
"uniref_id": "A0A9D2INP9"
},
{
"activation": 22.578125,
"unire... | [
{
"activation": 21.305940628051758,
"uniprot_id": "Q681Y3"
},
{
"activation": 20.359771728515625,
"uniprot_id": "Q4FP21"
},
{
"activation": 19.548770904541016,
"uniprot_id": "P48728"
},
{
"activation": 19.227611541748047,
"uniprot_id": "Q9UI17"
},
{
"activation": ... | [
6453,
353,
1933,
4121,
15196,
10049,
4215,
14972,
14024,
12232
] | 0.187933 |
3 | Summary: Acidic, Ser/Thr-rich low-complexity segments on the non-lumenal side of membrane proteins—juxtamembrane loops and terminal tails that function as flexible regulatory/assembly and trafficking regions, often carrying phosphorylation sites and acidic motifs; typically disordered or only modestly structured, thoug... | Acidic, Ser/Thr-rich low-complexity segments on the non-lumenal side of membrane proteins—juxtamembrane loops and terminal tails that function as flexible regulatory/assembly and trafficking regions, often carrying phosphorylation sites and acidic motifs; typically disordered or only modestly structured, though sometim... | Regional activation across long contiguous stretches (≈20–50 aa), frequently multiple per protein, typically immediately flanking a transmembrane helix; strongest at clusters of Asp/Glu interspersed with Ser/Thr and Gly/Pro; minimal activation within hydrophobic transmembranes. | Membrane-associated | Viral Vpu: cytoplasmic acidic, serine-rich disordered C-terminal tail with phosphoserines; Mitochondrial calcium uniporter (MCU): acidic, partly helical/coiled C-terminal region directly after TM2 implicated in oligomerization/regulation; NADPH oxidase/DUOX/RBOH: cytosolic N-terminal regulatory segments enriched for ac... | 26,165,785 | 2.073112 | 16.84375 | [
{
"activation": 16.84375,
"uniref_id": "UPI00194225C9"
},
{
"activation": 16.609375,
"uniref_id": "UPI0030DB5CEF"
},
{
"activation": 16.546875,
"uniref_id": "A0A1R4JNN4"
},
{
"activation": 16.46875,
"uniref_id": "UPI00048F70B9"
},
{
"activation": 16.34375,
"un... | [
{
"activation": 14.78911304473877,
"uniprot_id": "Q75007"
},
{
"activation": 14.782535552978516,
"uniprot_id": "P08806"
},
{
"activation": 14.743574142456055,
"uniprot_id": "P19554"
},
{
"activation": 13.964645385742188,
"uniprot_id": "Q1A244"
},
{
"activation": 1... | [
5207,
13482,
8819,
4627,
15458,
10956,
11224,
11353,
4818,
6971
] | 0.435727 |
4 | Summary: Intrinsically disordered, low‑complexity effector regions of metazoan transcriptional regulators and cofactors (activation/repression and cofactor‑binding segments), enriched in proline/serine/glutamine/glycine and PTM/SLiM hotspots, with little or no activation on folded DNA‑binding or SAM domains.
Activation... | Intrinsically disordered, low‑complexity effector regions of metazoan transcriptional regulators and cofactors (activation/repression and cofactor‑binding segments), enriched in proline/serine/glutamine/glycine and PTM/SLiM hotspots, with little or no activation on folded DNA‑binding or SAM domains. | Broad, contiguous regional activation across long N‑ or C‑terminal IDRs (often hundreds of residues; low pLDDT), with occasional peaks at short helices/coils and known linear motifs or PTM clusters; near‑zero activation over structured domains such as POU/homeobox, HMG box, SAM, and other folded cores. PolyQ tracts may... | Disorder | - POU-class homeobox transcription factors (e.g., BRN2/POU3F2): strong activation on the N‑terminal low‑complexity activation region; inactive on POU‑specific and homeobox DNA‑binding domains.
- TCF/LEF HMG box transcription factors (e.g., POP‑1): activation on the N‑terminal disordered regulatory region (includes phos... | 4,760,752 | 3.77716 | 16.171875 | [
{
"activation": 16.171875,
"uniref_id": "A0A9J7M6S5"
},
{
"activation": 16.0625,
"uniref_id": "A0A7K6F9T6"
},
{
"activation": 16.03125,
"uniref_id": "A0A7K8D2G4"
},
{
"activation": 16.015625,
"uniref_id": "A0A8C0VFA7"
},
{
"activation": 15.984375,
"uniref_id":... | [
{
"activation": 15.344257354736328,
"uniprot_id": "Q96NU1"
},
{
"activation": 15.296398162841797,
"uniprot_id": "Q1RNF8"
},
{
"activation": 14.820014953613281,
"uniprot_id": "P54259"
},
{
"activation": 14.220223426818848,
"uniprot_id": "Q8R089"
},
{
"activation": ... | [
1591,
6987,
6850,
7540,
10694,
3507,
6711,
11789,
10467,
5408
] | 0.347705 |
5 | Summary: Intrinsically disordered regulatory regions of transcription factors—especially fungal TFs—composed of low‑complexity Ser/Thr/Pro- and Gln/Asn‑rich segments that host short linear regulatory motifs (e.g., SP/TP phosphorylation clusters, YPX[LI] interaction/processing motifs, degron/processing boxes, occasional... | Intrinsically disordered regulatory regions of transcription factors—especially fungal TFs—composed of low‑complexity Ser/Thr/Pro- and Gln/Asn‑rich segments that host short linear regulatory motifs (e.g., SP/TP phosphorylation clusters, YPX[LI] interaction/processing motifs, degron/processing boxes, occasional Lys/Arg‑... | Broad regional activation over long, low‑pLDDT IDRs flanking or trailing DNA‑binding domains; minimal/absent signal on structured DBDs. Superimposed residue‑level peaks at protease boxes/cleavage sites and other SLiMs within the IDRs. Often spans N‑ or C‑terminal tails and large internal regulatory stretches. | Disorder | pH‑response TF PacC/RIM101: strong activation across the C‑terminal inhibitory propeptide, including the signaling protease box, cleavage site, and YPX[LI] motifs; no activation on the N‑terminal C2H2 zinc fingers. Myb‑like transcription factors: activation on the C‑terminal disordered regulatory tail outside the Myb r... | 6,227,829 | 3.508538 | 16.140625 | [
{
"activation": 16.140625,
"uniref_id": "A0A367K883"
},
{
"activation": 16.046875,
"uniref_id": "UPI0023007FF6"
},
{
"activation": 15.9921875,
"uniref_id": "A0A1C7NB31"
},
{
"activation": 15.953125,
"uniref_id": "I1BSX4"
},
{
"activation": 15.9296875,
"uniref_... | [
{
"activation": 14.969688415527344,
"uniprot_id": "P78871"
},
{
"activation": 14.574922561645508,
"uniprot_id": "P34233"
},
{
"activation": 13.977060317993164,
"uniprot_id": "Q753Y2"
},
{
"activation": 13.935336112976074,
"uniprot_id": "Q5A5Q6"
},
{
"activation": ... | [
1170,
2673,
5705,
2655,
13464,
6773,
14292,
8500,
8979,
5727
] | 0.6411 |
6 | Summary: Alpha-helical segments in transporter systems: predominantly hydrophobic transmembrane helices and adjacent loop–helix boundary motifs in multi-pass membrane proteins (EC 7 translocases; ABC and non-ABC) and 7TM receptors; also tolerates amphipathic helices within associated ABC ATPase (NBD) subunits
Activatio... | Alpha-helical segments in transporter systems: predominantly hydrophobic transmembrane helices and adjacent loop–helix boundary motifs in multi-pass membrane proteins (EC 7 translocases; ABC and non-ABC) and 7TM receptors; also tolerates amphipathic helices within associated ABC ATPase (NBD) subunits | Regional activation across many segments per protein (multiple TMs); strong signal on long hydrophobic alpha-helices; residue-level peaks at loop→TM transitions (N/C caps), at Trp/Tyr interface positions, and at Ser/Thr/Gly within hydrophobic runs; occasional activation on amphipathic helices in cytosolic ABC NBDs | Membrane-associated | - ABC heavy‑metal tolerance transporters (permease/TMD): activation on TM helices and at cytoplasmic loop→TM starts within the ABC transmembrane type‑1 domain
- Peptide antibiotic transporter SbmA: activation within a TM helix, at a periplasmic loop→TM transition, and on a periplasmic helical segment
- Bacteriocin/pept... | 11,381,374 | 2.905587 | 22.515625 | [
{
"activation": 22.515625,
"uniref_id": "UPI001BD4A070"
},
{
"activation": 22.375,
"uniref_id": "A0A6I3IVE1"
},
{
"activation": 22.375,
"uniref_id": "A0AB36JQY9"
},
{
"activation": 22.375,
"uniref_id": "UPI000A7DAC9B"
},
{
"activation": 22.328125,
"uniref_id":... | [
{
"activation": 21.230918884277344,
"uniprot_id": "O07549"
},
{
"activation": 21.20414161682129,
"uniprot_id": "P0AAG5"
},
{
"activation": 19.838315963745117,
"uniprot_id": "Q0P9C4"
},
{
"activation": 19.750431060791016,
"uniprot_id": "Q9X2W0"
},
{
"activation": 1... | [
3128,
6489,
8166,
5625,
3594,
1527,
11268,
9427,
7943,
5789
] | 0.300471 |
7 | Summary: Specific heptad-register signal in long alpha-helical coiled-coils: the feature marks one solvent-exposed position of the (abcdefg)n repeat (consistent with charged/polar e/g faces) across extended coiled-coil scaffolds common in eukaryotic cytoskeletal, centrosomal, nucleoporin, and endomembrane tether protei... | Specific heptad-register signal in long alpha-helical coiled-coils: the feature marks one solvent-exposed position of the (abcdefg)n repeat (consistent with charged/polar e/g faces) across extended coiled-coil scaffolds common in eukaryotic cytoskeletal, centrosomal, nucleoporin, and endomembrane tether proteins. | Residue-level sharp peaks recurring every 7 residues across long coiled-coil segments (often hundreds to >1000 aa); minimal in globular domains and unstructured regions unless they harbor latent coiled-coil propensity; activated residues are enriched for E/Q/K/R/S/T, consistent with a solvent-exposed coiled-coil face. | Structural motif | - Golgin-84 (Golgi tether): periodic peaks on the N-terminal cytosolic coiled-coil heptad register.
- Restin homolog (cytoskeletal coiled-coil): continuous 7-residue–spaced peaks across the long C-terminal coiled-coil.
- Cep290 (centrosomal/ciliary scaffold): heptad-pattern peaks throughout annotated coiled-coils and c... | 9,479,694 | 3.088413 | 20.390625 | [
{
"activation": 20.390625,
"uniref_id": "A0A850GJI8"
},
{
"activation": 20.359375,
"uniref_id": "A0A2S9XEN9"
},
{
"activation": 20.109375,
"uniref_id": "A0A956C7V5"
},
{
"activation": 19.9375,
"uniref_id": "UPI0035D4CAA0"
},
{
"activation": 19.890625,
"uniref_... | [
{
"activation": 16.945043563842773,
"uniprot_id": "Q9VJE5"
},
{
"activation": 16.81955909729004,
"uniprot_id": "Q1D823"
},
{
"activation": 16.67233657836914,
"uniprot_id": "I1RGD4"
},
{
"activation": 16.52066993713379,
"uniprot_id": "O94488"
},
{
"activation": 16.... | [
10979,
14900,
11423,
534,
4316,
1650,
9,
8058,
14528,
931
] | 0.920796 |
8 | Summary: Membrane-embedded transmembrane helical bundles of large multi-pass transporters/channels, with strongest preference for sterol/lipid-handling systems (sterol-sensing domain proteins and lipid exporters) and related multi-TM secretion/transport assemblies
Activation pattern: Domain-/regional-level activation c... | Membrane-embedded transmembrane helical bundles of large multi-pass transporters/channels, with strongest preference for sterol/lipid-handling systems (sterol-sensing domain proteins and lipid exporters) and related multi-TM secretion/transport assemblies | Domain-/regional-level activation concentrated in long, contiguous hydrophobic segments corresponding to transmembrane helices; broad coverage across entire TM bundles (e.g., the 5-helix sterol-sensing domain and additional TM clusters), with minimal signal in luminal/cytosolic loops; in large eukaryotic proteins, acti... | Membrane-associated | - Sterol-sensing domain proteins (Dispatched/Patched/NPC1/SCAP): activation spans the SSD 5-TM unit and adjacent TM clusters that form sterol/lipid conduits
- ABC lipid transporters (ABCA family): activation across both transmembrane domains (two ∼6-helix bundles), often strongest in the C-terminal TMD
- Mycobacterial ... | 4,040,523 | 3.941191 | 21.65625 | [
{
"activation": 21.65625,
"uniref_id": "A0A852VXV7"
},
{
"activation": 21.125,
"uniref_id": "A0A6V8CE66"
},
{
"activation": 20.984375,
"uniref_id": "A0A5N9HZR9"
},
{
"activation": 20.875,
"uniref_id": "A0A7M3ML86"
},
{
"activation": 20.71875,
"uniref_id": "A0A... | [
{
"activation": 19.143085479736328,
"uniprot_id": "P9WJU7"
},
{
"activation": 18.018360137939453,
"uniprot_id": "Q9VNJ5"
},
{
"activation": 18.008285522460938,
"uniprot_id": "P9WJU5"
},
{
"activation": 17.91112518310547,
"uniprot_id": "A0A0H3KP92"
},
{
"activation... | [
11118,
3223,
14639,
5875,
268,
6389,
12486,
15465,
5428,
13715
] | 0.176626 |
9 | Summary: Recognition of repetitive helical segments and hydrophobic/amphipathic motifs—most prominently the X/Y positions of collagen G–X–Y triple-helical repeats, but also contiguous hydrophobic alpha-helical transmembrane segments and proline/glycine-rich low-complexity stretches; predominant in secreted and membrane... | Recognition of repetitive helical segments and hydrophobic/amphipathic motifs—most prominently the X/Y positions of collagen G–X–Y triple-helical repeats, but also contiguous hydrophobic alpha-helical transmembrane segments and proline/glycine-rich low-complexity stretches; predominant in secreted and membrane proteins... | Regional activation over stretches: dense, domain-scale activation across collagenous triple-helix regions with zero or minimal signal on the invariant glycine (G) and peaks at X/Y (especially proline/hydrophobic residues); contiguous activation across single-pass or multi-pass transmembrane alpha-helices and amphipath... | Repeat | - Fibrillar collagens: strong activation along the triple-helical G–X–Y domain, peaking at X/Y positions (especially proline/hydroxyproline-like Y), with minimal signal on G.
- Light-harvesting complex alpha subunits (photosynthetic bacteria): activation along their single transmembrane alpha-helix.
- YhhN/TMEM86 lysop... | 50,660,038 | 1.412428 | 8.367188 | [
{
"activation": 8.3671875,
"uniref_id": "UPI002169DC94"
},
{
"activation": 8.359375,
"uniref_id": "A0A352SCY4"
},
{
"activation": 8.3515625,
"uniref_id": "U1S1F5"
},
{
"activation": 8.25,
"uniref_id": "A0A973J484"
},
{
"activation": 8.1328125,
"uniref_id": "A0... | [
{
"activation": 6.0440354347229,
"uniprot_id": "P86327"
},
{
"activation": 5.8930463790893555,
"uniprot_id": "Q28487"
},
{
"activation": 5.683255195617676,
"uniprot_id": "A0A182BSS4"
},
{
"activation": 5.659261703491211,
"uniprot_id": "P29164"
},
{
"activation": 5... | [
14900,
1650,
2304,
11423,
7,
8419,
4697,
6680,
4316,
10583
] | 0.371393 |
10 | Summary: Specific transmembrane alpha‑helical segments of multi‑pass solute transporters/efflux pumps, especially DMT/EamA (SLC35 and plastidic phosphate/nucleotide‑sugar transporters) and mitochondrial carriers (SLC25), characterized by hydrophobic helices enriched in aromatic anchors and occasional helix‑kink residue... | Specific transmembrane alpha‑helical segments of multi‑pass solute transporters/efflux pumps, especially DMT/EamA (SLC35 and plastidic phosphate/nucleotide‑sugar transporters) and mitochondrial carriers (SLC25), characterized by hydrophobic helices enriched in aromatic anchors and occasional helix‑kink residues (Gly/Pr... | Regional activation concentrated on one or a few individual transmembrane helices per protein, appearing as long contiguous blocks within those helices; residue‑level peaks frequently at aromatic residues (W/Y/F) and sometimes at kink/packing residues (G/P, occasionally H); minimal activation in soluble domains or targ... | Membrane-associated | - Plastidic Xylulose‑5‑phosphate/phosphate translocator (pPT; DMT/EamA repeat carrier): strong activation on specific EamA-domain TM helices, with negligible signal on the chloroplast transit peptide and most other helices.
- Bacterial guanidinium exporter (SMR family): primary activation on the C‑terminal part of TM2 ... | 8,163,288 | 3.237918 | 25.8125 | [
{
"activation": 25.8125,
"uniref_id": "J2RS97"
},
{
"activation": 25.796875,
"uniref_id": "UPI00298D3AB3"
},
{
"activation": 25.796875,
"uniref_id": "UPI00247630B0"
},
{
"activation": 25.765625,
"uniref_id": "A0A2T5AXM6"
},
{
"activation": 25.71875,
"uniref_id... | [
{
"activation": 24.473054885864258,
"uniprot_id": "D7A5Q8"
},
{
"activation": 24.180133819580078,
"uniprot_id": "B0B8F4"
},
{
"activation": 23.602218627929688,
"uniprot_id": "P0ADP5"
},
{
"activation": 23.49236488342285,
"uniprot_id": "P46136"
},
{
"activation": 2... | [
12225,
5037,
15380,
1370,
2596,
5846,
6816,
3258,
9479,
1930
] | 0.215546 |
11 | Summary: Charge-rich, low-complexity linker and terminal segments—typically acidic (Asp/Glu-enriched) but also tolerating basic/His runs—that are flexible/disordered or weakly helical and lie outside catalytic cores; these extensions are common in subunits of large macromolecular assemblies (ribosome, F1-ATP synthase, ... | Charge-rich, low-complexity linker and terminal segments—typically acidic (Asp/Glu-enriched) but also tolerating basic/His runs—that are flexible/disordered or weakly helical and lie outside catalytic cores; these extensions are common in subunits of large macromolecular assemblies (ribosome, F1-ATP synthase, chaperone... | Regional activation across long, contiguous stretches, with a strong preference for C-terminal tails and inter-domain linkers; often lower predicted structural confidence (IDR-like), sometimes forming short/loose helices; little to no activation in compact catalytic cores. | Disorder | • Holliday junction branch migration protein RuvA: activation on the low-confidence flexible linker between domains II and III and into the C-terminal region.
• Peptide deformylase: exclusive activation on the C-terminal tail/helix outside the catalytic/metal-binding core.
• Ribosomal proteins and rRNA biogenesis fac... | 9,059,907 | 3.133706 | 21.546875 | [
{
"activation": 21.546875,
"uniref_id": "A0A923W9B7"
},
{
"activation": 21.390625,
"uniref_id": "A0A975EJR3"
},
{
"activation": 21.296875,
"uniref_id": "UPI003626A34D"
},
{
"activation": 21.28125,
"uniref_id": "A0A4Q2YDQ8"
},
{
"activation": 21.265625,
"uniref... | [
{
"activation": 19.206684112548828,
"uniprot_id": "P0AB28"
},
{
"activation": 17.867006301879883,
"uniprot_id": "A0A1D6HQ92"
},
{
"activation": 17.418704986572266,
"uniprot_id": "Q9LT27"
},
{
"activation": 11.9529447555542,
"uniprot_id": "P56930"
},
{
"activation"... | [
1500,
7847,
146,
1447,
15166,
1855,
1801,
11458,
11355,
7931
] | 0.256214 |
12 | Summary: Long, low-complexity, intrinsically disordered terminal regions (often proline/glycine-rich) used as regulatory, adaptor-binding, and oligomerization segments that scaffold signaling and large macromolecular assemblies across taxa; these are strongly enriched in innate immune and microbial defense systems, but... | Long, low-complexity, intrinsically disordered terminal regions (often proline/glycine-rich) used as regulatory, adaptor-binding, and oligomerization segments that scaffold signaling and large macromolecular assemblies across taxa; these are strongly enriched in innate immune and microbial defense systems, but also occ... | Regional activation over tens to hundreds of residues, with a strong bias for N- or C-terminal stretches; frequently coincides with predicted disorder/low pLDDT and low-complexity composition; in some families, contiguous coverage includes the SEFIR/TIR module or the C-terminal catalytic lobe of nucleotidyl cyclases; t... | Disorder | - Interferon regulatory factors and other antiviral transcription factors: activation on disordered C-terminal transactivation/regulatory regions (e.g., IRF2/IRF3 C-termini), not the N-terminal DNA-binding domain.
- TIR/SEFIR family (IL-17 receptors, ACT1; plant/bacterial TIR/Thoeris): activation on the cytosolic SEFIR... | 7,143,808 | 3.371319 | 24.1875 | [
{
"activation": 24.1875,
"uniref_id": "UPI002AF6C780"
},
{
"activation": 24.109375,
"uniref_id": "UPI00360A451C"
},
{
"activation": 23.984375,
"uniref_id": "UPI00363A6DC8"
},
{
"activation": 23.9375,
"uniref_id": "A0A085ZFS2"
},
{
"activation": 23.890625,
"uni... | [
{
"activation": 20.718738555908203,
"uniprot_id": "A0A2T5Y4G4"
},
{
"activation": 20.31635093688965,
"uniprot_id": "A0A381HAP5"
},
{
"activation": 14.84177303314209,
"uniprot_id": "Q833J1"
},
{
"activation": 8.529245376586914,
"uniprot_id": "P0DXN8"
},
{
"activati... | [
3730,
14537,
5460,
12817,
2641,
3028,
1286,
14688,
1567,
7154
] | 0.164847 |
13 | Summary: Low-complexity, Ser/Thr/Pro-rich intrinsically disordered segments found in N-terminal tails, propeptides, targeting presequences (signal/transit peptides), and flexible linkers—serving as mucin-like O-glycosylated stalks in secreted/viral proteins, as phosphorylation/degron-rich regulatory regions in cytosoli... | Low-complexity, Ser/Thr/Pro-rich intrinsically disordered segments found in N-terminal tails, propeptides, targeting presequences (signal/transit peptides), and flexible linkers—serving as mucin-like O-glycosylated stalks in secreted/viral proteins, as phosphorylation/degron-rich regulatory regions in cytosolic protein... | Regional activation over contiguous clusters, typically starting near the N-terminus and spanning disordered tails/linkers/presequences; strong preference for S/T runs and SP/TP dipeptides; D/E and Gly frequently co-occur but are not required (transit peptides can be acidic-poor); low activation in well-folded cores bu... | Compositional bias | - Viral structural/attachment proteins: activation on Ser/Thr/Pro-rich mucin-like stalks and propeptides.
- Integrin alpha subunits: activation on N-terminal prodomain/extracellular low-complexity segments.
- Cystatins (stefins/type 2 cystatins): activation on flexible N-terminal segments and PEST-/PTS-like tracts.
- C... | 49,268,642 | 1.440277 | 14.296875 | [
{
"activation": 14.296875,
"uniref_id": "A0A7X1F9E5"
},
{
"activation": 14.2734375,
"uniref_id": "UPI0025EB4A01"
},
{
"activation": 14.2265625,
"uniref_id": "UPI0013EA073C"
},
{
"activation": 14.21875,
"uniref_id": "A0A0Q4KT97"
},
{
"activation": 14.2109375,
"... | [
{
"activation": 10.025796890258789,
"uniprot_id": "P03630"
},
{
"activation": 9.904528617858887,
"uniprot_id": "P14016"
},
{
"activation": 9.788032531738281,
"uniprot_id": "P44853"
},
{
"activation": 9.748165130615234,
"uniprot_id": "P03614"
},
{
"activation": 9.3... | [
14228,
5157,
5301,
7807,
6038,
12564,
12631,
6763,
7270,
777
] | 0.372983 |
14 | Summary: Terminus-proximal functional modules: the feature highlights a protein’s principal functional region—either the core of a folded domain or, in intrinsically disordered segments, embedded short linear interaction motifs—often at disorder-to-order boundaries and including catalytic/binding determinants (e.g., ac... | Terminus-proximal functional modules: the feature highlights a protein’s principal functional region—either the core of a folded domain or, in intrinsically disordered segments, embedded short linear interaction motifs—often at disorder-to-order boundaries and including catalytic/binding determinants (e.g., active-site... | Predominantly regional/domain-level activation in long contiguous blocks, frequently near a terminus; can split into several short segments within a domain; superimposed residue-level peaks at catalytic or binding residues; may extend into adjacent low-complexity coils when these harbor binding motifs | Domain | - Transcription-related regulator with large N-terminal IDR: activation confined to the structured C-terminal domain (ignores extensive disordered N-terminus)
- Proteasome subunit DSS1/SEM1 (Rpn15): activation across disordered N-terminal ubiquitin-binding SLiMs (UBS I/II) within low-pLDDT regions
- Photosystem II PsbP... | 7,715,843 | 3.29429 | 22.203125 | [
{
"activation": 22.203125,
"uniref_id": "A0A1C5HR93"
},
{
"activation": 22.1875,
"uniref_id": "A0A0D1YAD5"
},
{
"activation": 22.140625,
"uniref_id": "A0A5D0UYT9"
},
{
"activation": 22.125,
"uniref_id": "A0A917VBP5"
},
{
"activation": 22.046875,
"uniref_id": "... | [
{
"activation": 19.772228240966797,
"uniprot_id": "P30047"
},
{
"activation": 19.231605529785156,
"uniprot_id": "Q12467"
},
{
"activation": 18.898269653320312,
"uniprot_id": "Q14699"
},
{
"activation": 17.011259078979492,
"uniprot_id": "Q96CX2"
},
{
"activation": ... | [
8862,
7504,
1256,
2868,
6992,
15512,
15743,
15355,
7178,
9480
] | 0.143008 |
15 | Summary: Intrinsically disordered, low‑complexity repeat and linker tracts (rich in Ser/Thr/Pro/Gly/Asn) that act as flexible stalks/spacers in secreted and cell‑surface proteins—especially in Gram‑positive bacteria—connecting or flanking structured modules (e.g., catalytic domains, LysM, NEAT, Ig‑like, CBMs) rather th... | Intrinsically disordered, low‑complexity repeat and linker tracts (rich in Ser/Thr/Pro/Gly/Asn) that act as flexible stalks/spacers in secreted and cell‑surface proteins—especially in Gram‑positive bacteria—connecting or flanking structured modules (e.g., catalytic domains, LysM, NEAT, Ig‑like, CBMs) rather than the fo... | Extended regional activation across very long, repetitive low‑complexity segments; strongest just after the signal peptide and in inter‑domain linkers/stalks; repeated activation between tandem domains; little to no activation on well‑folded domains. | Disorder | - Staphylococcal autolysins with LysM repeats: activation on the long N‑terminal disordered region around the signal peptide cleavage and on short glycine/low‑complexity linkers between LysM repeats; not on the catalytic or LysM domains.
- MSCRAMM adhesins (e.g., clumping factors, Sdr family): activation on serine/aspa... | 8,203,838 | 3.232963 | 19.796875 | [
{
"activation": 19.796875,
"uniref_id": "UPI00290388ED"
},
{
"activation": 19.453125,
"uniref_id": "UPI0022B78D87"
},
{
"activation": 19.4375,
"uniref_id": "UPI0019057AE9"
},
{
"activation": 19.40625,
"uniref_id": "UPI0028FF730E"
},
{
"activation": 19.390625,
... | [
{
"activation": 18.24134063720703,
"uniprot_id": "P0DJM0"
},
{
"activation": 17.992229461669922,
"uniprot_id": "P16952"
},
{
"activation": 17.795677185058594,
"uniprot_id": "P17953"
},
{
"activation": 17.07499885559082,
"uniprot_id": "Q8Y3L4"
},
{
"activation": 16... | [
14681,
14413,
12785,
10780,
4884,
11481,
9087,
6795,
9268,
6205
] | 0.285472 |
16 | Summary: Detector for the mature cores of small, proteolytically processed and post‑translationally modified peptide effectors—especially RiPPs (lantibiotics, lasso peptides, thiopeptides, bacteriocins/pheromones)—and analogous cysteine‑rich toxin/effector segments. It responds to short Cys/Ser/Thr/Gly/Pro‑rich stretch... | Detector for the mature cores of small, proteolytically processed and post‑translationally modified peptide effectors—especially RiPPs (lantibiotics, lasso peptides, thiopeptides, bacteriocins/pheromones)—and analogous cysteine‑rich toxin/effector segments. It responds to short Cys/Ser/Thr/Gly/Pro‑rich stretches that h... | Strong, localized activation over short windows (tens of residues) that align with the mature peptide/core or a Cys‑centered modification motif; little to no activation on N‑terminal leaders/propeptides. Peaks typically start at or just after the cleavage site (often Gly/Gly or the first residue of the core) and center... | Post-translational modification | - Lantibiotic core peptides (lanthipeptides): activation spans the mature core containing dehydrated Ser/Thr and lanthionine/thioether crosslinks (e.g., flavovirin).
- Lasso peptides: activation on the core beginning at the conserved Gly and covering the isopeptide bond and aromatic lock residues (e.g., Astexin‑1).
- C... | 9,672,609 | 3.068267 | 14.070313 | [
{
"activation": 14.0703125,
"uniref_id": "A0AAE3R652"
},
{
"activation": 13.7734375,
"uniref_id": "A0A512AU80"
},
{
"activation": 13.421875,
"uniref_id": "A0A5M6CTI6"
},
{
"activation": 13.421875,
"uniref_id": "UPI002665E1EF"
},
{
"activation": 13.265625,
"uni... | [
{
"activation": 9.594944953918457,
"uniprot_id": "P29399"
},
{
"activation": 8.95433235168457,
"uniprot_id": "P0DQL4"
},
{
"activation": 8.867847442626953,
"uniprot_id": "P43683"
},
{
"activation": 8.629541397094727,
"uniprot_id": "P29827"
},
{
"activation": 8.437... | [
14993,
5563,
6258,
15924,
15052,
1472,
14495,
14008,
2765,
5171
] | 0.371451 |
17 | Summary: Long, charge-enriched amphipathic α‑helical scaffolds and associated charged IDRs in eukaryotic peripheral‑membrane and cytosolic adaptor/scaffold proteins that bind extended polyanionic surfaces (acidic membranes, F‑actin, nucleic acids) or short cargo/peptide motifs, often in a Ca2+-regulated manner (e.g., a... | Long, charge-enriched amphipathic α‑helical scaffolds and associated charged IDRs in eukaryotic peripheral‑membrane and cytosolic adaptor/scaffold proteins that bind extended polyanionic surfaces (acidic membranes, F‑actin, nucleic acids) or short cargo/peptide motifs, often in a Ca2+-regulated manner (e.g., annexin‑li... | Predominantly long, domain‑spanning activation over helix‑rich cores (sometimes multiple blocks in multi‑domain proteins); compositionally enriched for mixed acidic/basic and aliphatic residues consistent with amphipathic helices; additionally, strong activation on extended charged, low‑pLDDT disordered tails (e.g., C‑... | Domain | - Annexins/annexin‑like: activation across the α‑helical annexin core repeats that mediate Ca2+‑dependent binding to acidic phospholipid membranes.
- Actin cytoskeleton regulators (gelsolin/villin/flightless): activation over G‑domain helical bundles involved in Ca2+‑regulated actin and PIP2 interactions.
- ADF‑H famil... | 5,385,785 | 3.653802 | 21.15625 | [
{
"activation": 21.15625,
"uniref_id": "A0A7S2BLQ7"
},
{
"activation": 21.03125,
"uniref_id": "A0A914GTJ6"
},
{
"activation": 21.015625,
"uniref_id": "A0A6G0RV74"
},
{
"activation": 20.921875,
"uniref_id": "A0A5N4C1A1"
},
{
"activation": 20.84375,
"uniref_id":... | [
{
"activation": 18.699111938476562,
"uniprot_id": "P17063"
},
{
"activation": 18.567176818847656,
"uniprot_id": "Q9NFS4"
},
{
"activation": 18.163284301757812,
"uniprot_id": "C0HJG9"
},
{
"activation": 17.720613479614258,
"uniprot_id": "P08133"
},
{
"activation": ... | [
1216,
8405,
14937,
3499,
1334,
3236,
7147,
4917,
15961,
3725
] | 0.23308 |
18 | Summary: Feature for secretory/organellar precursors and membrane-associated catalytic proteins; strongest in intramembrane proteases and signal peptidases. Also detects lipid‑modifying enzymes via short Gly/Asp/His/Cys catalytic motifs; in soluble enzymes it fires primarily on conserved Gly/Asp-centered active-site lo... | Feature for secretory/organellar precursors and membrane-associated catalytic proteins; strongest in intramembrane proteases and signal peptidases. Also detects lipid‑modifying enzymes via short Gly/Asp/His/Cys catalytic motifs; in soluble enzymes it fires primarily on conserved Gly/Asp-centered active-site loops (e.g.... | - Enrichment in transmembrane helices, but broad, high-coverage activation is typical mainly for signal peptidases and intramembrane proteases; in other membrane proteins (channels/transporters, ZDHHC, single-pass anchors) activation is patchy/modest and dominated by local motifs.
- Superimposed residue-level peaks at ... | Catalytic function | - Intramembrane metalloproteases (Site-2): TM-bundle activation with a peak on the HEXXH motif.
- Rhomboid serine proteases: multiple TMs with a spike at the GxSxG catalytic motif.
- Signal/processing peptidases: stronger TM/signal-peptide activation with peaks at the Ser/Lys catalytic region.
- Sortase cysteine transp... | 6,829,442 | 3.416322 | 22.8125 | [
{
"activation": 22.8125,
"uniref_id": "A0A257NHU8"
},
{
"activation": 22.78125,
"uniref_id": "A0A7J5Z2Y1"
},
{
"activation": 22.5,
"uniref_id": "A0A5J9TZD1"
},
{
"activation": 22.484375,
"uniref_id": "A0A7J7DWT2"
},
{
"activation": 22.390625,
"uniref_id": "UPI... | [
{
"activation": 20.38900375366211,
"uniprot_id": "Q84VZ6"
},
{
"activation": 20.13740348815918,
"uniprot_id": "Q8H0W1"
},
{
"activation": 19.897932052612305,
"uniprot_id": "Q6AZD4"
},
{
"activation": 19.28263282775879,
"uniprot_id": "Q9M9Z2"
},
{
"activation": 18.... | [
1392,
14764,
8179,
15060,
10014,
5644,
2571,
2251,
3322,
13658
] | 0.158307 |
19 | Summary: Glycine- and acidic-residue–rich catalytic/RNA-contacting loop of pseudouridine synthases (TruB/Cbf5, TruA, Rlu/RsuA families)—centered on the universally conserved catalytic Asp and adjacent His/Pro-containing flexible segments that insert into tRNA/rRNA—with spillover to analogous gly-rich acidic loops in ot... | Glycine- and acidic-residue–rich catalytic/RNA-contacting loop of pseudouridine synthases (TruB/Cbf5, TruA, Rlu/RsuA families)—centered on the universally conserved catalytic Asp and adjacent His/Pro-containing flexible segments that insert into tRNA/rRNA—with spillover to analogous gly-rich acidic loops in other trans... | Primarily regional/domain-level activation across the mid-protein catalytic core (often ~100–300 aa), forming long contiguous stretches; superimposed residue-level peaks at short Gly clusters and acidic residues (Asp/Glu), frequently with nearby His and Pro | Catalytic function | - tRNA pseudouridine(55) synthases (TruB/Cbf5; including H/ACA RNP dyskerin and plant organellar forms): strong activation over the catalytic core; peaks on the gly/acidic loop bearing the invariant catalytic Asp
- tRNA pseudouridine synthases of the TruA family (Ψ at positions 38–40): activation on the central catalyt... | 7,901,980 | 3.270452 | 27.34375 | [
{
"activation": 27.34375,
"uniref_id": "A0A848TBN9"
},
{
"activation": 27.265625,
"uniref_id": "A0A6M1MK21"
},
{
"activation": 27.265625,
"uniref_id": "UPI0027AFE26A"
},
{
"activation": 27.25,
"uniref_id": "A0A433RZC6"
},
{
"activation": 27.234375,
"uniref_id"... | [
{
"activation": 25.621471405029297,
"uniprot_id": "Q3ECD0"
},
{
"activation": 25.19823455810547,
"uniprot_id": "Q9ZDR7"
},
{
"activation": 25.124401092529297,
"uniprot_id": "P0AA39"
},
{
"activation": 25.050342559814453,
"uniprot_id": "Q9UJJ7"
},
{
"activation": 2... | [
13816,
15277,
7873,
9001,
7075,
8918,
10277,
11317,
5990,
9799
] | 0.172951 |
20 | Summary: Anion-binding and metal-coordination hotspots: His/Cys- and basic-residue clusters (often with glycine-rich loops) that contact polyanionic ligands (DNA/RNA backbones, nucleotide/cofactor phosphates, PIP2, or carboxylates) and/or chelate divalent metals; also encompassing acidic Asp/Glu metal-ligating motifs (... | Anion-binding and metal-coordination hotspots: His/Cys- and basic-residue clusters (often with glycine-rich loops) that contact polyanionic ligands (DNA/RNA backbones, nucleotide/cofactor phosphates, PIP2, or carboxylates) and/or chelate divalent metals; also encompassing acidic Asp/Glu metal-ligating motifs (e.g., DDE... | Mixed motif- and region-level activation: sharp residue-level peaks on His, Cys, Lys/Arg, hallmark Asn, and acidic Asp/Glu in metal sites; short motif-centered hits (HNH/HUH, DDE/DEDD, DxD/HxH, Cys–His–Asp triads, glycine-rich phosphate-binding loops); and occasional multi–secondary-structure patches within allosteric/... | Ligand-binding site | - HMG-box transcription factors (SOX/TCF/LEF/SRY): single conserved Asn in the HMG box that makes minor-groove/phosphate contacts on DNA
- Viral DNA replication/packaging proteins and transposases (HUH and DDE/DEDD families, incl. PiggyBac-derived): catalytic metal-ligating motifs and adjacent basic DNA-binding patches... | 4,869,681 | 3.754537 | 19.78125 | [
{
"activation": 19.78125,
"uniref_id": "UPI0039C9E739"
},
{
"activation": 19.59375,
"uniref_id": "A0AAD7CN84"
},
{
"activation": 19.5,
"uniref_id": "A0A661QMK7"
},
{
"activation": 19.375,
"uniref_id": "A0A939AR37"
},
{
"activation": 19.359375,
"uniref_id": "A0... | [
{
"activation": 12.821662902832031,
"uniprot_id": "P09504"
},
{
"activation": 8.276521682739258,
"uniprot_id": "P0DJ29"
},
{
"activation": 7.536351203918457,
"uniprot_id": "Q8N328"
},
{
"activation": 7.478774547576904,
"uniprot_id": "Q46731"
},
{
"activation": 7.4... | [
15217,
15231,
9887,
9798,
2998,
11623,
7363,
1371,
7864,
15063
] | 0.248923 |
21 | Summary: Charged, low-complexity intrinsically disordered segments and flexible loops (often N- or C-terminal tails or inter-domain linkers) that frequently flank catalytic/nucleic-acid–binding modules and that sometimes contain short, family-defining motif signatures (e.g., VQ, CGPF, conserved Gly).
Activation pattern... | Charged, low-complexity intrinsically disordered segments and flexible loops (often N- or C-terminal tails or inter-domain linkers) that frequently flank catalytic/nucleic-acid–binding modules and that sometimes contain short, family-defining motif signatures (e.g., VQ, CGPF, conserved Gly). | Primarily regional activation on long contiguous, K/R/E/D/S/P-enriched low-complexity stretches (often low pLDDT) at termini or domain boundaries; supplemented by sharp residue-level peaks on short conserved motifs embedded in loops. Structured catalytic cores are generally weak, with occasional minor peaks near domain... | Disorder | - FAN1 nuclease: strong activation in a long C-terminal segment immediately before the VRR-NUC domain and in an N-terminal disordered region; minor peaks within UBZ4 zinc finger and near the VRR-NUC boundary.
- Lipoyl synthase (LipA/LIP1): activation confined to the disordered N-terminal tail; Radical SAM core not acti... | 6,763,834 | 3.425975 | 15.234375 | [
{
"activation": 15.234375,
"uniref_id": "UPI00386AB7CA"
},
{
"activation": 15.203125,
"uniref_id": "UPI002603F0C4"
},
{
"activation": 15.15625,
"uniref_id": "UPI00298E17AB"
},
{
"activation": 15.140625,
"uniref_id": "A0A7U0G8X4"
},
{
"activation": 15.1328125,
... | [
{
"activation": 4.793050765991211,
"uniprot_id": "D1KF50"
},
{
"activation": 4.717825412750244,
"uniprot_id": "Q06683"
},
{
"activation": 3.961649179458618,
"uniprot_id": "Q5FBC3"
},
{
"activation": 3.8135745525360107,
"uniprot_id": "Q9UIS9"
},
{
"activation": 3.6... | [
5582,
12416,
10990,
16230,
5892,
623,
6517,
14670,
10161,
10980
] | 0.638608 |
22 | Summary: Glycine-rich phosphate- or other oxyanion–binding loops that anchor nucleotides, phosphorylated ligands, or acidic/oxyanion moieties—covering Walker A/P‑loop (GxxxxGKT/S), Rossmann-like TGxGGxG, and analogous phosphate/pyrophosphate-binding motifs—across ATP/NAD/ADP‑ribose binders, kinases, and sugar‑phosphate... | Glycine-rich phosphate- or other oxyanion–binding loops that anchor nucleotides, phosphorylated ligands, or acidic/oxyanion moieties—covering Walker A/P‑loop (GxxxxGKT/S), Rossmann-like TGxGGxG, and analogous phosphate/pyrophosphate-binding motifs—across ATP/NAD/ADP‑ribose binders, kinases, and sugar‑phosphate enzymes,... | Predominantly regional/domain-level activation centered on glycine-rich oxyanion/phosphate-binding loops and their flanking β‑strand/α‑helix elements; residue-level peaks at conserved Lys/Thr/His that contact oxyanions (e.g., Walker A Lys). Often spans long continuous stretches that include substrate-binding residues. | Ligand-binding site | - Amino-acid kinases (glutamate 5-kinase/N-acetylglutamate kinase; AAK family): activation on the P-loop glycine-rich segment and adjacent helix, peaking at the Walker A Lys.
- Nucleoside monophosphate kinases (UMPK/UMP‑CMP kinase): activation on the canonical TGGxGGKT Walker A motif and neighboring secondary structure... | 7,302,172 | 3.349393 | 19.09375 | [
{
"activation": 19.09375,
"uniref_id": "A0A8S9H1Z6"
},
{
"activation": 18.796875,
"uniref_id": "A0A7X7DXJ2"
},
{
"activation": 18.359375,
"uniref_id": "UPI0031CF682C"
},
{
"activation": 18.3125,
"uniref_id": "UPI0035A69BAE"
},
{
"activation": 18.046875,
"unire... | [
{
"activation": 16.081117630004883,
"uniprot_id": "Q9T0I8"
},
{
"activation": 15.63368034362793,
"uniprot_id": "P27711"
},
{
"activation": 15.62169361114502,
"uniprot_id": "Q09117"
},
{
"activation": 14.905155181884766,
"uniprot_id": "Q5SKT7"
},
{
"activation": 14... | [
2261,
12454,
1740,
3955,
5324,
1523,
2245,
12742,
11371,
15027
] | 0.292435 |
23 | Summary: Solvent-exposed, non-transmembrane regions of membrane-associated and secreted proteins, with a bias toward ectodomains/periplasmic or lumenal surfaces and flexible linkers immediately outside single-pass anchors; additionally includes certain soluble enzyme cores (e.g., CHI-like). In transporters, activation ... | Solvent-exposed, non-transmembrane regions of membrane-associated and secreted proteins, with a bias toward ectodomains/periplasmic or lumenal surfaces and flexible linkers immediately outside single-pass anchors; additionally includes certain soluble enzyme cores (e.g., CHI-like). In transporters, activation targets e... | Regional activation across soluble ectodomains/catalytic cores of extracytosolic or lumenal proteins and selected soluble enzymes; focused patches on coil/turn-rich linkers proximal to transmembrane helices; residue-level peaks over His clusters in cytosolic or lumenal loops; little to no activation in transmembrane he... | Membrane-associated | - Gram-positive sortases: activation over the extracellular catalytic beta-barrel domain, not the membrane anchor or sorting signals
- Peptidoglycan/cell-envelope enzymes (e.g., transglycosylases, endopeptidases): broad activation across their secreted/periplasmic catalytic cores
- Flagellar basal body L-ring proteins ... | 5,077,939 | 3.71266 | 28.828125 | [
{
"activation": 28.828125,
"uniref_id": "A0A7H1MLE3"
},
{
"activation": 28.8125,
"uniref_id": "A0A0R2JJ55"
},
{
"activation": 28.8125,
"uniref_id": "D5T0N0"
},
{
"activation": 28.78125,
"uniref_id": "A0A1X0VFY1"
},
{
"activation": 28.75,
"uniref_id": "UPI00195... | [
{
"activation": 24.592893600463867,
"uniprot_id": "Q9XA15"
},
{
"activation": 24.52631950378418,
"uniprot_id": "Q2FV99"
},
{
"activation": 23.261489868164062,
"uniprot_id": "P94587"
},
{
"activation": 22.854164123535156,
"uniprot_id": "Q8Y8H5"
},
{
"activation": 2... | [
454,
6463,
6679,
8380,
6100,
14720,
11686,
8032,
15790,
5438
] | 0.145607 |
24 | Summary: Zinc-dependent deaminase catalytic domain (cytidine-deaminase–like fold) characterized by the HxE catalytic pair and a Cys–Cys zinc-binding motif, spanning nucleic acid and nucleotide deaminases (e.g., APOBEC/AID, ADAR/ADAT/TAD, dCMP/dCTP deaminases, RibD/DRAP) and also present in deaminase-like pseudoenzymes
... | Zinc-dependent deaminase catalytic domain (cytidine-deaminase–like fold) characterized by the HxE catalytic pair and a Cys–Cys zinc-binding motif, spanning nucleic acid and nucleotide deaminases (e.g., APOBEC/AID, ADAR/ADAT/TAD, dCMP/dCTP deaminases, RibD/DRAP) and also present in deaminase-like pseudoenzymes | Domain-level activation across the structured deaminase core, typically forming a long contiguous segment with residue-level peaks at the HxE catalytic pair and nearby cysteines of the Zn2+-binding motif; little to no activation in non-catalytic accessory domains (e.g., dsRBDs); position in sequence varies by family (N... | Domain | - ADAR RNA editases: strong activation over the C-terminal deaminase catalytic domain, not over N-terminal dsRNA-binding motifs (as in adr-1)
- tRNA adenosine deaminases (ADAT/TAD2/3): activation on the catalytic subunits’ deaminase fold that edits A34 in tRNA
- APOBEC/AID DNA cytidine deaminases and the bacterial toxi... | 5,494,234 | 3.633866 | 24.28125 | [
{
"activation": 24.28125,
"uniref_id": "UPI0039EC45A4"
},
{
"activation": 23.6875,
"uniref_id": "A0A139ASA6"
},
{
"activation": 23.34375,
"uniref_id": "A0AAD8DIP1"
},
{
"activation": 23.265625,
"uniref_id": "A0A9X0A215"
},
{
"activation": 23.265625,
"uniref_id... | [
{
"activation": 21.464881896972656,
"uniprot_id": "Q8BMD5"
},
{
"activation": 21.171945571899414,
"uniprot_id": "Q8TWU6"
},
{
"activation": 20.43832778930664,
"uniprot_id": "F4KH86"
},
{
"activation": 20.432058334350586,
"uniprot_id": "P16006"
},
{
"activation": 2... | [
9669,
596,
1859,
11055,
15435,
6226,
14042,
12351,
6365,
5503
] | 0.175682 |
25 | Summary: Soluble non-cytosolic domains of exported or imported proteins (periplasmic, extracellular, organellar lumen or intermembrane space), i.e., the mature regions adjacent to a targeting segment (signal peptide/lipobox, transit peptide, or signal anchor); enriched in cell-wall–acting hydrolases and Zn-dependent me... | Soluble non-cytosolic domains of exported or imported proteins (periplasmic, extracellular, organellar lumen or intermembrane space), i.e., the mature regions adjacent to a targeting segment (signal peptide/lipobox, transit peptide, or signal anchor); enriched in cell-wall–acting hydrolases and Zn-dependent metalloprot... | Regional activation across these non-cytosolic soluble domains with multiple residue-level peaks (often at D/E, H, and turn-forming G/P); little to no activation within targeting peptides, transmembrane helices, or cytosolic tails; occasional weak peaks at membrane boundaries; many discrete hotspots rather than a singl... | Membrane-associated | - Bacterial cell-wall remodeling enzymes (M23/M15 metallopeptidases, NlpC/P60 amidases, lysozymes; DD-peptidases/carboxypeptidases), including large periplasmic catalytic domains even when C-terminal to a single-pass signal anchor (class A/B PBPs)
- Phage/viral endolysins and tail-associated muralytic enzymes: activati... | 55,342,115 | 1.324031 | 12.5625 | [
{
"activation": 12.5625,
"uniref_id": "A0A966XK13"
},
{
"activation": 11.671875,
"uniref_id": "A0A418QGQ5"
},
{
"activation": 11.578125,
"uniref_id": "A0A519GW71"
},
{
"activation": 11.546875,
"uniref_id": "UPI0005B48A2B"
},
{
"activation": 11.4921875,
"uniref... | [
{
"activation": 9.488694190979004,
"uniprot_id": "Q8N119"
},
{
"activation": 9.383010864257812,
"uniprot_id": "P66948"
},
{
"activation": 9.344228744506836,
"uniprot_id": "P96499"
},
{
"activation": 9.297102928161621,
"uniprot_id": "Q9I2F1"
},
{
"activation": 9.16... | [
10227,
15327,
29,
11248,
3940,
8841,
10985,
1121,
9838,
7625
] | 0.410387 |
26 | Summary: Membrane-embedded α‑helical bundles that form conduits across membranes—predominantly cation channels and cation/proton or Na+/H+ exchangers—together with analogous multi‑pass helical modules of membrane enzymes (NOX/ferric reductases and processive polysaccharide synthases). The feature captures long hydropho... | Membrane-embedded α‑helical bundles that form conduits across membranes—predominantly cation channels and cation/proton or Na+/H+ exchangers—together with analogous multi‑pass helical modules of membrane enzymes (NOX/ferric reductases and processive polysaccharide synthases). The feature captures long hydrophobic trans... | Domain-level regional activation across extended clusters of transmembrane helices and short perimembrane loops (one long contiguous block). Peaks on hydrophobic TM residues with intermittent Gly/Pro helix breakers; often includes signal peptides and signal-anchor helices in secretory/membrane-targeted proteins. | Membrane-associated | - TRPM/TRP cation channels: activation over the S1–S6 pore-forming TM bundle including the pore helix/selectivity region.
- Cation/H+ and Na+/H+ antiporters (CPA/NHE families; NhaA/SOS1): activation across the 10–12 TM transport core with characteristic Gly/Pro-kinked helices.
- Potassium homeostasis systems (Trk/Ktr/K... | 4,829,212 | 3.762882 | 19.3125 | [
{
"activation": 19.3125,
"uniref_id": "A0A8X7N108"
},
{
"activation": 19.25,
"uniref_id": "V8P804"
},
{
"activation": 19.234375,
"uniref_id": "M7BGZ4"
},
{
"activation": 19.234375,
"uniref_id": "A0A7S3Z0I3"
},
{
"activation": 19.203125,
"uniref_id": "A0AAN9YU8... | [
{
"activation": 18.40638542175293,
"uniprot_id": "Q9BXP2"
},
{
"activation": 18.1925048828125,
"uniprot_id": "P55011"
},
{
"activation": 17.78297996520996,
"uniprot_id": "O60146"
},
{
"activation": 17.55760955810547,
"uniprot_id": "P34261"
},
{
"activation": 17.49... | [
15758,
11654,
7422,
7972,
2570,
10638,
14634,
5473,
15669,
7248
] | 0.208779 |
27 | Summary: Residue-level signature of catalytic and energy-coupling elements of molecular machines, predominantly ATP/GTP-dependent, with strongest expression in P-type ATPases; it highlights small/hydrophobic and acidic/polar residues that form nucleotide/Mg2+-binding and catalytic loops (e.g., Gly/Thr/Asp in Walker/swi... | Residue-level signature of catalytic and energy-coupling elements of molecular machines, predominantly ATP/GTP-dependent, with strongest expression in P-type ATPases; it highlights small/hydrophobic and acidic/polar residues that form nucleotide/Mg2+-binding and catalytic loops (e.g., Gly/Thr/Asp in Walker/switch or P-... | Residue-level peaks dispersed across structured catalytic cores and selected transmembrane helices rather than a single contiguous motif; enriched at catalytic Asp residues (e.g., P-type DKTGT/TGES region), Gly/Thr near nucleotide-binding/switch loops, and Mg2+-liganding Asp/Glu; biased toward A/V/I/L/G plus D/T/S; spa... | Catalytic function | - P-type heavy-metal ATPases (e.g., Cu-exporting P1B pumps): peaks on the P-domain catalytic Asp and adjacent Gly/Thr, Mg2+-binding acidic residues in the cytosolic catalytic core, and selected transmembrane residues (including metal-binding Cys) along the ion pathway.
- P4-ATPase phospholipid flippases: strong activat... | 53,586,781 | 1.356263 | 9.609375 | [
{
"activation": 9.609375,
"uniref_id": "A0A976HGE6"
},
{
"activation": 9.546875,
"uniref_id": "A0A2E8XGS5"
},
{
"activation": 9.546875,
"uniref_id": "A0A966ENK1"
},
{
"activation": 9.53125,
"uniref_id": "A0A963TQ78"
},
{
"activation": 9.5078125,
"uniref_id": "... | [
{
"activation": 8.264577865600586,
"uniprot_id": "Q9HV55"
},
{
"activation": 8.139707565307617,
"uniprot_id": "P37107"
},
{
"activation": 8.137439727783203,
"uniprot_id": "P10121"
},
{
"activation": 8.13724136352539,
"uniprot_id": "Q726P7"
},
{
"activation": 8.128... | [
15762,
15055,
10594,
15880,
14586,
10752,
13563,
719,
12341,
1326
] | 0.397506 |
28 | Summary: Acidic/histidine‑enriched, beta‑strand–rich ligand/metal‑binding domains—most prominently jelly‑roll/β‑roll/DSBH cores (cupin and 2‑oxoglutarate oxygenases) and CNBD/CNBHD modules—plus extended zinc‑finger repeat regions; the feature tracks the domain‑level context around binding pockets that use His/Asp/Glu (... | Acidic/histidine‑enriched, beta‑strand–rich ligand/metal‑binding domains—most prominently jelly‑roll/β‑roll/DSBH cores (cupin and 2‑oxoglutarate oxygenases) and CNBD/CNBHD modules—plus extended zinc‑finger repeat regions; the feature tracks the domain‑level context around binding pockets that use His/Asp/Glu (and nearb... | Domain‑level activation across long contiguous stretches covering β‑core sheets and adjacent loops; strongest where His/Asp/Glu and aromatic (W/Y) residues cluster around metal/anionic‑ligand sites. In zinc‑finger arrays, activation spans the repeat region but shows consistent dips at Zn‑coordinating cysteines. | Domain | - JmjC 2‑oxoglutarate/Fe(II) oxygenases: activation over the DSBH catalytic core surrounding the Hx(D/E)…H metal‑binding motif and 2‑OG pocket.
- Cupin‑family dioxygenases (acireductone dioxygenase, 3‑hydroxyanthranilate dioxygenase): activation across the cupin β‑barrel with His/Glu metal‑ligating triads and aromatic‑... | 19,882,249 | 2.347738 | 12.109375 | [
{
"activation": 12.109375,
"uniref_id": "UPI00374E65C2"
},
{
"activation": 12.1015625,
"uniref_id": "UPI000DF8098E"
},
{
"activation": 12.0859375,
"uniref_id": "A0A5C7MAU4"
},
{
"activation": 12.046875,
"uniref_id": "A0A9P3VL88"
},
{
"activation": 12.015625,
"... | [
{
"activation": 9.615205764770508,
"uniprot_id": "D4GU69"
},
{
"activation": 8.902103424072266,
"uniprot_id": "Q9XC60"
},
{
"activation": 8.845661163330078,
"uniprot_id": "Q2U6D4"
},
{
"activation": 8.743637084960938,
"uniprot_id": "Q9NWT6"
},
{
"activation": 8.50... | [
3616,
8041,
6937,
6292,
10526,
11592,
11495,
4525,
10035,
2200
] | 0.443568 |
29 | Summary: Residues within well‑ordered beta‑strands of beta‑sheet–rich folds—especially beta‑sandwich/jellyroll and beta‑propeller domains—used broadly in extracellular lectin‑like modules and intracellular scaffolds; in secreted beta‑sandwiches (e.g., pentraxins), the peaks often include disulfide‑bearing cysteines and... | Residues within well‑ordered beta‑strands of beta‑sheet–rich folds—especially beta‑sandwich/jellyroll and beta‑propeller domains—used broadly in extracellular lectin‑like modules and intracellular scaffolds; in secreted beta‑sandwiches (e.g., pentraxins), the peaks often include disulfide‑bearing cysteines and adjacent... | Domain‑focused, residue‑level peaks clustered along multiple beta‑strands (DSSP E) inside a single domain (PTX, LNS, SPRY, catalytic beta‑propeller), with high structural confidence and little activation in loops/helices; typically not centered on catalytic residues but on structural strands, sometimes highlighting dis... | Structural motif | Sialidases/neuraminidases: multiple beta‑strands in the catalytic beta‑propeller, excluding the active site and BNR repeats; Exolysin/toxin catalytic domains: contiguous beta‑strands within the C‑terminal catalytic region; Pentraxins (C‑reactive protein; adhesion GPCR N‑terminal PTX domain): disulfide‑forming cysteines... | 39,567,746 | 1.659551 | 18.234375 | [
{
"activation": 18.234375,
"uniref_id": "A0A9D6TGD8"
},
{
"activation": 18.21875,
"uniref_id": "G8PDL0"
},
{
"activation": 18.109375,
"uniref_id": "A0A968NPK9"
},
{
"activation": 18.03125,
"uniref_id": "A0A9D6FRP1"
},
{
"activation": 17.9375,
"uniref_id": "X1D... | [
{
"activation": 15.67428970336914,
"uniprot_id": "F4JD14"
},
{
"activation": 15.566536903381348,
"uniprot_id": "P85088"
},
{
"activation": 15.449434280395508,
"uniprot_id": "Q45914"
},
{
"activation": 15.234633445739746,
"uniprot_id": "Q9LCC0"
},
{
"activation": 1... | [
1529,
5391,
10227,
3940,
25,
11248,
5011,
8841,
13255,
7625
] | 0.386591 |
30 | Summary: N-terminal ligand-binding lobe of clamshell/alpha–beta domains, most strongly the periplasmic substrate-binding protein (SBP) fold used by bacterial ABC importers—especially Zn2+-chelate ZnuA/AdcA—recognizing the post–signal peptide binding domain and its histidine/tyrosine-lined metal-coordination environment... | N-terminal ligand-binding lobe of clamshell/alpha–beta domains, most strongly the periplasmic substrate-binding protein (SBP) fold used by bacterial ABC importers—especially Zn2+-chelate ZnuA/AdcA—recognizing the post–signal peptide binding domain and its histidine/tyrosine-lined metal-coordination environment; weaker ... | Predominantly domain-level, long contiguous activation starting immediately after a signal peptide in exported/periplasmic proteins and spanning much of the mature binding domain; residue-level peaks at metal-coordinating His/Tyr loops in zinc SBPs. In non-SBP families, bias toward N-terminal binding/catalytic subdomai... | Domain | - Periplasmic metal-chelate SBPs (ZnuA/AdcA/TroA-like): strong activation across both lobes of the Venus-flytrap binding domain right after the signal peptide; peaks on His/Tyr metal-binding motifs.
- Other periplasmic SBPs (sugar/amino acid/ion-binding): activation across the SBP clamshell domain following the signal ... | 4,632,615 | 3.804444 | 25.171875 | [
{
"activation": 25.171875,
"uniref_id": "A0A2Z6MAC1"
},
{
"activation": 25.109375,
"uniref_id": "A0A438INM6"
},
{
"activation": 24.96875,
"uniref_id": "A0A453J6L8"
},
{
"activation": 24.921875,
"uniref_id": "Q10SK0"
},
{
"activation": 24.859375,
"uniref_id": "... | [
{
"activation": 19.738725662231445,
"uniprot_id": "P37028"
},
{
"activation": 19.676124572753906,
"uniprot_id": "P44206"
},
{
"activation": 18.321266174316406,
"uniprot_id": "Q7A652"
},
{
"activation": 18.312828063964844,
"uniprot_id": "P0AEL6"
},
{
"activation": ... | [
10293,
981,
10345,
1320,
898,
6528,
15648,
12045,
12156,
923
] | 0.171932 |
31 | Summary: Conserved Ig-like domain signature around the intradomain disulfide: the Y–x–C (often Y–T–C) motif and adjacent loop/β-strand region (F-strand/FG loop in Ig numbering) in extracellular V-set and C2-set immunoglobulin folds across IgSF receptors, ligands, and viral Ig mimics
Activation pattern: Repeated, short ... | Conserved Ig-like domain signature around the intradomain disulfide: the Y–x–C (often Y–T–C) motif and adjacent loop/β-strand region (F-strand/FG loop in Ig numbering) in extracellular V-set and C2-set immunoglobulin folds across IgSF receptors, ligands, and viral Ig mimics | Repeated, short residue-level peaks in each Ig-like domain of multi-Ig proteins; maxima sit on or just N-terminal to the conserved cysteine that forms the intradomain disulfide, commonly at a Tyr–x–Cys triad and neighboring residues. Peaks fall in the loop-to-β transition or the β-strand itself, often preceded by an ac... | Sequence motif | - Siglecs (e.g., sialic acid-binding Ig-like lectins): sharp peaks in the N-terminal V-set ligand-binding domain and analogous positions in downstream C2 domains near the disulfide-proximal YxC motif.
- Fc receptor-like proteins: peaks on the C2-set β-strand immediately N-terminal to the disulfide cysteine within each ... | 23,895,508 | 2.163875 | 25.890625 | [
{
"activation": 25.890625,
"uniref_id": "A0A815XUP8"
},
{
"activation": 25.78125,
"uniref_id": "A0A8S2ELV5"
},
{
"activation": 25.671875,
"uniref_id": "A0AAW0N773"
},
{
"activation": 25.65625,
"uniref_id": "A0A8C4W6J6"
},
{
"activation": 25.65625,
"uniref_id":... | [
{
"activation": 24.995285034179688,
"uniprot_id": "Q86VR7"
},
{
"activation": 24.963459014892578,
"uniprot_id": "Q61790"
},
{
"activation": 24.92469596862793,
"uniprot_id": "A6QQC6"
},
{
"activation": 24.890798568725586,
"uniprot_id": "P97546"
},
{
"activation": 2... | [
169,
586,
2016,
4385,
284,
5689,
4956,
1663,
4357,
14936
] | 0.659352 |
32 | Summary: Extracytoplasmic (periplasmic/lumen-facing) domains of exported and lipoprotein factors that assemble, remodel, or scaffold the bacterial cell envelope and analogous organellar membranes (chloroplast thylakoid lumen, mitochondrial inner-membrane/IMS), spanning peptidoglycan enzymes, outer-/inner-membrane and f... | Extracytoplasmic (periplasmic/lumen-facing) domains of exported and lipoprotein factors that assemble, remodel, or scaffold the bacterial cell envelope and analogous organellar membranes (chloroplast thylakoid lumen, mitochondrial inner-membrane/IMS), spanning peptidoglycan enzymes, outer-/inner-membrane and flagellar ... | Regional activation across multiple noncontiguous segments of the extracytoplasmic domain, concentrated in acidic, turn-prone loop regions that contact partners/substrates; weak on transmembrane helices or cytosolic tails; sometimes includes residues near signal peptide/lipobox or periplasm-exposed ring/PG‑binding surf... | Membrane-associated | - SPFH-domain scaffolds (prohibitin/stomatin/HflK/C): activation on large periplasmic/lumenal scaffold domains between the single-pass TM anchor and coiled-coil regions.
- Gram-positive sortases (A/C): activation on extracellular catalytic-domain loops and the substrate (LPXTG) binding groove.
- Flagellar basal-body co... | 42,458,206 | 1.589045 | 11.742188 | [
{
"activation": 11.7421875,
"uniref_id": "A0A3M5FN00"
},
{
"activation": 11.7109375,
"uniref_id": "UPI002E27F85A"
},
{
"activation": 11.578125,
"uniref_id": "A0A3C1HEW5"
},
{
"activation": 11.5703125,
"uniref_id": "A0A6P2ALU8"
},
{
"activation": 11.515625,
"un... | [
{
"activation": 10.091876029968262,
"uniprot_id": "P86220"
},
{
"activation": 9.846056938171387,
"uniprot_id": "Q7BHL7"
},
{
"activation": 9.82711124420166,
"uniprot_id": "P0ABC7"
},
{
"activation": 9.683174133300781,
"uniprot_id": "P96499"
},
{
"activation": 9.55... | [
1569,
3936,
9836,
6692,
5942,
11548,
8531,
6136,
13000,
16160
] | 0.446869 |
33 | Summary: N-terminal alpha-helical segment—most often a signal peptide/signal-anchor H-region, but also the first amphipathic/packing helix of soluble proteins; the helix can be hydrophobic or amphipathic.
Activation pattern: Regional activation concentrated near the N-terminus (typically within residues 1–40), forming ... | N-terminal alpha-helical segment—most often a signal peptide/signal-anchor H-region, but also the first amphipathic/packing helix of soluble proteins; the helix can be hydrophobic or amphipathic. | Regional activation concentrated near the N-terminus (typically within residues 1–40), forming contiguous runs over hydrophobic residues (Leu, Val, Ile, Phe, Ala) with occasional Gly; window length varies (short helices to ~20–25 aa H-regions). | Post-translational modification | - Plant non-specific lipid transfer proteins/allergens: activation on the N-terminal signal peptide H-region (hydrophobic core and small residues near the cleavage site)
- Bacterial anti-sigma factor regulators: activation on their single-pass transmembrane signal-anchor helix (position varies, often C-terminal) or on ... | 105,565,458 | 0.678234 | 10.476563 | [
{
"activation": 10.4765625,
"uniref_id": "A0A517QUZ9"
},
{
"activation": 10.40625,
"uniref_id": "A0A517SN96"
},
{
"activation": 10.390625,
"uniref_id": "UPI00135836BE"
},
{
"activation": 10.375,
"uniref_id": "A0A9X3MM99"
},
{
"activation": 10.3671875,
"uniref_... | [
{
"activation": 8.88333511352539,
"uniprot_id": "E6Z0R4"
},
{
"activation": 8.373893737792969,
"uniprot_id": "P32727"
},
{
"activation": 8.139012336730957,
"uniprot_id": "P62279"
},
{
"activation": 8.064634323120117,
"uniprot_id": "G1SP51"
},
{
"activation": 7.996... | [
11972,
12000,
9464,
14028,
16158,
934,
12877,
14046,
2568,
2344
] | 0.189999 |
34 | Summary: A structural-propensity feature for long, helix-poor, extended segments—preferentially β‑strand/turn–rich blocks enriched in small hydrophobic residues and glycine (often with Gly repeats), and in some cases extended low‑complexity repeat tracts (e.g., P/S/Y‑rich CTDs)—that occur as contiguous stretches within... | A structural-propensity feature for long, helix-poor, extended segments—preferentially β‑strand/turn–rich blocks enriched in small hydrophobic residues and glycine (often with Gly repeats), and in some cases extended low‑complexity repeat tracts (e.g., P/S/Y‑rich CTDs)—that occur as contiguous stretches within large en... | Regional activation over long contiguous blocks (often multiple per protein), with residue‑level peaks within β‑strands and adjacent turns; also activates on extended low‑complexity repeat regions (e.g., polymerase CTDs). | Structural motif | - Aconitate hydratase/aconitase: extended β‑sheet core segments.
- 2‑methyl‑aconitate isomerase (branched‑chain/leucine pathway): β‑strand blocks in the structured core (e.g., 136–179 and 349–377; peak near V152).
- Isopropylmalate isomerase/homoaconitase: β‑strand–rich regions within the catalytic core.
- NAD(P)HX deh... | 23,212,093 | 2.192892 | 10.96875 | [
{
"activation": 10.96875,
"uniref_id": "UPI00345A80B8"
},
{
"activation": 10.8671875,
"uniref_id": "A0A2M7T9C7"
},
{
"activation": 10.859375,
"uniref_id": "UPI0019448001"
},
{
"activation": 10.796875,
"uniref_id": "A0A523RMF1"
},
{
"activation": 10.7734375,
"u... | [
{
"activation": 9.491840362548828,
"uniprot_id": "P36683"
},
{
"activation": 9.358195304870605,
"uniprot_id": "P20004"
},
{
"activation": 9.18800163269043,
"uniprot_id": "O04916"
},
{
"activation": 9.127878189086914,
"uniprot_id": "P21399"
},
{
"activation": 8.982... | [
537,
7145,
3480,
9628,
6650,
394,
12267,
2130,
7618,
1433
] | 0.493338 |
35 | Summary: Mature extracellular/secreted ectodomains of secretory‑pathway proteins (soluble secreted factors and the lumenal portions of surface‑anchored proteins), activating immediately downstream of the signal peptide and covering the long folded/exported region while avoiding the signal peptide, transmembrane segment... | Mature extracellular/secreted ectodomains of secretory‑pathway proteins (soluble secreted factors and the lumenal portions of surface‑anchored proteins), activating immediately downstream of the signal peptide and covering the long folded/exported region while avoiding the signal peptide, transmembrane segments, cytoso... | Domain‑level activation across long, contiguous segments starting just after the signal peptide; strong over the main extracellular/ecto domain (including glycosylation‑site–bearing loops and repeat‑rich regions), with little to no activation on the N‑terminal signal peptide, transmembrane helices, propeptide/anchor pe... | Domain | - Trypanosome variant surface glycoproteins (VSGs): high activation across the mature folded ectodomain; none on the N‑terminal signal peptide or the C‑terminal GPI‑attachment/propeptide.
- Oomycete RxLR effectors: activation over the secreted effector domain following the signal peptide and host‑targeting motif.
- Yea... | 7,364,068 | 3.340953 | 15.6875 | [
{
"activation": 15.6875,
"uniref_id": "UPI0025E72676"
},
{
"activation": 15.6640625,
"uniref_id": "UPI0025DAEAAD"
},
{
"activation": 15.5703125,
"uniref_id": "UPI0020CF409D"
},
{
"activation": 15.484375,
"uniref_id": "A0AAE7M7F2"
},
{
"activation": 15.4296875,
... | [
{
"activation": 10.62155532836914,
"uniprot_id": "O34344"
},
{
"activation": 10.285271644592285,
"uniprot_id": "Q07337"
},
{
"activation": 6.760920524597168,
"uniprot_id": "P0DXF3"
},
{
"activation": 6.385785102844238,
"uniprot_id": "P19837"
},
{
"activation": 5.9... | [
7747,
16264,
12604,
8222,
6282,
70,
12890,
4854,
2693,
14390
] | 0.431377 |
36 | Summary: RNA pseudouridine synthases (EC 5.4.99.-) across TruA/TruB/RsuA/RluA/TruD/Pus10/RPUSD families, with strong representation of organelle-targeted (mitochondrial/chloroplast) enzymes; the feature recognizes the conserved pseudouridine synthase catalytic core and associated RNA‑binding surfaces, with secondary, w... | RNA pseudouridine synthases (EC 5.4.99.-) across TruA/TruB/RsuA/RluA/TruD/Pus10/RPUSD families, with strong representation of organelle-targeted (mitochondrial/chloroplast) enzymes; the feature recognizes the conserved pseudouridine synthase catalytic core and associated RNA‑binding surfaces, with secondary, weaker act... | Predominantly domain-level activation spanning the pseudouridine synthase catalytic fold (including the catalytic Asp-containing loop and adjacent RNA-binding elements); in eukaryotic organelle precursors, long N‑terminal stretches (transit peptides) are additionally activated; weaker, sparse activation occurs in some ... | Domain | - TruB-family tRNA Ψ55 synthases — strong activation across catalytic core and RNA-binding subdomains
- TruA-family tRNA Ψ38–40 synthases — strong domain-level activation of the PUS core
- RsuA-family 16S rRNA Ψ516 synthases — activation over the conserved catalytic domain
- RluA/RluB/RluC rRNA/tRNA pseudouridine synth... | 5,393,523 | 3.652366 | 25.640625 | [
{
"activation": 25.640625,
"uniref_id": "UPI00203E9948"
},
{
"activation": 25.28125,
"uniref_id": "I0YKT8"
},
{
"activation": 25.234375,
"uniref_id": "Q00TL6"
},
{
"activation": 25.234375,
"uniref_id": "A0A9D1Q228"
},
{
"activation": 25.234375,
"uniref_id": "A... | [
{
"activation": 23.756132125854492,
"uniprot_id": "Q9LT72"
},
{
"activation": 23.640893936157227,
"uniprot_id": "Q9SVS0"
},
{
"activation": 23.410980224609375,
"uniprot_id": "Q06244"
},
{
"activation": 23.372941970825195,
"uniprot_id": "Q9ZDR7"
},
{
"activation": ... | [
9212,
14151,
11755,
12214,
9209,
3102,
3235,
5463,
13071,
12544
] | 0.152207 |
37 | Summary: Short, basic/aromatic recognition patches (often a short α-helix plus adjacent loop) that form ligand-binding interface “hot spots,” typically engaging phosphate-rich nucleotides/nucleic acids or ring-rich peptides via electrostatic and stacking contacts; frequently include histidines and acidic residues near ... | Short, basic/aromatic recognition patches (often a short α-helix plus adjacent loop) that form ligand-binding interface “hot spots,” typically engaging phosphate-rich nucleotides/nucleic acids or ring-rich peptides via electrostatic and stacking contacts; frequently include histidines and acidic residues near R/K and Y... | Regional activation on short (≈8–20 aa) contiguous segments, with residue-level peaks at R/K/H and Y/W (often alongside E/D). Multiple discrete patches can occur within one protein and can appear at N-termini, internal segments, or C-termini, commonly mapping to short helices or helix-capping loops at binding interface... | Ligand-binding site | - Cyclic di-GMP enzymes (GGDEF diguanylate cyclases and EAL/HD-GYP phosphodiesterases): activation on short interface segments such as the GGDEF I-site (RxxD/adjacent helix) and EAL/HD-GYP catalytic/recognition loops that contact c-di-GMP via basic and aromatic residues.
- HTH-type transcriptional regulators: recogniti... | 18,634,876 | 2.41253 | 16.640625 | [
{
"activation": 16.640625,
"uniref_id": "A0A3A6NAY8"
},
{
"activation": 16.59375,
"uniref_id": "A0A432S2R5"
},
{
"activation": 16.578125,
"uniref_id": "A0A1P8US95"
},
{
"activation": 16.515625,
"uniref_id": "UPI0033418C64"
},
{
"activation": 16.515625,
"uniref... | [
{
"activation": 13.19797420501709,
"uniprot_id": "P37671"
},
{
"activation": 13.107840538024902,
"uniprot_id": "Q6XL52"
},
{
"activation": 12.712759971618652,
"uniprot_id": "P42968"
},
{
"activation": 12.100174903869629,
"uniprot_id": "P75990"
},
{
"activation": 1... | [
12698,
2943,
11202,
5865,
1386,
6163,
3344,
868,
11123,
14878
] | 0.50222 |
38 | Summary: Intrinsic, short, N‑terminal disordered tails of RNA polymerase sigma-factor systems—i.e., the unstructured pro/targeting segments immediately preceding the conserved sigma domains—most strongly in bacterial sigma factors (especially ECF, but also primary, sporulation, and flagellar) and also in related anti‑s... | Intrinsic, short, N‑terminal disordered tails of RNA polymerase sigma-factor systems—i.e., the unstructured pro/targeting segments immediately preceding the conserved sigma domains—most strongly in bacterial sigma factors (especially ECF, but also primary, sporulation, and flagellar) and also in related anti‑sigma regu... | Residue-level peaks at the extreme N terminus (often at M1 and the next ~5–15 residues), with occasional additional hits on short internal low‑pLDDT coil segments; little to no activation over folded sigma domains (RNAP‑binding region 2, HTH region 4). Enrichment in Actinobacteria and Firmicutes; weaker, sporadic activ... | Disorder | - ECF sigma factors: activation on the short unstructured N‑terminal tail preceding region 2; conserved core (σ2/σ4) not activated.
- Sporulation sigma factors (e.g., SigE/SigK/SigH): activation on N‑terminal pro‑peptides or disordered N‑terminal extensions; no signal on core DNA/RNAP‑binding motifs.
- Flagellar sigma ... | 4,247,500 | 3.891235 | 17.328125 | [
{
"activation": 17.328125,
"uniref_id": "A0A953XDQ1"
},
{
"activation": 16.59375,
"uniref_id": "UPI0033390725"
},
{
"activation": 16.59375,
"uniref_id": "UPI003336E983"
},
{
"activation": 16.09375,
"uniref_id": "UPI00333A34D8"
},
{
"activation": 16.078125,
"un... | [
{
"activation": 14.225525856018066,
"uniprot_id": "Q9WX11"
},
{
"activation": 12.36990737915039,
"uniprot_id": "O53590"
},
{
"activation": 12.1463623046875,
"uniprot_id": "P9WGG7"
},
{
"activation": 11.01025390625,
"uniprot_id": "P9WGG9"
},
{
"activation": 10.3246... | [
442,
10621,
9516,
6420,
4154,
16048,
7394,
12558,
4142,
3427
] | 0.228913 |
39 | Summary: Eukaryotic low-complexity regulatory/interaction regions—intrinsically disordered or semi-structured activation/repression domains and auxiliary co-regulatory modules (typically acidic and/or S/T/Q/P/Gly-rich) that lie outside compact DNA-binding or catalytic cores; includes defined modules such as the C-termi... | Eukaryotic low-complexity regulatory/interaction regions—intrinsically disordered or semi-structured activation/repression domains and auxiliary co-regulatory modules (typically acidic and/or S/T/Q/P/Gly-rich) that lie outside compact DNA-binding or catalytic cores; includes defined modules such as the C-terminal DMA d... | Long, regional activation over continuous segments (often mid-to-C-terminal), frequently with multiple non-contiguous blocks per protein; avoids structured DNA-binding/catalytic domains; favors low-complexity IDRs with occasional helical substructure. | Interaction site | - DMRT (Doublesex/Mab-3) transcription factors: strong activation over the C-terminal DMA regulatory domain; the N-terminal DM DNA-binding zinc module is not activated.
- Zinc-finger transcription factors (e.g., C2H2 and GATA types, including fungal PacC/Rim101): activation on acidic/Q-/P-/S-rich activation or repressi... | 10,700,821 | 2.967245 | 15.273438 | [
{
"activation": 15.2734375,
"uniref_id": "A0A1E3HAG4"
},
{
"activation": 15.1015625,
"uniref_id": "K0KUE6"
},
{
"activation": 15.09375,
"uniref_id": "A0A1E3IVS5"
},
{
"activation": 15.0390625,
"uniref_id": "A0AAD6KET2"
},
{
"activation": 15,
"uniref_id": "A0A5... | [
{
"activation": 13.898374557495117,
"uniprot_id": "Q9UTM0"
},
{
"activation": 13.638506889343262,
"uniprot_id": "Q2QX40"
},
{
"activation": 13.385465621948242,
"uniprot_id": "Q0WT24"
},
{
"activation": 13.084635734558105,
"uniprot_id": "Q12433"
},
{
"activation": ... | [
6065,
2834,
8740,
14783,
1572,
2614,
6284,
2442,
11199,
5318
] | 0.351868 |
40 | Summary: Alpha-helical transmembrane segments of multi-pass integral membrane proteins—predominantly transporters (permeases, efflux pumps/flippases, ABC/MFS/MATE, nucleotide‑sugar carriers) but also embedded membrane enzymes involved in glyco-/lipid processing (e.g., ArnT, PMTs)—capturing generic TM helices rather tha... | Alpha-helical transmembrane segments of multi-pass integral membrane proteins—predominantly transporters (permeases, efflux pumps/flippases, ABC/MFS/MATE, nucleotide‑sugar carriers) but also embedded membrane enzymes involved in glyco-/lipid processing (e.g., ArnT, PMTs)—capturing generic TM helices rather than substra... | Residue-level peaks confined to multiple transmembrane alpha-helices per protein; strongest on hydrophobic/small polar residues (A, V, I, L, F, M, G, S, T), with occasional peaks at aromatic, Pro, or charged “interface/snorkeling” or re-entrant/periplasmic helical positions; little to no activation in loops or disorder... | Membrane-associated | - Wzx family O-antigen flippase: peaks across several TM helices with an additional peak in a short periplasmic helix connecting TMs.
- General alpha‑glucoside permease (PTS IIC-like): sharp, sparse peaks on single residues within TM helices; none in extra-membranous regions.
- MATE multidrug/toxin efflux transporter: ... | 49,763,480 | 1.430284 | 16.90625 | [
{
"activation": 16.90625,
"uniref_id": "A0A024SEM1"
},
{
"activation": 16.4375,
"uniref_id": "A0A7Y4R5N8"
},
{
"activation": 16.296875,
"uniref_id": "UPI002DD98315"
},
{
"activation": 16.109375,
"uniref_id": "A0A7Y4R6G6"
},
{
"activation": 16,
"uniref_id": "A0... | [
{
"activation": 13.663755416870117,
"uniprot_id": "P77377"
},
{
"activation": 13.644752502441406,
"uniprot_id": "P0AAA7"
},
{
"activation": 13.50279712677002,
"uniprot_id": "P68183"
},
{
"activation": 13.460926055908203,
"uniprot_id": "O32273"
},
{
"activation": 1... | [
4452,
1956,
551,
9383,
9501,
4801,
142,
13969,
15227,
2224
] | 0.439164 |
41 | Summary: Cytochrome P450 (CYP) heme‑thiolate monooxygenases: the feature encodes the P450 catalytic fold and redox apparatus (heme‑binding cysteine motif and surrounding helices) typical of membrane/mitochondrial CYPs across animals, plants, fungi, and bacteria, with occasional weaker cross‑activation on other oxidativ... | Cytochrome P450 (CYP) heme‑thiolate monooxygenases: the feature encodes the P450 catalytic fold and redox apparatus (heme‑binding cysteine motif and surrounding helices) typical of membrane/mitochondrial CYPs across animals, plants, fungi, and bacteria, with occasional weaker cross‑activation on other oxidative metabol... | Predominantly domain‑level activation spanning most of the P450 globular catalytic domain (typical 450–600 aa), with peaks over conserved elements such as the heme‑ligating cysteine motif and K‑helix ExxR region; frequent additional activation on N‑terminal targeting/anchoring segments of microsomal/mitochondrial CYPs.... | Domain | - Drug/xenobiotic‑metabolizing microsomal CYPs (e.g., CYP3A/CYP2 families): broad activation across the catalytic domain including the heme‑binding cysteine region and N‑terminal ER anchor
- Animal steroidogenic CYPs (mitochondrial/ER; e.g., CYP11/17/19/21): strong domain‑level activation plus targeting/anchor segments... | 7,224,914 | 3.36003 | 22.515625 | [
{
"activation": 22.515625,
"uniref_id": "A0A9J5XKK2"
},
{
"activation": 22.28125,
"uniref_id": "A0AAV9EBD1"
},
{
"activation": 21.59375,
"uniref_id": "A0A2S7PYN3"
},
{
"activation": 21.59375,
"uniref_id": "A0A8J2JQF2"
},
{
"activation": 21.578125,
"uniref_id":... | [
{
"activation": 18.777347564697266,
"uniprot_id": "Q6TBX7"
},
{
"activation": 18.71338653564453,
"uniprot_id": "Q93VK5"
},
{
"activation": 18.710779190063477,
"uniprot_id": "Q8N1L4"
},
{
"activation": 18.478954315185547,
"uniprot_id": "Q9LMM1"
},
{
"activation": 1... | [
2672,
7753,
8377,
9256,
3393,
8379,
8165,
9404,
10644,
737
] | 0.261508 |
42 | Summary: Short, highly charged (often acidic) intrinsically disordered tails or linkers—most commonly C‑terminal—that function as linear interaction modules for assembling and regulating large bacterial macromolecular complexes (divisome, ribosome/SRP/translocase, envelope/secretion/motility) and nucleic‑acid–processin... | Short, highly charged (often acidic) intrinsically disordered tails or linkers—most commonly C‑terminal—that function as linear interaction modules for assembling and regulating large bacterial macromolecular complexes (divisome, ribosome/SRP/translocase, envelope/secretion/motility) and nucleic‑acid–processing machine... | Regional activation on one contiguous, tens‑of‑residues charged segment (low hydrophobicity), most often internal or C‑terminal; long multidomain proteins can show multiple independent charged‑IDR segments | Compositional bias | - FtsZ and divisome partners: activation on the short acidic C‑terminal peptide (CTP) used to recruit FtsA/ZipA and many regulators
- SRP receptor FtsY: activation on the acidic, low‑complexity A‑domain/linker adjoining the NG GTPase core
- SecA (protein translocase ATPase): activation on the acidic C‑terminal tail tha... | 35,021,829 | 1.781594 | 13.109375 | [
{
"activation": 13.109375,
"uniref_id": "UPI00279E2586"
},
{
"activation": 13.0234375,
"uniref_id": "A0A921FLR5"
},
{
"activation": 12.9921875,
"uniref_id": "UPI00285B50B6"
},
{
"activation": 12.9609375,
"uniref_id": "A0A841R1Z9"
},
{
"activation": 12.953125,
... | [
{
"activation": 12.259014129638672,
"uniprot_id": "Q8CY87"
},
{
"activation": 11.947818756103516,
"uniprot_id": "F9ULM5"
},
{
"activation": 10.90685749053955,
"uniprot_id": "Q11CP3"
},
{
"activation": 10.611907958984375,
"uniprot_id": "P77173"
},
{
"activation": 1... | [
8686,
12927,
3985,
13990,
9345,
6243,
10744,
8389,
14045,
11584
] | 0.307991 |
43 | Summary: Catalytic cores of transferase enzymes with a strong primary signal for eukaryotic protein kinases (especially tyrosine kinases) and the N-glycosylation oligosaccharyltransferase STT3/AglB/PglB family; the feature also secondarily activates on other small‑group transferases (e.g., Golgi glycosyltransferases, w... | Catalytic cores of transferase enzymes with a strong primary signal for eukaryotic protein kinases (especially tyrosine kinases) and the N-glycosylation oligosaccharyltransferase STT3/AglB/PglB family; the feature also secondarily activates on other small‑group transferases (e.g., Golgi glycosyltransferases, wall teich... | Domain-level activation across catalytic cores with residue-level peaks on hallmark motifs (HRD/DFG/APE and activation‑loop Tyr pairs in kinases; WWDYG in OST). Similar core-focused activation is seen in other transferases (e.g., MT-A70 methyltransferases around the DPPW catalytic region; glyco-/phosphotransferases aro... | Catalytic function | - Receptor and cytosolic tyrosine kinases (EGFR/ErbB, PDGFR/VEGFR/FGFR, insulin receptor; Src/Abl/FAK/JAK): activation across the cytosolic kinase domain with peaks at HRD, DFG, APE, and activation-loop YxY.
- Viral transforming kinases and pseudokinase-like receptors (v-Src/v-Abl; ROR/RYK): fold-level activation of th... | 43,622,473 | 1.561993 | 16.09375 | [
{
"activation": 16.09375,
"uniref_id": "K0FCU0"
},
{
"activation": 15.7421875,
"uniref_id": "UPI00288E8561"
},
{
"activation": 15.5703125,
"uniref_id": "UPI0037F2EEAD"
},
{
"activation": 15.5078125,
"uniref_id": "UPI001F07B497"
},
{
"activation": 15.4921875,
"... | [
{
"activation": 13.828811645507812,
"uniprot_id": "B1MM06"
},
{
"activation": 11.964616775512695,
"uniprot_id": "Q8BJQ9"
},
{
"activation": 11.707620620727539,
"uniprot_id": "Q5DTK1"
},
{
"activation": 10.736298561096191,
"uniprot_id": "Q24488"
},
{
"activation": ... | [
7161,
8294,
791,
8013,
15104,
3872,
8011,
2197,
12058,
891
] | 0.382333 |
44 | Summary: Glycine-rich FAD pyrophosphate–binding loop (GxGxxG) and the adjoining β1–α1–β2 segment of the FAD-binding domain shared across flavoenzymes, including monooxygenases, amine/acid oxidases, flavin-dependent halogenases, FNR-like reductases, and tRNA-modifying flavoenzymes; and, in a minority of cases, structura... | Glycine-rich FAD pyrophosphate–binding loop (GxGxxG) and the adjoining β1–α1–β2 segment of the FAD-binding domain shared across flavoenzymes, including monooxygenases, amine/acid oxidases, flavin-dependent halogenases, FNR-like reductases, and tRNA-modifying flavoenzymes; and, in a minority of cases, structurally analo... | Regional activation over 20–60 residues centered on a small-residue–rich (often di-/tri-gly) phosphate-binding–like loop frequently flanked by basic residues; typically near the N-terminus of PHBH-/FMO-/BVMO-like enzymes, but in mid-chain in many FNR/disulfide reductases; in eukaryotic organelle-targeted proteins, ofte... | Ligand-binding site | - Class A FAD-dependent monooxygenases (p-hydroxybenzoate hydroxylase–like), including flavin-dependent halogenases: activation on the N-terminal GxGxxG loop and adjacent α1 helix that binds FAD’s pyrophosphate
- Baeyer–Villiger monooxygenases (e.g., CHMO-type): activation on the canonical glycine-rich FAD-binding loop... | 9,190,425 | 3.119403 | 25.71875 | [
{
"activation": 25.71875,
"uniref_id": "A0A8J3B4F0"
},
{
"activation": 25.53125,
"uniref_id": "A0A2V4P1A4"
},
{
"activation": 25.453125,
"uniref_id": "UPI0031DB6E87"
},
{
"activation": 25.421875,
"uniref_id": "UPI00054B12A2"
},
{
"activation": 25.40625,
"unire... | [
{
"activation": 23.334749221801758,
"uniprot_id": "Q4WD43"
},
{
"activation": 23.210174560546875,
"uniprot_id": "Q2U5I0"
},
{
"activation": 23.047279357910156,
"uniprot_id": "Q4JA33"
},
{
"activation": 23.04450225830078,
"uniprot_id": "F2JXJ2"
},
{
"activation": 2... | [
8383,
3588,
9024,
14488,
7627,
12522,
5176,
12538,
6026,
5220
] | 1.059644 |
45 | Summary: Extended, non‑catalytic interaction/assembly segments in eukaryotic proteins—typically long, compositionally biased tracts (e.g., FG‑repeat and S/T/P/Q‑rich low‑complexity IDRs) or elongated helical stalk/coiled‑coil regions—used for transport selectivity, transcriptional regulation, or oligomeric assembly rat... | Extended, non‑catalytic interaction/assembly segments in eukaryotic proteins—typically long, compositionally biased tracts (e.g., FG‑repeat and S/T/P/Q‑rich low‑complexity IDRs) or elongated helical stalk/coiled‑coil regions—used for transport selectivity, transcriptional regulation, or oligomeric assembly rather than ... | Predominantly regional activation over very long, contiguous stretches. Strong on IDRs and low‑complexity segments (often Gly/Pro‑rich) and on non‑catalytic helical stalk/coiled‑coil domains; minimal on compact catalytic motifs (e.g., P‑loop/GTP‑binding motifs). In some transcription factors, activation can also cover ... | Interaction site | - Dynamin‑like large GTPases (e.g., Mx/dynamin family): activation across the Middle/Stalk and GED helical assembly domains; not on the N‑terminal P‑loop GTPase motifs.
- E2F/DP transcription factor partners: activation on N‑ and C‑terminal disordered, low‑complexity regulatory tails; absent from the structured central... | 3,882,946 | 3.980971 | 21.171875 | [
{
"activation": 21.171875,
"uniref_id": "UPI001AE36E0E"
},
{
"activation": 21.09375,
"uniref_id": "A0A8B8IS98"
},
{
"activation": 21.078125,
"uniref_id": "A0AAV3YLZ3"
},
{
"activation": 21.0625,
"uniref_id": "A0A1S3JPJ6"
},
{
"activation": 21.03125,
"uniref_id... | [
{
"activation": 20.394380569458008,
"uniprot_id": "Q9Y4C4"
},
{
"activation": 20.271799087524414,
"uniprot_id": "Q3UHC2"
},
{
"activation": 19.92026138305664,
"uniprot_id": "Q54M77"
},
{
"activation": 19.734697341918945,
"uniprot_id": "Q9FJ57"
},
{
"activation": 1... | [
9673,
3064,
9899,
6542,
3071,
10930,
7799,
13315,
5944,
3840
] | 0.199277 |
46 | Summary: Long, charge-dense, low‑complexity tracts (Lys/Arg- and Glu/Asp-rich) that are typically intrinsically disordered tails or extended coiled‑coil segments used as electrostatic interaction modules for DNA/RNA engagement and assembly of large macromolecular complexes; the feature also fires on analogous charged a... | Long, charge-dense, low‑complexity tracts (Lys/Arg- and Glu/Asp-rich) that are typically intrinsically disordered tails or extended coiled‑coil segments used as electrostatic interaction modules for DNA/RNA engagement and assembly of large macromolecular complexes; the feature also fires on analogous charged accessory ... | Long regional activation along contiguous segments, frequently beginning at the N‑terminus; strong preference for polyampholytic IDRs and heptad‑repeat coiled‑coils; little or no activation in compact globular catalytic cores. In histones, activation concentrates on the N‑terminal tail; in SMC/kleisin systems, on exten... | Compositional bias | - Cohesin/condensin/SMC system (SMC1/SMC3/Smc, kleisins RAD21/REC8, ScpA/ScpB): activation along long charged coiled‑coil arms and linker regions that scaffold DNA and mediate subunit interactions.
- Histone H3 and centromeric H3 (CENP‑A/Cse4): activation on the Lys‑rich, intrinsically disordered N‑terminal tail; minim... | 3,881,452 | 3.981356 | 22.640625 | [
{
"activation": 22.640625,
"uniref_id": "A0A925ELP5"
},
{
"activation": 22.609375,
"uniref_id": "A0A7J2JRK1"
},
{
"activation": 22.5,
"uniref_id": "A0A7J2JQT9"
},
{
"activation": 22.453125,
"uniref_id": "A0A8T4UNB7"
},
{
"activation": 22.390625,
"uniref_id": "... | [
{
"activation": 19.161487579345703,
"uniprot_id": "P75478"
},
{
"activation": 17.90043067932129,
"uniprot_id": "P35154"
},
{
"activation": 16.446836471557617,
"uniprot_id": "Q6BDR8"
},
{
"activation": 16.02914810180664,
"uniprot_id": "Q3V124"
},
{
"activation": 15... | [
4331,
2795,
12706,
941,
5742,
5382,
1898,
2161,
7539,
6948
] | 0.21118 |
47 | Summary: Alpha-helical transmembrane segments and their helix–helix packing motifs (e.g., GxxxG/AxxxA) in multi-pass membrane proteins, especially in bioenergetic and transporter complexes; the feature also accentuates embedded polar catalytic/gating residues within these helices.
Activation pattern: Primarily regional... | Alpha-helical transmembrane segments and their helix–helix packing motifs (e.g., GxxxG/AxxxA) in multi-pass membrane proteins, especially in bioenergetic and transporter complexes; the feature also accentuates embedded polar catalytic/gating residues within these helices. | Primarily regional activation across long, contiguous hydrophobic alpha-helices (entire TM segments, often consecutively), with occasional residue-level peaks at embedded polar or small-residue motifs; weaker spillover onto long non-membrane alpha-helices/coiled-coils. | Membrane-associated | - Light-harvesting antenna and photosystem thylakoid proteins: strong activation along multi-pass transmembrane helices and small-residue packing motifs in chloroplast-targeted membrane subunits.
- Formate–nitrite (FNT) family channels: activation across the six-helix bundle and at gly/small-residue constriction/packin... | 4,309,412 | 3.876764 | 14.859375 | [
{
"activation": 14.859375,
"uniref_id": "UPI00289AB384"
},
{
"activation": 14.8359375,
"uniref_id": "A0A087NGR5"
},
{
"activation": 14.7890625,
"uniref_id": "UPI00391F1D5D"
},
{
"activation": 14.7265625,
"uniref_id": "UPI001B3CE905"
},
{
"activation": 14.71875,
... | [
{
"activation": 12.308520317077637,
"uniprot_id": "Q02113"
},
{
"activation": 12.10859203338623,
"uniprot_id": "Q02114"
},
{
"activation": 11.499820709228516,
"uniprot_id": "P27521"
},
{
"activation": 11.33534049987793,
"uniprot_id": "Q9SY97"
},
{
"activation": 11... | [
12221,
4201,
10137,
9663,
8711,
7050,
7371,
1385,
4122,
920
] | 0.295263 |
48 | Summary: Extended, low-complexity intrinsically disordered regions enriched in serine, threonine, proline (with frequent glycine and charged residues), typically found in C-terminal tails and inter-domain linkers of large eukaryotic regulatory proteins (not the folded domains themselves); common in ubiquitin-system ada... | Extended, low-complexity intrinsically disordered regions enriched in serine, threonine, proline (with frequent glycine and charged residues), typically found in C-terminal tails and inter-domain linkers of large eukaryotic regulatory proteins (not the folded domains themselves); common in ubiquitin-system adaptors (UB... | Regional activation across long disordered stretches with low predicted structure; strong bias toward high sequence indices (often C-termini) and large proteins; minimal signal in well-folded domains; occasional residue-level peak at the extreme C-terminus that can overlap the boundary of a structured C-terminal domain | Disorder | - UBX domain–containing p97/VCP adaptor proteins: activation on long, disordered N- or mid-region linkers; no activation on the UBX fold.
- Nuclear RNA export factor (NXF1/TAP family): activation over a disordered C-terminal segment with a terminal peak at the last residues, extending into the TAP-C/UBA-like C-terminal... | 7,105,269 | 3.376729 | 23.25 | [
{
"activation": 23.25,
"uniref_id": "A0A423TTK4"
},
{
"activation": 22.96875,
"uniref_id": "UPI001EF8B3AA"
},
{
"activation": 22.953125,
"uniref_id": "UPI001E277273"
},
{
"activation": 22.9375,
"uniref_id": "A0A9P0F243"
},
{
"activation": 22.921875,
"uniref_id... | [
{
"activation": 21.77630043029785,
"uniprot_id": "Q8MJ87"
},
{
"activation": 20.835073471069336,
"uniprot_id": "P54729"
},
{
"activation": 19.929445266723633,
"uniprot_id": "Q05610"
},
{
"activation": 19.01382827758789,
"uniprot_id": "Q9LYE5"
},
{
"activation": 18... | [
9619,
10221,
5062,
1319,
12153,
4247,
8348,
3494,
1638,
4609
] | 0.222565 |
49 | Summary: Conserved hydrophobic/aromatic packing and binding residues within compact polymer-binding modules—most prominently helix–turn–helix/homeodomain DNA-binding folds and LysM peptidoglycan-binding repeats—with similar signals in cell-wall–binding repeats and in substrate-binding surfaces of secreted metalloprotea... | Conserved hydrophobic/aromatic packing and binding residues within compact polymer-binding modules—most prominently helix–turn–helix/homeodomain DNA-binding folds and LysM peptidoglycan-binding repeats—with similar signals in cell-wall–binding repeats and in substrate-binding surfaces of secreted metalloproteases; seco... | Residue-level peaks clustered within a small domain (typically 40–70 aa), enriched for hydrophobic/aromatic residues (Leu, Ile, Val, Ala, Phe, Trp, Tyr). In bacterial HTH/phage repressors and antitoxins, peaks occur near the N-terminus (often around positions 20–40, including a conserved Trp). In eukaryotic transcripti... | Ligand-binding site | - Bacterial HTH repressors (XRE/CI/Cro/MarR/TetR families): activation on hydrophobic/aromatic residues in the N-terminal HTH bundle that pack the helices and aid DNA recognition/dimerization.
- Type II toxin–antitoxin antitoxins (HTH DNA-binding proteins): activation on core hydrophobics that stabilize the HTH used fo... | 35,959,786 | 1.755164 | 26.15625 | [
{
"activation": 26.15625,
"uniref_id": "A0A948S765"
},
{
"activation": 26.078125,
"uniref_id": "UPI00141A6F60"
},
{
"activation": 25.875,
"uniref_id": "A0A2P1N3C5"
},
{
"activation": 25.796875,
"uniref_id": "UPI0037C64B3A"
},
{
"activation": 25.765625,
"uniref... | [
{
"activation": 24.135040283203125,
"uniprot_id": "H9C180"
},
{
"activation": 23.778993606567383,
"uniprot_id": "P11408"
},
{
"activation": 22.099143981933594,
"uniprot_id": "Q56185"
},
{
"activation": 21.68476676940918,
"uniprot_id": "Q8EIX4"
},
{
"activation": 2... | [
7148,
6231,
12409,
15573,
14481,
12025,
11363,
13528,
12187,
3484
] | 0.246035 |
50 | Summary: Asparagine-biased alpha-helical interface segments—primarily coiled‑coil/dimerization helices (heptad a/d ILV plus a key Asn) used for signal transmission or subunit docking—most prominently the transmitter helices of two‑component/hybrid histidine kinases (DHp/HAMP‑like), but also analogous N‑terminal assembl... | Asparagine-biased alpha-helical interface segments—primarily coiled‑coil/dimerization helices (heptad a/d ILV plus a key Asn) used for signal transmission or subunit docking—most prominently the transmitter helices of two‑component/hybrid histidine kinases (DHp/HAMP‑like), but also analogous N‑terminal assembly/docking... | Regional activation along long alpha‑helical stretches with heptad-repeat signatures: peaks on I/L/V at a/d positions and on interfacial Asn. In histidine kinases this occurs in mid‑to‑late sequence (often yielding spaced I–N–L triads). In tRNA‑splicing endonucleases it clusters at the extreme N‑terminus. Short, strong... | Structural motif | - Two‑component/hybrid histidine kinases (ion, envelope‑stress, virulence, phosphate regulators; plant/fungal osmosensors/phytohormone HKs; cyanobacterial circadian HKs): activation on DHp/HAMP‑like coiled‑coil transmitter helices, highlighting ILV heptad positions and interfacial Asn (e.g., repeated I–N–L spacing in D... | 14,000,357 | 2.698483 | 13.265625 | [
{
"activation": 13.265625,
"uniref_id": "A0A7C3RJR8"
},
{
"activation": 13.015625,
"uniref_id": "A0A933B019"
},
{
"activation": 13.015625,
"uniref_id": "A0A1Q9N1C4"
},
{
"activation": 13.0078125,
"uniref_id": "A0A7C4C3V6"
},
{
"activation": 13,
"uniref_id": "A... | [
{
"activation": 9.103902816772461,
"uniprot_id": "P37894"
},
{
"activation": 9.067481994628906,
"uniprot_id": "P0AEC6"
},
{
"activation": 9.013860702514648,
"uniprot_id": "P0AEC3"
},
{
"activation": 9.011235237121582,
"uniprot_id": "Q54RP6"
},
{
"activation": 8.97... | [
4696,
3299,
13197,
14548,
13156,
2309,
7478,
13736,
8588,
11174
] | 0.386618 |
51 | Summary: Short, charged, low‑complexity patches (5–15 aa) in intrinsically disordered tails/loops—typically polybasic or mixed‑charge and often glycine‑flanked—that serve as generic electrostatic interaction motifs (e.g., nucleic‑acid binding, binding acidic peptide tails such as tubulin E‑hooks, or modular protein–pro... | Short, charged, low‑complexity patches (5–15 aa) in intrinsically disordered tails/loops—typically polybasic or mixed‑charge and often glycine‑flanked—that serve as generic electrostatic interaction motifs (e.g., nucleic‑acid binding, binding acidic peptide tails such as tubulin E‑hooks, or modular protein–protein dock... | Residue-level peaks concentrated in multiple short clusters (≈5–15 aa) per protein, frequently in disordered linkers, N/C‑terminal tails, or exposed loops; observed in both soluble and membrane‑associated proteins | Compositional bias | - Ribosomal proteins/translation factors: K/R‑rich rRNA‑binding tails and basic surface loops.
- DNA topoisomerases/gyrases: short K/R‑enriched DNA‑contacting loops near catalytic cores.
- Bacteriophage major capsid proteins: arginine/lysine‑rich N‑terminal genome‑binding arms.
- Tubulin‑tyrosine ligase–like (TTLL/TTL)... | 92,820,794 | 0.806895 | 12.851563 | [
{
"activation": 12.8515625,
"uniref_id": "UPI0035B1B149"
},
{
"activation": 12.59375,
"uniref_id": "UPI0013F167BA"
},
{
"activation": 12.5859375,
"uniref_id": "A0A249XS59"
},
{
"activation": 12.4921875,
"uniref_id": "A0A7C7P5A6"
},
{
"activation": 12.453125,
"... | [
{
"activation": 11.482416152954102,
"uniprot_id": "P21464"
},
{
"activation": 11.311766624450684,
"uniprot_id": "Q9D3A8"
},
{
"activation": 11.201216697692871,
"uniprot_id": "D3ZAR1"
},
{
"activation": 11.028117179870605,
"uniprot_id": "Q8STF6"
},
{
"activation": ... | [
2192,
14779,
7857,
15296,
15331,
10484,
9265,
697,
5900,
15227
] | 0.24087 |
52 | Summary: ABC ATPase nucleotide‑binding domain signature region: the C‑loop/LSGGQ motif and adjacent switch elements (Q‑loop/H‑loop/D‑loop) immediately downstream of the Walker A P‑loop, which form the interfacial catalytic site and couple ATP binding/hydrolysis to dimerization and transport (or analogous actions in ABC... | ABC ATPase nucleotide‑binding domain signature region: the C‑loop/LSGGQ motif and adjacent switch elements (Q‑loop/H‑loop/D‑loop) immediately downstream of the Walker A P‑loop, which form the interfacial catalytic site and couple ATP binding/hydrolysis to dimerization and transport (or analogous actions in ABC‑like ATP... | Regional activation within each NBD as long, contiguous blocks just C‑terminal to the Walker A motif; often two separate activated regions in full‑length transporters with two NBDs and a single region in half‑transporters. Peaks commonly fall over Gly/Gly of the LSGGQ signature or nearby G/K/T‑rich segments (including ... | Catalytic function | - Metazoan full‑length ABC transporters (e.g., ABCA/ABCD): activation in both NBDs immediately C‑terminal to Walker A, spanning the C‑loop/switch region (as in CED‑7).
- Peroxisomal very‑long‑chain fatty‑acid importers (ABCD family): activation on the loop between Walker A and Walker B encompassing the C‑loop/Q‑loop se... | 6,655,668 | 3.442096 | 23.390625 | [
{
"activation": 23.390625,
"uniref_id": "A0A7W2YHE1"
},
{
"activation": 23.1875,
"uniref_id": "UPI00138DF2C4"
},
{
"activation": 23.046875,
"uniref_id": "UPI00295EA789"
},
{
"activation": 23.03125,
"uniref_id": "UPI0028061A76"
},
{
"activation": 23.015625,
"un... | [
{
"activation": 22.170700073242188,
"uniprot_id": "P31060"
},
{
"activation": 21.683008193969727,
"uniprot_id": "Q9LZ98"
},
{
"activation": 21.424339294433594,
"uniprot_id": "P16521"
},
{
"activation": 21.321128845214844,
"uniprot_id": "P0DX93"
},
{
"activation": ... | [
2480,
4515,
1219,
14668,
6256,
5023,
4967,
7704,
3093,
2897
] | 0.247095 |
53 | Summary: Divalent metal–dependent phosphate-bond–processing catalytic cores, especially two‑metal‑ion Mg2+/Mn2+ enzymes that act on nucleotides or nucleic acids (phosphodiesterases and nucleases), with weaker cross‑activation of related nucleotide transferases and other His‑rich metalloenzymes
Activation pattern: Domai... | Divalent metal–dependent phosphate-bond–processing catalytic cores, especially two‑metal‑ion Mg2+/Mn2+ enzymes that act on nucleotides or nucleic acids (phosphodiesterases and nucleases), with weaker cross‑activation of related nucleotide transferases and other His‑rich metalloenzymes | Domain-level activation over long, contiguous segments that align with the structured catalytic core (helices/loops around the metal site) rather than a short motif; enriched in acidic/His residues; often tracks the scaffold surrounding the active site and may spare the exact catalytic residues; strongest in soluble en... | Domain | - Class I 3′,5′‑cyclic‑nucleotide phosphodiesterases (PDEase domain): activation across the C‑terminal catalytic core helices/loops rather than the specific H/D metal‑binding residues
- Bacterial cyclic‑di‑nucleotide phosphodiesterases (EAL and HD‑GYP families): activation over the metal‑binding catalytic domain that h... | 5,144,123 | 3.69971 | 26.953125 | [
{
"activation": 26.953125,
"uniref_id": "UPI0006A69E97"
},
{
"activation": 26.515625,
"uniref_id": "A0A420Z9D1"
},
{
"activation": 26.40625,
"uniref_id": "A0AAN5KRD6"
},
{
"activation": 26.359375,
"uniref_id": "A0A955LA44"
},
{
"activation": 26.296875,
"uniref... | [
{
"activation": 23.874614715576172,
"uniprot_id": "Q5ZTK4"
},
{
"activation": 20.92755126953125,
"uniprot_id": "P06776"
},
{
"activation": 20.31819725036621,
"uniprot_id": "Q8IKD3"
},
{
"activation": 20.313846588134766,
"uniprot_id": "P22143"
},
{
"activation": 20... | [
11128,
12619,
1102,
9765,
2385,
11420,
2646,
8007,
2229,
1797
] | 0.146239 |
54 | Summary: Acidic phosphotransfer microenvironment of two‑component signaling modules—specifically the Asp-centered active site region of receiver (REC) domains and the H‑box/DHp region of histidine kinases—defined by clustered Asp/Glu with nearby Ser/Thr and Lys/Arg in α‑helix/loop contexts; occasionally flags similar a... | Acidic phosphotransfer microenvironment of two‑component signaling modules—specifically the Asp-centered active site region of receiver (REC) domains and the H‑box/DHp region of histidine kinases—defined by clustered Asp/Glu with nearby Ser/Thr and Lys/Arg in α‑helix/loop contexts; occasionally flags similar acidic α‑h... | Residue-level peaks in small clusters (≈3–6 residues): (1) N‑terminal segment of response regulators’ REC domains (≈positions 10–45 of the protein) around the phosphorylatable Asp and neighboring acidic/Ser/Thr/Lys residues; (2) internal cytosolic H‑box within the DHp helix of histidine kinases, sometimes including the... | Catalytic function | - Response regulators (CheY-/OmpR/PhoB‑like): peaks over the REC active-site pocket (phosphorylatable Asp, metal‑binding acidic residues, nearby Ser/Thr/Lys switch positions)
- Sensor and hybrid histidine kinases (HisKA/DHp + CA): peaks around the H‑box within the DHp coiled‑coil that harbors the autophosphorylation Hi... | 38,781,771 | 1.679615 | 25.234375 | [
{
"activation": 25.234375,
"uniref_id": "UPI0034D673F3"
},
{
"activation": 25.171875,
"uniref_id": "A0A0F9EFI7"
},
{
"activation": 25,
"uniref_id": "A0A0S4L8L9"
},
{
"activation": 24.890625,
"uniref_id": "A0A928UDG3"
},
{
"activation": 24.875,
"uniref_id": "A0... | [
{
"activation": 22.955350875854492,
"uniprot_id": "P06628"
},
{
"activation": 22.33010482788086,
"uniprot_id": "P40138"
},
{
"activation": 21.7404727935791,
"uniprot_id": "P46384"
},
{
"activation": 21.56619644165039,
"uniprot_id": "P0AE67"
},
{
"activation": 21.4... | [
15486,
8603,
7754,
9778,
9021,
14644,
764,
6551,
7950,
9289
] | 0.211645 |
55 | Summary: Acidic/polar, glycine-rich intrinsically disordered N-terminal extensions of enzymes—low-complexity tails that precede the folded catalytic/core domains across diverse metabolic enzyme families and multi-subunit complexes
Activation pattern: Regional activation concentrated in long N-terminal stretches (typica... | Acidic/polar, glycine-rich intrinsically disordered N-terminal extensions of enzymes—low-complexity tails that precede the folded catalytic/core domains across diverse metabolic enzyme families and multi-subunit complexes | Regional activation concentrated in long N-terminal stretches (typically first 20–80 residues), with diffuse peaks across many positions in low-pLDDT, coil-rich segments; little to no activation in the well-structured catalytic domains; occasionally also on analogous flexible linkers/tails in different subunits | Disorder | - B12-dependent propanediol dehydratase (large subunit): strong activation on the disordered N-terminal tail (~1–60 aa), not the structured catalytic core.
- Glutamine synthetase: activation confined to the unstructured N-terminus (~2–40 aa), absent from the GS beta-grasp and catalytic domains.
- UbiD/prFMN aromatic ac... | 10,869,192 | 2.951633 | 22.171875 | [
{
"activation": 22.171875,
"uniref_id": "A0A2G6QSG2"
},
{
"activation": 22.140625,
"uniref_id": "UPI0021F3A6DE"
},
{
"activation": 22.125,
"uniref_id": "UPI0031BB8FF5"
},
{
"activation": 22.078125,
"uniref_id": "A0A7X7JU74"
},
{
"activation": 22.078125,
"unire... | [
{
"activation": 21.671401977539062,
"uniprot_id": "P11652"
},
{
"activation": 19.626955032348633,
"uniprot_id": "P22033"
},
{
"activation": 19.576427459716797,
"uniprot_id": "Q59676"
},
{
"activation": 18.152240753173828,
"uniprot_id": "I3VE77"
},
{
"activation": ... | [
15236,
4545,
106,
4138,
2665,
9795,
14698,
3370,
11198,
407
] | 0.282212 |
56 | Summary: Conserved beta-strand immediately N-terminal to the Walker A (P-loop) within ABC nucleotide-binding domains (NBDs)—the beta1/A-loop region that positions ATP’s adenine and precedes the P-loop—consistently recognized across diverse ABC transporters and ABC-like ATPases.
Activation pattern: Predominantly residue... | Conserved beta-strand immediately N-terminal to the Walker A (P-loop) within ABC nucleotide-binding domains (NBDs)—the beta1/A-loop region that positions ATP’s adenine and precedes the P-loop—consistently recognized across diverse ABC transporters and ABC-like ATPases. | Predominantly residue-level peaks within short 6–12 aa windows, centered on a hydrophobic residue in a structured beta-strand; positioned ~15–25 residues upstream of the Walker A motif. In large eukaryotic ABCs with two NBDs, two mirrored peaks occur at the equivalent site in each NBD. The site lies near the N-terminus... | Ligand-binding site | - ECF transporter A components (EcfA/EcfA1, bacterial importers): single sharp peak on the N-terminal beta1 strand immediately before the Walker A loop.
- ABCA family lipid exporters (e.g., ABCA3): two symmetric peaks, each ~20 residues N-terminal to the respective Walker A in the tandem NBDs.
- ABCB mitochondrial pept... | 19,139,025 | 2.385836 | 30.875 | [
{
"activation": 30.875,
"uniref_id": "A0A3Q9RJE2"
},
{
"activation": 30.65625,
"uniref_id": "A0A6B3FNA9"
},
{
"activation": 30.625,
"uniref_id": "UPI0031E8A567"
},
{
"activation": 30.59375,
"uniref_id": "UPI00398A975F"
},
{
"activation": 30.515625,
"uniref_id"... | [
{
"activation": 27.846668243408203,
"uniprot_id": "Q8LEF6"
},
{
"activation": 27.841983795166016,
"uniprot_id": "P54683"
},
{
"activation": 27.7906436920166,
"uniprot_id": "Q9AT00"
},
{
"activation": 27.78575897216797,
"uniprot_id": "P9WQJ9"
},
{
"activation": 27.... | [
10937,
308,
2555,
9995,
1724,
1291,
2120,
313,
5816,
5772
] | 0.181767 |
57 | Summary: Exposed, tryptophan/tyrosine-rich binding patches on extracellular proteins that engage cell-surface glycans, glycolipids, and selected membrane lipid headgroups; prominently R-type/CBM13 β-trefoil lectins but not exclusive; also present on non-β-trefoil W-rich binding modules and on β-trefoil cytokines with s... | Exposed, tryptophan/tyrosine-rich binding patches on extracellular proteins that engage cell-surface glycans, glycolipids, and selected membrane lipid headgroups; prominently R-type/CBM13 β-trefoil lectins but not exclusive; also present on non-β-trefoil W-rich binding modules and on β-trefoil cytokines with similar lo... | Patch/domain-level activation on solvent-exposed, flexible loops enriched in Trp/Tyr plus polar residues; strongest on R-type/CBM13 lectins; also observed on non-β-trefoil W-rich surfaces that bind glycans, glycosphingolipids, or sphingomyelin/phosphocholine headgroups; in multi-domain proteins, confined to these patch... | Ligand-binding site | - R-type (ricin B–like) lectin domains in secreted receptors (e.g., MRC/Endo180, sPLA2R): strong W-centered peaks in the N-terminal ricin-like lectin; no activation on downstream CTLD or Fn-II.
- Polypeptide GalNAc-transferases: activation on the C-terminal ricin-like lectin domain, not the catalytic transferase core.
... | 9,438,464 | 3.092772 | 24.796875 | [
{
"activation": 24.796875,
"uniref_id": "UPI00056B7242"
},
{
"activation": 24.6875,
"uniref_id": "UPI0025D63BE5"
},
{
"activation": 24.6875,
"uniref_id": "UPI002953FB1C"
},
{
"activation": 24.671875,
"uniref_id": "UPI00342E8A96"
},
{
"activation": 24.578125,
"... | [
{
"activation": 22.929811477661133,
"uniprot_id": "Q9NZH7"
},
{
"activation": 22.918235778808594,
"uniprot_id": "O06522"
},
{
"activation": 22.786460876464844,
"uniprot_id": "P28586"
},
{
"activation": 22.71554183959961,
"uniprot_id": "Q46668"
},
{
"activation": 2... | [
13975,
9470,
10409,
639,
6494,
6701,
9373,
12195,
10859,
9323
] | 0.18574 |
58 | Summary: Eukaryotic modular interaction domains in large regulatory/scaffold proteins; the feature marks compact, folded binding modules (e.g., BRCT, RRM/UHM, PH, PDZ, SH3, guanylate kinase-like, homeobox/Myb/SANT) that mediate protein–protein, protein–nucleic acid (RNA or DNA), or protein–lipid interactions
Activation... | Eukaryotic modular interaction domains in large regulatory/scaffold proteins; the feature marks compact, folded binding modules (e.g., BRCT, RRM/UHM, PH, PDZ, SH3, guanylate kinase-like, homeobox/Myb/SANT) that mediate protein–protein, protein–nucleic acid (RNA or DNA), or protein–lipid interactions | Domain-level activation over long, contiguous stretches that align with annotated globular domains; signal is distributed across multiple secondary-structure elements within the domain core, with little to no activation in disordered linkers; often selects one copy within tandem repeats (e.g., RRM2 or PDZ2) rather than... | Domain | - RNA polymerase II CTD phosphatases (FCP1/CPL): strong activation across the BRCT interaction domain
- Cytoplasmic polyadenylation element-binding proteins (CPEB): activation confined to the second RRM domain
- TORC2/Sin1 subunits: activation of the C-terminal PH lipid-binding domain
- MAGUK/ZO tight junction scaffold... | 20,943,326 | 2.295745 | 10.453125 | [
{
"activation": 10.453125,
"uniref_id": "A0A7N9AKH2"
},
{
"activation": 10.3984375,
"uniref_id": "A0A978VQK5"
},
{
"activation": 10.3828125,
"uniref_id": "A0A3Q3BXL6"
},
{
"activation": 10.359375,
"uniref_id": "A0A668U968"
},
{
"activation": 10.3515625,
"unire... | [
{
"activation": 10.097003936767578,
"uniprot_id": "Q9Y5B0"
},
{
"activation": 9.634562492370605,
"uniprot_id": "F1LQ48"
},
{
"activation": 9.578816413879395,
"uniprot_id": "Q9Z103"
},
{
"activation": 9.57404899597168,
"uniprot_id": "Q9P376"
},
{
"activation": 9.53... | [
11394,
7862,
4865,
14686,
13994,
3045,
10746,
7221,
10011,
7661
] | 0.329963 |
59 | Summary: Conserved catalytic-core motifs of eukaryotic protein kinase domains, centered on the activation segment (DFG→activation loop→APE) and adjacent catalytic loop (HRD/LKPEN), which shape substrate positioning and activation-loop regulation, across Ser/Thr, Tyr, and dual-specificity kinases
Activation pattern: Reg... | Conserved catalytic-core motifs of eukaryotic protein kinase domains, centered on the activation segment (DFG→activation loop→APE) and adjacent catalytic loop (HRD/LKPEN), which shape substrate positioning and activation-loop regulation, across Ser/Thr, Tyr, and dual-specificity kinases | Regional activation confined to the protein kinase catalytic domain, with sharp residue-level peaks on short coil segments between β-strands (activation loop/P+1 loop around the APE motif) and additional peaks at nearby conserved motifs (DFG, HRD); typically 8–15 residues per protein within the kinase domain, often at ... | Catalytic function | - AGC-family kinases (e.g., GRKs/PKA/PKC-like): strong activation on the activation loop/P+1 region around the APE motif and adjacent catalytic loop; in rhodopsin kinase GRK1, the peak lies in the coil between β-strands near the APE region with minor peaks elsewhere in the kinase domain.
- MAPK cascade kinases (MAP2Ks ... | 45,536,019 | 1.519062 | 14.726563 | [
{
"activation": 14.7265625,
"uniref_id": "A0A6P7SLT4"
},
{
"activation": 14.6640625,
"uniref_id": "A0A7S1GS61"
},
{
"activation": 14.625,
"uniref_id": "UPI003A7F1AC7"
},
{
"activation": 14.5625,
"uniref_id": "A0AAV2HGY3"
},
{
"activation": 14.5390625,
"uniref_... | [
{
"activation": 13.844671249389648,
"uniprot_id": "Q9GRT1"
},
{
"activation": 13.81289291381836,
"uniprot_id": "Q54IP4"
},
{
"activation": 13.63870620727539,
"uniprot_id": "Q54TH6"
},
{
"activation": 13.623788833618164,
"uniprot_id": "Q54U31"
},
{
"activation": 13... | [
11164,
4310,
15195,
3910,
13226,
13614,
11952,
10495,
8015,
2265
] | 0.545117 |
60 | Summary: Charge-dense, extended regions (long alpha-helical bundles/coiled-coils and/or intrinsically disordered low-complexity segments) that provide broad electrostatic interaction surfaces for nucleic acids and for assembly of large macromolecular complexes; prevalent in very large nucleic-acid enzymes and defense/h... | Charge-dense, extended regions (long alpha-helical bundles/coiled-coils and/or intrinsically disordered low-complexity segments) that provide broad electrostatic interaction surfaces for nucleic acids and for assembly of large macromolecular complexes; prevalent in very large nucleic-acid enzymes and defense/host–patho... | Regional activation over long, continuous stretches (tens–hundreds of residues) enriched in Lys/Arg/Asp/Glu, aligning to extended helices/coiled-coils or charged IDRs rather than sharp catalytic motifs | Compositional bias | - CRISPR-Cas9 nucleases: activation across the helical REC lobe and adjacent RNA:DNA-binding surfaces.
- Viral RNA-dependent RNA polymerases: activation on long charged N/C-terminal regions and basic/acidic flanks surrounding the polymerase core that engage RNA and cofactors.
- Helicases and nucleases: activation on ex... | 10,370,017 | 2.998646 | 11.078125 | [
{
"activation": 11.078125,
"uniref_id": "UPI00259F814C"
},
{
"activation": 10.734375,
"uniref_id": "UPI0032D98FEB"
},
{
"activation": 10.7265625,
"uniref_id": "UPI0031BAC4B1"
},
{
"activation": 10.6953125,
"uniref_id": "UPI00119C919B"
},
{
"activation": 10.6875,
... | [
{
"activation": 10.203250885009766,
"uniprot_id": "Q99ZW2"
},
{
"activation": 9.983174324035645,
"uniprot_id": "A0Q5Y3"
},
{
"activation": 9.820297241210938,
"uniprot_id": "Q6PI26"
},
{
"activation": 9.804780960083008,
"uniprot_id": "Q6LA53"
},
{
"activation": 9.7... | [
4603,
12088,
12123,
9756,
13981,
11177,
9450,
79,
4218,
11744
] | 0.465925 |
61 | Summary: Acidic, serine/threonine- and proline/glycine-rich intrinsically disordered low-complexity regions—often N‑terminal—that serve as transcriptional activation segments or acidic regulatory IDRs in nuclear DNA/RNA regulatory proteins (including transposase‑derived plant TFs, BED zinc‑finger proteins, chromatin fa... | Acidic, serine/threonine- and proline/glycine-rich intrinsically disordered low-complexity regions—often N‑terminal—that serve as transcriptional activation segments or acidic regulatory IDRs in nuclear DNA/RNA regulatory proteins (including transposase‑derived plant TFs, BED zinc‑finger proteins, chromatin factors) an... | Regional activation across long low-complexity tracts with strong residue-level peaks on E/D (often interspersed with S/T and P/G); minimal activation within folded domains; frequently concentrated at the N-terminus | Compositional bias | - Plant FAR1/FRS/FHY3 transcription factors (Mutator-like transposase–derived): activation on the N-terminal disordered acidic segment upstream of FAR1/MULE/SWIM domains.
- Plant B3/REM/VRN1 and AP2/B3 (RAV1) transcription factors: activation on acidic, S/T-rich disordered N-termini that function as activation domains.... | 8,108,147 | 3.244696 | 22.234375 | [
{
"activation": 22.234375,
"uniref_id": "UPI00227D64B2"
},
{
"activation": 22.1875,
"uniref_id": "A0A445CTU2"
},
{
"activation": 22.125,
"uniref_id": "A0A7J9APJ2"
},
{
"activation": 22.125,
"uniref_id": "A0A445EAR3"
},
{
"activation": 22.109375,
"uniref_id": "... | [
{
"activation": 15.75932502746582,
"uniprot_id": "Q9LIE5"
},
{
"activation": 12.466460227966309,
"uniprot_id": "Q9SY66"
},
{
"activation": 11.272061347961426,
"uniprot_id": "Q17604"
},
{
"activation": 9.192255020141602,
"uniprot_id": "B9FJG3"
},
{
"activation": 8.... | [
13120,
3384,
6870,
16146,
4524,
6740,
2395,
5558,
16242,
13151
] | 0.233293 |
62 | Summary: Extracellular/luminal re-entrant pore-loop and selectivity-filter region at the outer mouth of the permeation pathway, spanning the pore helix/loop between adjacent transmembrane helices (e.g., S5–pore–S6 or equivalent) in multi-pass ion channels and analogous pore-facing re-entrant segments in transporters
Ac... | Extracellular/luminal re-entrant pore-loop and selectivity-filter region at the outer mouth of the permeation pathway, spanning the pore helix/loop between adjacent transmembrane helices (e.g., S5–pore–S6 or equivalent) in multi-pass ion channels and analogous pore-facing re-entrant segments in transporters | Regional activation in long, contiguous stretches that start at the end of the preceding TM helix, peak across the pore helix/selectivity-filter and adjacent extracellular “turret/vestibule,” and taper into the following TM helix; repeated blocks in multidomain channels (e.g., four repeats in Nav); enrichment for hydro... | Structural motif | - TRP channels (TRPM/TRPML and others): strong activation on the S5–pore helix/loop–S6 region including the selectivity filter and disulfide-bonded residues shaping ion permeability.
- Voltage-gated sodium channels (Nav): activation on each domain’s P-loop/turret at the extracellular pore mouth between S5 and S6, dropp... | 8,991,445 | 3.141292 | 17.59375 | [
{
"activation": 17.59375,
"uniref_id": "A0AAD6PML9"
},
{
"activation": 17.5625,
"uniref_id": "A0A978VVR6"
},
{
"activation": 17.46875,
"uniref_id": "K0SEN3"
},
{
"activation": 17.453125,
"uniref_id": "A0AAV2H6P6"
},
{
"activation": 17.40625,
"uniref_id": "A0A4... | [
{
"activation": 16.394573211669922,
"uniprot_id": "Q9C0Y5"
},
{
"activation": 16.23813819885254,
"uniprot_id": "Q84TH5"
},
{
"activation": 16.21877670288086,
"uniprot_id": "Q9NGP5"
},
{
"activation": 16.18160057067871,
"uniprot_id": "P33302"
},
{
"activation": 16.... | [
13602,
2478,
756,
4651,
9908,
14112,
15967,
12835,
11557,
3328
] | 0.30802 |
63 | Summary: Acidic low‑complexity intrinsically disordered segments, most often C‑terminal tails, enriched in E/D/S with frequent G/P, including canonical chaperone EEVD/H/KDEL‑proximal tails and analogous acidic linkers/termini used for partner docking
Activation pattern: Regional activation over long, low‑complexity blo... | Acidic low‑complexity intrinsically disordered segments, most often C‑terminal tails, enriched in E/D/S with frequent G/P, including canonical chaperone EEVD/H/KDEL‑proximal tails and analogous acidic linkers/termini used for partner docking | Regional activation over long, low‑complexity blocks (tens to >100 residues), with a strong bias toward high residue numbers (frequently the extreme C‑terminus); compositionally biased to E/D/S with G/P; occasional spikes at terminal tetrapeptides (e.g., EEVD, H/KDEL) | Compositional bias | - Hsp70/Hsp110 (BiP/GRP78, HSP70s, HSP110/GRP170): activation on the acidic, disordered C‑terminal tails, including cytosolic EEVD/EEDV endings or ER‑luminal H/KDEL‑proximal acidic regions that recruit TPR‑domain co‑chaperones.
- Actin and actin‑related proteins (ARPs; bacterial/archaeal actin‑like proteins): activatio... | 4,520,735 | 3.828891 | 27.265625 | [
{
"activation": 27.265625,
"uniref_id": "UPI003752EABC"
},
{
"activation": 26.890625,
"uniref_id": "UPI001F4FEF79"
},
{
"activation": 26.796875,
"uniref_id": "A0A3C1RMN6"
},
{
"activation": 26.765625,
"uniref_id": "A0A2W4L847"
},
{
"activation": 26.59375,
"uni... | [
{
"activation": 24.081256866455078,
"uniprot_id": "P38788"
},
{
"activation": 22.85580062866211,
"uniprot_id": "Q8K0U4"
},
{
"activation": 22.11968231201172,
"uniprot_id": "Q60432"
},
{
"activation": 22.024002075195312,
"uniprot_id": "Q0VDF9"
},
{
"activation": 21... | [
3774,
12866,
14889,
14180,
4007,
10720,
12023,
8109,
8917,
16202
] | 0.164274 |
64 | Summary: Short coil/turn motifs at helix boundaries and re-entrant loops, enriched in small/polar and proline residues; prototypically the potassium-channel pore-helix→selectivity-filter hairpin (TVGYG/GYG) and, by extension, analogous inter-helix linkers and proline-rich hairpins in repeat/scaffold proteins
Activation... | Short coil/turn motifs at helix boundaries and re-entrant loops, enriched in small/polar and proline residues; prototypically the potassium-channel pore-helix→selectivity-filter hairpin (TVGYG/GYG) and, by extension, analogous inter-helix linkers and proline-rich hairpins in repeat/scaffold proteins | Residue-level peaks confined to short coils/turns. Strongest at the pore-helix C-terminus and selectivity filter in K+ pores and in the re-entrant loops between paired transmembrane helices; in soluble scaffolds, peaks on the loop/turn connectors between helices (e.g., HEAT repeats); in ER lectins, repeated peaks acros... | Ligand-binding site | - Two-pore domain potassium channels (K2P): activation at pore-helix ends and the selectivity filter (GYG triad/K+-binding carbonyl cage).
- Trk/Ktr potassium uptake transporters: activation on re-entrant loops between TM1-2, 3-4, 5-6, 7-8 that house filter-like motifs.
- HEAT-repeat regulatory subunits (e.g., PP4R1-li... | 60,468,862 | 1.235437 | 26.59375 | [
{
"activation": 26.59375,
"uniref_id": "A0A8J1ZQI8"
},
{
"activation": 26.453125,
"uniref_id": "A0A8J2BEL5"
},
{
"activation": 26.28125,
"uniref_id": "A0A260Z3N2"
},
{
"activation": 26.25,
"uniref_id": "UPI0031DBB434"
},
{
"activation": 26.203125,
"uniref_id":... | [
{
"activation": 25.700923919677734,
"uniprot_id": "Q9V785"
},
{
"activation": 25.3668212890625,
"uniprot_id": "P42171"
},
{
"activation": 25.215496063232422,
"uniprot_id": "Q21194"
},
{
"activation": 25.213462829589844,
"uniprot_id": "O60239"
},
{
"activation": 24... | [
2504,
13757,
9340,
11421,
9524,
1175,
5271,
6183,
11122,
11980
] | 0.168065 |
65 | Summary: Hydrophobic core positions of alpha-helical segments in small-molecule transporters and transporter systems (predominantly multi-pass secondary carriers such as MFS, DMT/EamA, NCS/ENT, but also ABC-type systems), capturing generic helix–bundle/interface architecture rather than family-specific catalytic motifs... | Hydrophobic core positions of alpha-helical segments in small-molecule transporters and transporter systems (predominantly multi-pass secondary carriers such as MFS, DMT/EamA, NCS/ENT, but also ABC-type systems), capturing generic helix–bundle/interface architecture rather than family-specific catalytic motifs. | Residue-level peaks concentrated within many distinct transmembrane alpha-helices across permease subunits; strongest on Leu/Ile/Val/Phe/Tyr/Trp with frequent small residues (Ala/Ser/Thr/Gly), minimal in loops/disordered regions; occasional peaks at helix starts/ends or short adjacent helical segments, including cytoso... | Membrane-associated | unchanged. | 50,255,132 | 1.420453 | 20.734375 | [
{
"activation": 20.734375,
"uniref_id": "X6D1Z0"
},
{
"activation": 20.71875,
"uniref_id": "A0A6N7C6K2"
},
{
"activation": 20.59375,
"uniref_id": "V7F6W3"
},
{
"activation": 20.5625,
"uniref_id": "UPI002FC310C5"
},
{
"activation": 20.5,
"uniref_id": "A0A653R1K... | [
{
"activation": 18.4398250579834,
"uniprot_id": "P28246"
},
{
"activation": 17.894332885742188,
"uniprot_id": "P0AEY8"
},
{
"activation": 17.601999282836914,
"uniprot_id": "P31442"
},
{
"activation": 17.564373016357422,
"uniprot_id": "P0DX21"
},
{
"activation": 17... | [
5544,
2661,
7851,
12024,
13058,
14232,
9351,
13512,
3857,
15031
] | 0.470482 |
66 | Summary: Short consensus segments that form the blades of beta-propeller domains, in both extracellular/periplasmic and cytosolic contexts, including cases where the propeller itself is catalytic (e.g., GH33/GH43, PQQ-dependent dehydrogenases).
Activation pattern: Multiple short regional peaks per protein, typically on... | Short consensus segments that form the blades of beta-propeller domains, in both extracellular/periplasmic and cytosolic contexts, including cases where the propeller itself is catalytic (e.g., GH33/GH43, PQQ-dependent dehydrogenases). | Multiple short regional peaks per protein, typically one or more within each repeat/blade; peaks lie in or adjacent to conserved blade motifs and connecting loops/strand edges. Little to no activation in transmembrane segments, cytosolic tails, or non-propeller catalytic cores; when the propeller is catalytic, activati... | Domain | - Integrin α subunits: activation on FG-GAP repeats assembling the N-terminal propeller; absent from TM/cytoplasmic regions.
- Semaphorin/Plexin pathway receptors: activation across Sema-domain propeller blades mediating extracellular ligand recognition.
- BTB/Kelch E3 adaptor proteins: activation on Kelch repeats form... | 35,342,763 | 1.772472 | 15.28125 | [
{
"activation": 15.28125,
"uniref_id": "UPI0031DC392E"
},
{
"activation": 15.1171875,
"uniref_id": "UPI003B5C483A"
},
{
"activation": 15.1171875,
"uniref_id": "A0A371JUA2"
},
{
"activation": 15.109375,
"uniref_id": "UPI0010A64BBE"
},
{
"activation": 15.0703125,
... | [
{
"activation": 13.608070373535156,
"uniprot_id": "Q5ADT1"
},
{
"activation": 13.50648307800293,
"uniprot_id": "P32634"
},
{
"activation": 13.180601119995117,
"uniprot_id": "Q8L7U5"
},
{
"activation": 13.105443954467773,
"uniprot_id": "Q9Y2U9"
},
{
"activation": 1... | [
7527,
15382,
14884,
6170,
11022,
14765,
4718,
6895,
15379,
15164
] | 0.391348 |
67 | Summary: Hydrophobic loop/turn residues within β-sheet-rich immunoglobulin-like superfamily modules—especially Fibronectin type III, Ig-like C2, PKD, and related bacterial Ig-like/FN3-like repeats—found in repeated domain architectures of adhesion/receptor and scaffolding proteins; prevalent in extracellular proteins b... | Hydrophobic loop/turn residues within β-sheet-rich immunoglobulin-like superfamily modules—especially Fibronectin type III, Ig-like C2, PKD, and related bacterial Ig-like/FN3-like repeats—found in repeated domain architectures of adhesion/receptor and scaffolding proteins; prevalent in extracellular proteins but also p... | Residue-level peaks that recur once per repeat domain; narrowly localized to single L/V/I/A/P residues in short coil/turn segments linking β-strands of Ig/FN3/PKD-like β-sandwich folds; repeated across tandem domains; absent from transmembrane helices but not restricted to extracellular regions. | Structural motif | - IgCAM/axon guidance receptors (e.g., ROBO/SAX-3): single L/V peaks in loops of Ig C2 and FN3 domains across the ectodomain.
- Fibronectin type III repeat proteins: sharp single-residue peaks once per FN3 domain across multiple repeats.
- Receptor tyrosine kinases and phosphatases (e.g., EphB2, PTPRJ): isolated loop h... | 44,916,187 | 1.532767 | 23.765625 | [
{
"activation": 23.765625,
"uniref_id": "A0A1F4ABE5"
},
{
"activation": 23.671875,
"uniref_id": "A0A2V9F8N6"
},
{
"activation": 23.609375,
"uniref_id": "UPI0015D84AF8"
},
{
"activation": 23.421875,
"uniref_id": "A0A427A3C1"
},
{
"activation": 23.328125,
"unire... | [
{
"activation": 22.28870391845703,
"uniprot_id": "Q4ZHG4"
},
{
"activation": 21.775821685791016,
"uniprot_id": "Q6GQT9"
},
{
"activation": 21.517032623291016,
"uniprot_id": "P32018"
},
{
"activation": 21.378820419311523,
"uniprot_id": "Q04164"
},
{
"activation": 2... | [
10175,
1122,
325,
7173,
3841,
8270,
9462,
764,
11674,
10036
] | 0.376215 |
68 | Summary: Alpha-helical transmembrane segments and, especially, the helix–loop junctions at the ends of these helices in multi-pass membrane transporters and channels, often featuring small-residue (glycine/alanine) motifs used for helix packing and gating
Activation pattern: Regional activation across long hydrophobic ... | Alpha-helical transmembrane segments and, especially, the helix–loop junctions at the ends of these helices in multi-pass membrane transporters and channels, often featuring small-residue (glycine/alanine) motifs used for helix packing and gating | Regional activation across long hydrophobic stretches with multiple peaks per protein; strongest at transmembrane helix termini and short connecting loops, tapering into the adjacent helix; broad activation across many TM segments within permease domains and little in large soluble regions; occasionally highlights glyc... | Membrane-associated | - TRAP transporter permease (large permease subunit): activation on several TM helices, with a sharp peak at a helix/turn overlap inside the membrane and additional peaks in other helices; little/no signal in the small permease subunit.
- PHO family phosphate permease: repeated peaks at the interfaces where TM helices ... | 13,655,971 | 2.723388 | 30.046875 | [
{
"activation": 30.046875,
"uniref_id": "UPI000C832B1B"
},
{
"activation": 29.9375,
"uniref_id": "A0A921GH28"
},
{
"activation": 29.8125,
"uniref_id": "A0A7C1BV18"
},
{
"activation": 29.453125,
"uniref_id": "A0A8S0FZV8"
},
{
"activation": 29.40625,
"uniref_id"... | [
{
"activation": 27.444372177124023,
"uniprot_id": "P0ABN9"
},
{
"activation": 26.15349578857422,
"uniprot_id": "P0ABP3"
},
{
"activation": 25.51202392578125,
"uniprot_id": "P55069"
},
{
"activation": 24.306718826293945,
"uniprot_id": "A0A0H2VAP9"
},
{
"activation"... | [
10586,
9499,
9639,
15811,
3659,
16129,
3543,
10244,
12955,
12376
] | 0.17837 |
69 | Summary: Periodic aromatic/glycine/histidine/acidic cluster detector with a strong bias toward beta-strand repeat architectures—most notably WD40/radial-spoke beta-propellers and MORN repeats—but also recognizing similar periodically spaced motifs in flexible low-complexity repeats and structured loops of diverse prote... | Periodic aromatic/glycine/histidine/acidic cluster detector with a strong bias toward beta-strand repeat architectures—most notably WD40/radial-spoke beta-propellers and MORN repeats—but also recognizing similar periodically spaced motifs in flexible low-complexity repeats and structured loops of diverse proteins (incl... | Residue-level peaks that recur at regular intervals across repeat arrays; strongest and most regular on beta-strands at repeat starts/ends, but can also occur in low-complexity/disordered G/F/Y-rich segments and in periplasmic/loop regions; minimal outside these arrays. | Repeat | - MORN repeat proteins: strong peaks at the beginning of each repeat beta-strand.
- Radial spoke head WD-repeat proteins: activation at GH–W/Y–D-like positions across beta-propeller blades.
- Outer membrane porins (VDAC/TOM40): periodic but sometimes weaker activation on beta-barrel strands.
- Secreted carbohydrate-act... | 7,155,735 | 3.369651 | 23.046875 | [
{
"activation": 23.046875,
"uniref_id": "A0A433SD77"
},
{
"activation": 22.859375,
"uniref_id": "UPI001BCC9A40"
},
{
"activation": 22.765625,
"uniref_id": "A0A512CEV2"
},
{
"activation": 22.734375,
"uniref_id": "UPI00189AA1CE"
},
{
"activation": 22.609375,
"un... | [
{
"activation": 13.287347793579102,
"uniprot_id": "Q8WTS6"
},
{
"activation": 11.295007705688477,
"uniprot_id": "Q8IIT5"
},
{
"activation": 11.08403491973877,
"uniprot_id": "Q6VTH5"
},
{
"activation": 11.070294380187988,
"uniprot_id": "Q8VIG3"
},
{
"activation": 1... | [
962,
14715,
10786,
10751,
16039,
7630,
14805,
5534,
5238,
13696
] | 0.238704 |
70 | Summary: Cell-envelope/extracellular anchoring and linker segments: the feature highlights low‑complexity, Ser/Thr/Pro/Gly‑rich, disordered regions in secreted or periplasmic proteins that mediate surface display/assembly and often interface with cell-wall glycans (including GPI‑anchor ω‑site regions and periplasmic co... | Cell-envelope/extracellular anchoring and linker segments: the feature highlights low‑complexity, Ser/Thr/Pro/Gly‑rich, disordered regions in secreted or periplasmic proteins that mediate surface display/assembly and often interface with cell-wall glycans (including GPI‑anchor ω‑site regions and periplasmic coiled‑coil... | Bimodal: (1) long regional activation across flexible, low‑complexity segments flanking signal peptides or at C‑terminal pre‑anchor regions (often enriched in Ser/Thr/Pro/Gly and charged residues); (2) sharp residue‑level peaks at single conserved positions in enzymes (e.g., catalytic Cys or glycine‑adjacent active‑sit... | Disorder | - Bacteriophage spanins (inner-membrane subunits): strong regional activation over the periplasmic coiled‑coil and adjacent disordered loop, including the interchain disulfide‑forming cysteine.
- Plant non-specific lipid transfer proteins (GPI-anchored): activation on the C‑terminal disordered GPI‑anchor signal/ω‑site–... | 7,568,277 | 3.3136 | 13.90625 | [
{
"activation": 13.90625,
"uniref_id": "UPI0028AC2457"
},
{
"activation": 13.8984375,
"uniref_id": "A0A7X0VDT1"
},
{
"activation": 13.890625,
"uniref_id": "S2XLG9"
},
{
"activation": 13.8828125,
"uniref_id": "A0AAE4L4M0"
},
{
"activation": 13.875,
"uniref_id":... | [
{
"activation": 13.202457427978516,
"uniprot_id": "Q38580"
},
{
"activation": 9.858179092407227,
"uniprot_id": "A4ZFB2"
},
{
"activation": 7.844074249267578,
"uniprot_id": "Q9XJJ6"
},
{
"activation": 6.457607269287109,
"uniprot_id": "P39504"
},
{
"activation": 5.1... | [
12604,
12890,
35,
7747,
16264,
6282,
3020,
4997,
3953,
8222
] | 0.26432 |
71 | Summary: Leucine-rich repeat (LRR) architecture, with a strong preference for the convex-face C‑terminal half of each LRR unit (the helix/helix–turn segment) and adjacent flexible linkers; the feature fires periodically across tandem LRR arrays and also highlights LRR‑like leucine-rich helices even when repeats are una... | Leucine-rich repeat (LRR) architecture, with a strong preference for the convex-face C‑terminal half of each LRR unit (the helix/helix–turn segment) and adjacent flexible linkers; the feature fires periodically across tandem LRR arrays and also highlights LRR‑like leucine-rich helices even when repeats are unannotated | - Domain-level: Broad, continuous activation across LRR domains; minimal/absent in non-LRR modules (e.g., signal peptides, transmembrane segments, TIR, NACHT, NB‑ARC, disordered tails).
- Residue-level: Repetitive 5–12 residue peaks per repeat, concentrated in the C‑terminal portion of each LRR (the convex-face alpha-... | Domain | - Toll-like receptors (LRR ectodomains): periodic peaks across extracellular LRRs, localized to the C‑terminal half/helix of each repeat; no activation in signal peptide, transmembrane, or TIR domains.
- NOD-like receptors (e.g., NLRC5): silent over the NACHT; strong, repeat-by-repeat peaks throughout the C‑terminal LR... | 10,418,849 | 2.993949 | 22.078125 | [
{
"activation": 22.078125,
"uniref_id": "A0A915CVJ7"
},
{
"activation": 22,
"uniref_id": "A0AAD5KI91"
},
{
"activation": 21.96875,
"uniref_id": "A0A914RHH8"
},
{
"activation": 21.96875,
"uniref_id": "A0A8H7S9S4"
},
{
"activation": 21.953125,
"uniref_id": "A0AA... | [
{
"activation": 21.097919464111328,
"uniprot_id": "Q7Z6M2"
},
{
"activation": 20.813993453979492,
"uniprot_id": "Q8BMI0"
},
{
"activation": 20.072099685668945,
"uniprot_id": "Q96ME1"
},
{
"activation": 20.057891845703125,
"uniprot_id": "Q5VMP0"
},
{
"activation": ... | [
5430,
1144,
6363,
6513,
3850,
8699,
5566,
16267,
1610,
15074
] | 0.460822 |
72 | Summary: N-terminal secretory-pathway/extracellular module detecting exported/lumenal segments and adjacent signal‑anchor/first transmembrane helix, with a strong bias toward disulfide‑rich, membrane‑active secreted proteins (notably sPLA2 and related toxins) and some viral capsid regions; secondary mode: recognizes N‑... | N-terminal secretory-pathway/extracellular module detecting exported/lumenal segments and adjacent signal‑anchor/first transmembrane helix, with a strong bias toward disulfide‑rich, membrane‑active secreted proteins (notably sPLA2 and related toxins) and some viral capsid regions; secondary mode: recognizes N‑terminal ... | Long, contiguous N‑terminal regional activation spanning signal peptide or signal‑anchor/first TM into proximal lumenal/extracellular domain; for soluble secreted proteins, covers cysteine/glycine‑rich domains; minimal on cytosolic tails; occasional minor peaks on additional TMs; not centered on catalytic residues. Als... | Post-translational modification | 10,877,933 | 2.950829 | 16.34375 | [
{
"activation": 16.34375,
"uniref_id": "A0A1T0CSH9"
},
{
"activation": 16.140625,
"uniref_id": "A0A7T5BIS1"
},
{
"activation": 16.140625,
"uniref_id": "UPI00135C8311"
},
{
"activation": 16.125,
"uniref_id": "UPI002D6FCE22"
},
{
"activation": 16.125,
"uniref_id... | [
{
"activation": 14.75123405456543,
"uniprot_id": "A4NBN9"
},
{
"activation": 10.38398551940918,
"uniprot_id": "P0CV89"
},
{
"activation": 10.120767593383789,
"uniprot_id": "Q9XG80"
},
{
"activation": 10.050639152526855,
"uniprot_id": "Q8GV50"
},
{
"activation": 9.... | [
12615,
9328,
13919,
13257,
2808,
14124,
1080,
8639,
13877,
6411
] | 0.259658 | |
73 | Summary: Pepsin/retropepsin-fold aspartyl endopeptidases (EC 3.4.23.-), including their zymogen activation peptides; the feature recognizes the pepsin-like catalytic domain and, in precursors, the N-terminal prosegment that occludes the active site. Rarely, it also triggers on long D/G-rich low-complexity repeats (e.g.... | Pepsin/retropepsin-fold aspartyl endopeptidases (EC 3.4.23.-), including their zymogen activation peptides; the feature recognizes the pepsin-like catalytic domain and, in precursors, the N-terminal prosegment that occludes the active site. Rarely, it also triggers on long D/G-rich low-complexity repeats (e.g., mycobac... | Domain-level/regional activation: long, contiguous activation across the folded pepsin-like protease domain; in zymogens, strong activation on the N-terminal activation (pro)peptide with an abrupt drop at the propeptide–mature junction; occasional local minima at the catalytic Asp despite enrichment for D/G around cata... | Domain | - Secreted gastric aspartyl proteases (pepsin/gastricsin): high activation on the propeptide and sharp loss at the mature N-terminus
- Cathepsin D–like lysosomal/vacuolar proteases and fungal pepsin-like/yapsin enzymes: broad activation over the catalytic domain (sometimes extending into the prosegment)
- Plant A1 aspa... | 4,044,546 | 3.940196 | 19.015625 | [
{
"activation": 19.015625,
"uniref_id": "A0AAD5DIU6"
},
{
"activation": 19,
"uniref_id": "A0A7S2TYP3"
},
{
"activation": 18.734375,
"uniref_id": "A0A954YIM1"
},
{
"activation": 18.703125,
"uniref_id": "A0A1G2YGQ8"
},
{
"activation": 18.703125,
"uniref_id": "A0... | [
{
"activation": 14.39220142364502,
"uniprot_id": "A8Q7G1"
},
{
"activation": 14.273688316345215,
"uniprot_id": "Q6PTV2"
},
{
"activation": 14.261211395263672,
"uniprot_id": "Q9XFX3"
},
{
"activation": 14.106738090515137,
"uniprot_id": "P56817"
},
{
"activation": 1... | [
3423,
1344,
14851,
9961,
11865,
2071,
2978,
6419,
7882,
6177
] | 0.254871 |
74 | Summary: Radical SAM [4Fe–4S] cluster–binding motif and immediate context: recognition of the canonical CxxxCxxC cysteine triad (often preceded by glycine and flanked by basic residues) within the Radical SAM core of cobalamin-dependent C-methyltransferases and tRNA methylthiotransferase/methylthiolating enzymes, with ... | Radical SAM [4Fe–4S] cluster–binding motif and immediate context: recognition of the canonical CxxxCxxC cysteine triad (often preceded by glycine and flanked by basic residues) within the Radical SAM core of cobalamin-dependent C-methyltransferases and tRNA methylthiotransferase/methylthiolating enzymes, with broader a... | Primarily short, residue-level peaks within an ~8–20 aa window centered on the CxxxCxxC motif (typically mid-protein); in some cases broadened to regional/domain-level activation across the Radical SAM core, with strongest signal on residues flanking the three cysteines and occasionally reduced signal on the cysteines ... | Ligand-binding site | - Cobalamin-dependent Radical SAM C-methylases (e.g., hopanoid C-2 methylase): strong regional activation across the Radical SAM core, peaking around the Fe–S CxxxCxxC motif while the cysteines themselves may be less highlighted
- tRNA methylthiotransferases (MiaB/MtaB family): sharp, short-window activation centered o... | 3,497,410 | 4.085543 | 20.890625 | [
{
"activation": 20.890625,
"uniref_id": "A0A7C0X4A8"
},
{
"activation": 20.890625,
"uniref_id": "A0A933GET0"
},
{
"activation": 20.703125,
"uniref_id": "A0A8T6N2Y8"
},
{
"activation": 20.640625,
"uniref_id": "UPI0034A17867"
},
{
"activation": 20.625,
"uniref_i... | [
{
"activation": 19.433826446533203,
"uniprot_id": "Q55373"
},
{
"activation": 19.368070602416992,
"uniprot_id": "P26168"
},
{
"activation": 19.118282318115234,
"uniprot_id": "Q60AV6"
},
{
"activation": 18.88002586364746,
"uniprot_id": "B3QHD1"
},
{
"activation": 1... | [
1933,
5370,
3853,
4215,
6325,
1936,
2389,
7917,
814,
2
] | 0.169853 |
75 | Summary: Glycine-rich, flexible loop motifs (GG/GS/GT-enriched and small/polar) that flank binding/interaction pockets and hinges, often immediately adjacent to an aromatic residue (Trp/Tyr) used for stacking or capping. These loops frequently serve as phosphate/nucleotide- or carbohydrate/pterin-binding loops or inter... | Glycine-rich, flexible loop motifs (GG/GS/GT-enriched and small/polar) that flank binding/interaction pockets and hinges, often immediately adjacent to an aromatic residue (Trp/Tyr) used for stacking or capping. These loops frequently serve as phosphate/nucleotide- or carbohydrate/pterin-binding loops or interfacial tu... | Predominantly residue-level peaks on short glycine-rich loop residues and neighboring aromatics; often extends to regional activation across longer Gly/Ser/Thr–rich stretches (e.g., repeat β-solenoids or low-complexity linkers); occasionally covers an entire flexible loop/hinge segment within a domain. | Ligand-binding site | - Phage tail fiber/spike and receptor-binding proteins: activation on gly/ser-rich receptor-binding loops in β-solenoid/β-helix repeats, frequently including GG–X–W/Y motifs where aromatics stack against sugars.
- Soluble inorganic pyrophosphatases: activation on glycine-/small-residue phosphate-binding loops at the me... | 4,939,448 | 3.740312 | 14.953125 | [
{
"activation": 14.953125,
"uniref_id": "A0A8S5NI46"
},
{
"activation": 14.8515625,
"uniref_id": "UPI000E4F32DC"
},
{
"activation": 14.8125,
"uniref_id": "UPI002587F327"
},
{
"activation": 14.796875,
"uniref_id": "UPI002597EB96"
},
{
"activation": 14.78125,
"u... | [
{
"activation": 13.258899688720703,
"uniprot_id": "P13390"
},
{
"activation": 12.479647636413574,
"uniprot_id": "P0DJY6"
},
{
"activation": 12.444561004638672,
"uniprot_id": "P76192"
},
{
"activation": 11.922065734863281,
"uniprot_id": "Q51739"
},
{
"activation": ... | [
3602,
8993,
3115,
12200,
7681,
15924,
13162,
1943,
14993,
5563
] | 0.26189 |
76 | Summary: Short glycine/threonine‑rich catalytic loop with a Thr–x–Gly–x–Asp (T–x–G–x–D) core that binds/positions the phosphate and Mg2+ and places a catalytic Asp for phosphoryl transfer in small‑molecule kinases (ribokinase/GHMP‑like); the same sequence/structural signature is weakly recognized in analogous glycine/t... | Short glycine/threonine‑rich catalytic loop with a Thr–x–Gly–x–Asp (T–x–G–x–D) core that binds/positions the phosphate and Mg2+ and places a catalytic Asp for phosphoryl transfer in small‑molecule kinases (ribokinase/GHMP‑like); the same sequence/structural signature is weakly recognized in analogous glycine/threonine ... | Residue‑level peaks confined to short contiguous windows (~18–25 aa), usually in the C‑terminal half (~residues 200–270) corresponding to the mobile/lid loop at the mouth of the active site; several ThiD/HMP kinases show an additional weaker N‑terminal glycine‑rich patch. The activated segment is enriched in small/pola... | Catalytic function | - Thiamine‑biosynthesis kinases (ThiD/HMP kinase; ThiM/thiazole kinase): activation on the T–x–G–x–D loop that closes over ATP and coordinates Mg2+; some show a secondary N‑terminal glycine‑rich ATP‑binding patch.
- Ribokinase‑like sugar/nucleoside kinases (ribokinase, adenosine kinase, xylulokinase, ribulokinase): act... | 21,298,523 | 2.278928 | 18.671875 | [
{
"activation": 18.671875,
"uniref_id": "UPI0018EC6BE6"
},
{
"activation": 18.5625,
"uniref_id": "UPI0031EEDEBA"
},
{
"activation": 18.515625,
"uniref_id": "UPI0004939A29"
},
{
"activation": 18.46875,
"uniref_id": "UPI0035265751"
},
{
"activation": 18.359375,
... | [
{
"activation": 16.688600540161133,
"uniprot_id": "Q8Z6Q3"
},
{
"activation": 16.221332550048828,
"uniprot_id": "Q49396"
},
{
"activation": 16.194704055786133,
"uniprot_id": "O00764"
},
{
"activation": 16.14936065673828,
"uniprot_id": "Q9WZP7"
},
{
"activation": 1... | [
15337,
7291,
254,
7078,
6361,
9437,
13292,
9692,
4266,
8552
] | 0.340607 |
77 | Summary: Heptad‑repeat alpha‑helical rod positions in long coiled‑coil/spectrin‑like domains—i.e., a fixed register within (abcdefg)n repeats of extended coiled‑coils and related helical rods used by eukaryotic scaffolds (cilia/centrosome components, Golgi tethers, cytolinkers), largely independent of side‑chain identi... | Heptad‑repeat alpha‑helical rod positions in long coiled‑coil/spectrin‑like domains—i.e., a fixed register within (abcdefg)n repeats of extended coiled‑coils and related helical rods used by eukaryotic scaffolds (cilia/centrosome components, Golgi tethers, cytolinkers), largely independent of side‑chain identity and of... | Regional activation across long alpha‑helical rods with sharp residue‑level peaks repeating every ~7 residues (heptad periodicity); strong in continuous coiled‑coil/spectrin regions; minimal in disordered termini and compact globular domains (e.g., WW, zinc fingers) | Structural motif | - Coiled‑coil scaffold (CCDC family, e.g., CCDC146): periodic peaks throughout the annotated coiled‑coil rod; no activation in disordered N‑terminus.
- Axonemal outer dynein arm coiled‑coil subunit: activation confined to the long central coiled‑coil; absent from disordered ends.
- Centriolar scaffold (Centlein): dense... | 18,111,978 | 2.440992 | 17.109375 | [
{
"activation": 17.109375,
"uniref_id": "A0A8S4B1X2"
},
{
"activation": 16.9375,
"uniref_id": "A0A8D0A2K9"
},
{
"activation": 16.796875,
"uniref_id": "A0A9Q0EB62"
},
{
"activation": 16.765625,
"uniref_id": "A0A7N8Y0L6"
},
{
"activation": 16.671875,
"uniref_id"... | [
{
"activation": 15.573102951049805,
"uniprot_id": "Q66H60"
},
{
"activation": 15.146459579467773,
"uniprot_id": "E9Q7R9"
},
{
"activation": 14.801487922668457,
"uniprot_id": "Q2M329"
},
{
"activation": 14.797490119934082,
"uniprot_id": "Q6ZMZ3"
},
{
"activation": ... | [
7006,
264,
4575,
4662,
11021,
1925,
12436,
2647,
5280,
13392
] | 0.407724 |
78 | Summary: A short, basic/aromatic helix-end/turn motif that makes direct DNA contacts: the loop/helix-terminus element of helix–turn–helix–like folds (Myb/SANT/ARID/DnaA) and the analogous DNA‑proximal loop carrying the catalytic Tyr in tyrosine recombinases. It captures the major‑groove recognition/turn segment enriche... | A short, basic/aromatic helix-end/turn motif that makes direct DNA contacts: the loop/helix-terminus element of helix–turn–helix–like folds (Myb/SANT/ARID/DnaA) and the analogous DNA‑proximal loop carrying the catalytic Tyr in tyrosine recombinases. It captures the major‑groove recognition/turn segment enriched in Lys/... | Residue-level peaks within very short (≈4–8 aa) stretches; commonly two discrete hotspots per protein. Peaks sit at C-termini of α-helices transitioning into loops in HTH-like domains, or at the catalytic Tyr-containing loop of tyrosine recombinases. Composition is Lys/Arg-rich with Gly/Leu and occasional Tyr/Trp. | Ligand-binding site | - Tyrosine recombinases/integrases/resolvases: activation on the catalytic loop around the conserved Tyr with nearby His/Arg (often including a Trp), and/or the N‑terminal HTH DNA-binding segment used for site recognition.
- Plant and other eukaryotic Myb transcription factors (R2R3‑MYB and single‑repeat Myb): two shor... | 27,053,044 | 2.039766 | 21.5 | [
{
"activation": 21.5,
"uniref_id": "UPI002635EA21"
},
{
"activation": 21.21875,
"uniref_id": "UPI0024A991DD"
},
{
"activation": 21.109375,
"uniref_id": "UPI0033A8ABCF"
},
{
"activation": 21.078125,
"uniref_id": "A0A960R6T1"
},
{
"activation": 21.03125,
"uniref... | [
{
"activation": 18.25108528137207,
"uniprot_id": "P20214"
},
{
"activation": 18.063690185546875,
"uniprot_id": "P03870"
},
{
"activation": 17.86935806274414,
"uniprot_id": "O34336"
},
{
"activation": 17.576297760009766,
"uniprot_id": "P0CE19"
},
{
"activation": 17... | [
7030,
7606,
16270,
15848,
181,
10343,
9136,
3347,
4261,
3699
] | 0.312408 |
79 | Summary: Conserved phosphate‑interacting/catalytic interface: a subdomain or motif that binds or processes phosphate groups (from nucleic acids, nucleotides, or phosphoinositides), enriched in metal‑chelating acidic residues and Lys/Arg/Gly/Ser‑rich loops; typically corresponds to an N‑terminal or pre‑catalytic region ... | Conserved phosphate‑interacting/catalytic interface: a subdomain or motif that binds or processes phosphate groups (from nucleic acids, nucleotides, or phosphoinositides), enriched in metal‑chelating acidic residues and Lys/Arg/Gly/Ser‑rich loops; typically corresponds to an N‑terminal or pre‑catalytic region of nuclea... | Predominantly regional activation over a single structured subdomain (often 30–200 aa), with superimposed sharp residue‑level peaks at key acidic/Ser/Lys/Arg or metal‑binding positions; in some secreted proteases and zymogens the feature contracts to one or a few hallmark residues; activated segments are usually well‑s... | Ligand-binding site | - CRISPR Cascade large subunit (Cas8/Cse1): strong domain‑level activation confined to the N‑terminal DNA‑contacting domain (helices/strands/loops).
- Fanconi‑associated nuclease FAN1: a single pre‑VRR‑NUC segment with a peak at a Mn2+‑binding glutamate, marking the phosphate‑processing interface adjacent to the nuclea... | 4,704,203 | 3.789109 | 12.804688 | [
{
"activation": 12.8046875,
"uniref_id": "UPI00307BEC6F"
},
{
"activation": 12.7734375,
"uniref_id": "A0A496R269"
},
{
"activation": 12.765625,
"uniref_id": "A0AAW5K156"
},
{
"activation": 12.75,
"uniref_id": "UPI00307895A2"
},
{
"activation": 12.7421875,
"uni... | [
{
"activation": 9.093219757080078,
"uniprot_id": "Q9LSS3"
},
{
"activation": 9.072354316711426,
"uniprot_id": "Q8NCE0"
},
{
"activation": 8.993608474731445,
"uniprot_id": "Q02825"
},
{
"activation": 8.745386123657227,
"uniprot_id": "Q8TFH7"
},
{
"activation": 8.69... | [
9450,
10246,
4218,
9756,
7769,
15652,
13981,
60,
11744,
4603
] | 0.389358 |
80 | Summary: Low-complexity, intrinsically disordered regulatory segments in nuclear gene-expression regulators—long S/T/P/G/Q/Y-rich tracts (often with PxxP and/or glycine-rich/RGG motifs) that serve as transactivation and interaction regions flanking, and excluding, the structured DNA- or RNA-binding domains
Activation p... | Low-complexity, intrinsically disordered regulatory segments in nuclear gene-expression regulators—long S/T/P/G/Q/Y-rich tracts (often with PxxP and/or glycine-rich/RGG motifs) that serve as transactivation and interaction regions flanking, and excluding, the structured DNA- or RNA-binding domains | Extended regional activation over long, contiguous IDRs (often N- or C‑terminal or central segments); little to no activation on folded domains such as homeodomains, zinc fingers, forkhead/HMG boxes, RRMs, KH, or YTH domains | Disorder | - GATA transcription factors: strong activation across central disordered low‑complexity regions; no activation on the C‑terminal zinc‑finger DNA‑binding domains
- Homeobox/HOX and paired (PAX) transcription factors: activation on proline/glutamine/serine‑rich transactivation domains flanking the homeodomain or paired ... | 6,961,096 | 3.397228 | 20.1875 | [
{
"activation": 20.1875,
"uniref_id": "A0A814AX88"
},
{
"activation": 20.15625,
"uniref_id": "A0A813X9Z8"
},
{
"activation": 20.046875,
"uniref_id": "A0A819MRI8"
},
{
"activation": 19.890625,
"uniref_id": "A0A815BEX9"
},
{
"activation": 19.859375,
"uniref_id":... | [
{
"activation": 18.934188842773438,
"uniprot_id": "P09082"
},
{
"activation": 18.60736846923828,
"uniprot_id": "P22810"
},
{
"activation": 17.972246170043945,
"uniprot_id": "A7Y7W3"
},
{
"activation": 17.62734603881836,
"uniprot_id": "Q26417"
},
{
"activation": 17... | [
1234,
5236,
7143,
6481,
15922,
4788,
5558,
5803,
14296,
2395
] | 0.446086 |
81 | Summary: Glycine-rich, positively charged N-terminal anion/phosphate-binding segment (often Gly–Gly or Gly–x–Gly motifs) that engages phosphate-bearing ligands (ATP/ADP/PPi, NAD(P), FMN/FAD, CoA, sugar phosphates) or nucleic-acid backbones; captured as a 60–100 aa block most often at the N-terminus and present across m... | Glycine-rich, positively charged N-terminal anion/phosphate-binding segment (often Gly–Gly or Gly–x–Gly motifs) that engages phosphate-bearing ligands (ATP/ADP/PPi, NAD(P), FMN/FAD, CoA, sugar phosphates) or nucleic-acid backbones; captured as a 60–100 aa block most often at the N-terminus and present across multiple f... | Regional activation of a contiguous 60–100 aa basic, glycine-rich stretch near the N-terminus; appears internally when this segment is embedded within larger, multi-domain proteins. | Ligand-binding site | - tRNA dihydrouridine synthases (DusA/B/C): activation on the N-terminal glycine-rich segment that binds FMN phosphate and interfaces with NAD(P)H electron transfer.
- Flavin- and NAD(P)-dependent oxidoreductases (e.g., dihydropyrimidine dehydrogenase, dihydroorotate dehydrogenase, OPDA/Old Yellow Enzyme reductases): a... | 15,403,861 | 2.602947 | 23.78125 | [
{
"activation": 23.78125,
"uniref_id": "A0A813KT47"
},
{
"activation": 23.78125,
"uniref_id": "A0A8D2DIS6"
},
{
"activation": 23.640625,
"uniref_id": "A0A0D6LD86"
},
{
"activation": 23.609375,
"uniref_id": "A0A183KY47"
},
{
"activation": 23.578125,
"uniref_id"... | [
{
"activation": 22.60463523864746,
"uniprot_id": "P96192"
},
{
"activation": 22.131406784057617,
"uniprot_id": "P33371"
},
{
"activation": 21.96012306213379,
"uniprot_id": "P53759"
},
{
"activation": 21.836597442626953,
"uniprot_id": "Q8K582"
},
{
"activation": 21... | [
15976,
8382,
9102,
14087,
14307,
3913,
14129,
10903,
14868,
6739
] | 0.277376 |
82 | Summary: Conserved catalytic core of nucleic‑acid polymerases—most strongly the right‑hand polymerase domain (palm/fingers) of A- and B‑family DNA polymerases, single‑subunit RNA polymerases (T7/RpoT lineage), and viral RNA‑dependent RNA polymerases—with secondary activation on structurally related, nucleic‑acid–proces... | Conserved catalytic core of nucleic‑acid polymerases—most strongly the right‑hand polymerase domain (palm/fingers) of A- and B‑family DNA polymerases, single‑subunit RNA polymerases (T7/RpoT lineage), and viral RNA‑dependent RNA polymerases—with secondary activation on structurally related, nucleic‑acid–processing enzy... | Domain-level, long contiguous stretches within the main enzymatic core; typically mid‑to‑C‑terminal in DNA polymerases and within the initiation/palm segment of single‑subunit RNAPs/RdRPs. Activation is broad around (but not limited to) conserved acidic motifs (e.g., DxD/GDD) and glycine-rich elements, highlighting the... | Domain | - B-family DNA polymerases (eukaryotic Pol delta, archaeal PolB): wide activation across the PolB catalytic domain immediately downstream of essential acidic residues, covering helices/strands that form the polymerase core.
- A-family DNA polymerases (Pol gamma, Pol I/phage-like): strong activation spanning the Klenow-... | 5,854,154 | 3.570414 | 26.203125 | [
{
"activation": 26.203125,
"uniref_id": "A0A9N9TC76"
},
{
"activation": 26.15625,
"uniref_id": "U4UQB4"
},
{
"activation": 26.109375,
"uniref_id": "A0A2D4MW25"
},
{
"activation": 26.046875,
"uniref_id": "A0A7J8CKG2"
},
{
"activation": 25.96875,
"uniref_id": "A... | [
{
"activation": 25.599246978759766,
"uniprot_id": "Q92076"
},
{
"activation": 25.28999137878418,
"uniprot_id": "P54098"
},
{
"activation": 24.70700454711914,
"uniprot_id": "P15801"
},
{
"activation": 24.473621368408203,
"uniprot_id": "Q9Y767"
},
{
"activation": 23... | [
0,
15309,
334,
7546,
1546,
14504,
15772,
15444,
13061,
6060
] | 0.190304 |
83 | Summary: Short aromatic/hydrophobic contact patches that present stacked aromatics for nucleic-acid/ligand binding—typically on beta-strands (e.g., RRM RNP1/RNP2) but also on alpha-helical repeat surfaces (e.g., PUF/Pumilio), and more generally as exposed patches aiding RNA/ligand contact or beta-sheet stabilization ac... | Short aromatic/hydrophobic contact patches that present stacked aromatics for nucleic-acid/ligand binding—typically on beta-strands (e.g., RRM RNP1/RNP2) but also on alpha-helical repeat surfaces (e.g., PUF/Pumilio), and more generally as exposed patches aiding RNA/ligand contact or beta-sheet stabilization across dive... | Residue-level peaks clustering into short motif-like runs on exposed secondary-structure elements—commonly beta-strands, but also short helical turns/repeats; frequently two hotspots per RRM (RNP2 and RNP1) with variable copy number per protein; in uL23, two recurring N-terminal clusters (~positions 20–30 and 60–80); e... | Ligand-binding site | - RRM-containing splicing factors (U1/U2 snRNP proteins, hnRNP/SR proteins): activation on the RNP2 and RNP1 beta-strands that present stacked aromatics for RNA binding
- La-related proteins (LARP family): activation on RRM/La-domain beta-strands at the RNA-binding surface
- Poly(A)/poly(U)-binding proteins: activation... | 98,109,102 | 0.751485 | 14.632813 | [
{
"activation": 14.6328125,
"uniref_id": "A0A662IPX0"
},
{
"activation": 14.6171875,
"uniref_id": "A0A381W1Y1"
},
{
"activation": 14.5703125,
"uniref_id": "UPI001D15ED3A"
},
{
"activation": 14.5625,
"uniref_id": "UPI002073F7C1"
},
{
"activation": 14.4921875,
"... | [
{
"activation": 13.681260108947754,
"uniprot_id": "Q4J989"
},
{
"activation": 13.35593032836914,
"uniprot_id": "P12732"
},
{
"activation": 13.117786407470703,
"uniprot_id": "P71888"
},
{
"activation": 12.798715591430664,
"uniprot_id": "Q9LWB5"
},
{
"activation": 1... | [
7567,
1059,
5203,
9152,
14224,
7525,
960,
7699,
10656,
14543
] | 0.232541 |
84 | Summary: Residue-level markers of secondary-structure register and packing in elongated scaffolds: the feature selects apolar positions within regular elements (especially α-helices in coiled‑coils or transmembrane segments and a conserved β‑strand segment in MHC class I α1) and, at boundaries, glycine hinges that set/... | Residue-level markers of secondary-structure register and packing in elongated scaffolds: the feature selects apolar positions within regular elements (especially α-helices in coiled‑coils or transmembrane segments and a conserved β‑strand segment in MHC class I α1) and, at boundaries, glycine hinges that set/interrupt... | Predominantly sparse, residue-level peaks at specific positions in helices or β‑strands; periodic single‑residue spikes across long, repetitive helical regions (coiled‑coils/adhesin stalks); occasional short 2–5 residue patches at β→α junctions; rare single‑residue peaks at glycine linkers immediately preceding coiled‑... | Structural motif | - Trimeric autotransporter adhesins (SadA-like): repeated activation on leucines within short α‑helical segments of the passenger/stalk (consistent with heptad a/d positions of coiled‑coils).
- Structural maintenance of chromosomes (SMC) coiled‑coil proteins: single glycine peak in a short coil linker immediately befor... | 3,777,752 | 4.008436 | 22.375 | [
{
"activation": 22.375,
"uniref_id": "A0A2H5WYS4"
},
{
"activation": 22.203125,
"uniref_id": "UPI0035C055B7"
},
{
"activation": 22.15625,
"uniref_id": "A0A7C5YMC2"
},
{
"activation": 22.15625,
"uniref_id": "A0A3M2AR93"
},
{
"activation": 22.125,
"uniref_id": "... | [
{
"activation": 19.009124755859375,
"uniprot_id": "E0SIS2"
},
{
"activation": 18.69892120361328,
"uniprot_id": "P0DUH5"
},
{
"activation": 18.59309959411621,
"uniprot_id": "Q9I0F4"
},
{
"activation": 18.210224151611328,
"uniprot_id": "E0SGL7"
},
{
"activation": 17... | [
8815,
13275,
3624,
3387,
7057,
3324,
1074,
8643,
8614,
1489
] | 0.153338 |
85 | Summary: A hydrophobic alpha-helix/topogenic segment detector that preferentially activates on transmembrane helices and N-terminal targeting peptides (signal peptides and organellar transit peptides), with secondary activation on extended helical co-chaperone regions and occasional low-complexity Ser/Thr/Pro- or Cys-r... | A hydrophobic alpha-helix/topogenic segment detector that preferentially activates on transmembrane helices and N-terminal targeting peptides (signal peptides and organellar transit peptides), with secondary activation on extended helical co-chaperone regions and occasional low-complexity Ser/Thr/Pro- or Cys-rich stret... | Regional activation over long, continuous helices (TMs and signal/transit peptides); weaker, shorter activation on incidental hydrophobic/helical or low-complexity segments in soluble proteins. | Membrane-associated | - TRAP transporter small permease subunits (DctQ/SiaQ/YiaM): activation across their single-pass transmembrane helix.
- Two-component sensor histidine kinases: activation over the N-terminal multi-pass sensor transmembranes.
- Twin-arginine translocase subunits (TatB/TatA, including chloroplastic cpTatB): activation on... | 36,802,920 | 1.731988 | 17.125 | [
{
"activation": 17.125,
"uniref_id": "UPI00226C70AC"
},
{
"activation": 16.84375,
"uniref_id": "A0A241VUJ6"
},
{
"activation": 16.765625,
"uniref_id": "UPI0032187653"
},
{
"activation": 16.734375,
"uniref_id": "UPI003463358A"
},
{
"activation": 16.71875,
"unir... | [
{
"activation": 14.031119346618652,
"uniprot_id": "P69425"
},
{
"activation": 12.880227088928223,
"uniprot_id": "Q53705"
},
{
"activation": 12.813611030578613,
"uniprot_id": "O31516"
},
{
"activation": 12.465211868286133,
"uniprot_id": "Q2G1E0"
},
{
"activation": ... | [
13005,
7027,
4100,
12821,
14859,
3535,
6908,
8316,
16119,
10041
] | 0.216253 |
86 | Summary: Eukaryotic multi-pass alpha‑helical transmembrane domains, with a strong preference for the helical bundles of 7‑TM GPCR‑like receptors and MFS/permease transporters, capturing hydrophobic helix cores and helix‑kink/packing motifs; especially enriched in fungal plasma‑membrane signaling and lipid‑metabolic pro... | Eukaryotic multi-pass alpha‑helical transmembrane domains, with a strong preference for the helical bundles of 7‑TM GPCR‑like receptors and MFS/permease transporters, capturing hydrophobic helix cores and helix‑kink/packing motifs; especially enriched in fungal plasma‑membrane signaling and lipid‑metabolic proteins. | Broad, domain‑level activation across multiple predicted transmembrane helices per protein; strongest over the TM5–TM6 pair and intervening intracellular loop (ICL3) in GPCRs; minimal signal in soluble/disordered N‑terminal regions and long C‑terminal tails; residue‑level peaks on hydrophobic/aromatic positions and Gly... | Membrane-associated | - Fungal pheromone GPCRs: activation spans TM1–TM7 with maxima on TM5–TM6 and ICL3; TM1 glycine‑zipper positions highlighted; disordered C‑tail inactive.
- cAMP receptors (7‑TM GPCRs, amoebozoa): continuous activation over TM5–TM6 and the third cytoplasmic loop; little to none in the long disordered tail.
- Metabotropi... | 5,736,664 | 3.590687 | 22.203125 | [
{
"activation": 22.203125,
"uniref_id": "A0A401GHF1"
},
{
"activation": 22.140625,
"uniref_id": "A0A2R6NHP8"
},
{
"activation": 22.09375,
"uniref_id": "A0A409XSB0"
},
{
"activation": 22.09375,
"uniref_id": "A0AAD4BNW3"
},
{
"activation": 22.078125,
"uniref_id"... | [
{
"activation": 17.213315963745117,
"uniprot_id": "A0A0D1E6B3"
},
{
"activation": 14.796296119689941,
"uniprot_id": "D6VTK4"
},
{
"activation": 14.598201751708984,
"uniprot_id": "Q00619"
},
{
"activation": 9.169095039367676,
"uniprot_id": "P31302"
},
{
"activation... | [
2505,
10766,
12779,
3818,
11703,
4319,
14436,
7540,
6987,
872
] | 0.251159 |
87 | Summary: Long, charged alpha-helical coiled-coil/oligomerization rods (often flanked by S/T‑rich low‑complexity segments) that function as scaffold/tether modules for assembling large macromolecular complexes across eukaryotes and viruses (chromosome axis/kinetochore and synaptonemal complex proteins, THO/TREX mRNA‑exp... | Long, charged alpha-helical coiled-coil/oligomerization rods (often flanked by S/T‑rich low‑complexity segments) that function as scaffold/tether modules for assembling large macromolecular complexes across eukaryotes and viruses (chromosome axis/kinetochore and synaptonemal complex proteins, THO/TREX mRNA‑export facto... | Regional/domain-scale activation over contiguous tens to hundreds of residues, strongest on predicted/annotated coiled-coils and continuous alpha-helical rods (often mid‑to‑C‑terminal in long proteins); occasional narrow peaks at short acidic/charged helical or coiled‑coil motifs embedded within otherwise globular enzy... | Structural motif | - Synaptonemal complex and meiotic chromosome-axis proteins (e.g., SYCP2/3-, Red1/Hop1-like): long coiled-coil rods forming the SC/axis.
- Kinetochore/centromere scaffolds (Ndc80/Mis12/CENP complexes): coiled-coil stalks and dimerization helices.
- THO/TREX mRNA-export factors (THOC subunits, UAP56 adaptors): extended ... | 5,542,431 | 3.625132 | 25.46875 | [
{
"activation": 25.46875,
"uniref_id": "A0A176VH81"
},
{
"activation": 25.453125,
"uniref_id": "A0A426WXJ8"
},
{
"activation": 25.046875,
"uniref_id": "A0A8K0MVC7"
},
{
"activation": 25.03125,
"uniref_id": "A0A8K0IJ96"
},
{
"activation": 25,
"uniref_id": "A0A1... | [
{
"activation": 11.917434692382812,
"uniprot_id": "Q9NQ92"
},
{
"activation": 11.190911293029785,
"uniprot_id": "F4ICX9"
},
{
"activation": 9.703420639038086,
"uniprot_id": "P26312"
},
{
"activation": 6.522451400756836,
"uniprot_id": "Q8H0V4"
},
{
"activation": 6.... | [
2565,
3043,
9088,
5039,
8005,
14407,
15303,
14428,
14214,
15564
] | 0.279749 |
88 | Summary: An anion/pyrophosphate–binding glycine-rich loop and adjacent beta–alpha core in soluble alpha–beta enzymes (Rossmann-like and related folds), plus homologous glycine-rich loop segments in certain redox electron-transfer subunits; primarily engages AMP/ADP-bearing cofactors (FAD/FMN/NAD(P), ATP/CoA/AMP) and TP... | An anion/pyrophosphate–binding glycine-rich loop and adjacent beta–alpha core in soluble alpha–beta enzymes (Rossmann-like and related folds), plus homologous glycine-rich loop segments in certain redox electron-transfer subunits; primarily engages AMP/ADP-bearing cofactors (FAD/FMN/NAD(P), ATP/CoA/AMP) and TPP pyropho... | Regional activation over internal domain segments (tens of residues), peaking on glycine-rich anion/pyrophosphate-binding loops (GxGxxG, GxxxxGKT, TPP GDG/NN) and flanking beta1–alpha1–beta2 elements; within mature catalytic domains after targeting peptides. | Ligand-binding site | - Flavoprotein oxidoreductases (FAD/FMN-dependent; acyl‑CoA dehydrogenases, fumarate/succinate/urocanate reductases, complex I/II catalytic subunits): activation on the FAD/FMN-binding Rossmann segment.
- NAD(P)-dependent dehydrogenases (e.g., shikimate dehydrogenase, glycerol/glucose dehydrogenases): activation on the... | 4,969,711 | 3.734204 | 21 | [
{
"activation": 21,
"uniref_id": "UPI00286E1735"
},
{
"activation": 20.9375,
"uniref_id": "A0A8T4FJY0"
},
{
"activation": 20.890625,
"uniref_id": "A0A7G2ILA4"
},
{
"activation": 20.875,
"uniref_id": "A0A2H0P150"
},
{
"activation": 20.859375,
"uniref_id": "A0A5... | [
{
"activation": 20.272960662841797,
"uniprot_id": "P00363"
},
{
"activation": 19.642248153686523,
"uniprot_id": "T2G6Z9"
},
{
"activation": 19.3176212310791,
"uniprot_id": "B0NAQ4"
},
{
"activation": 18.454118728637695,
"uniprot_id": "Q59767"
},
{
"activation": 18... | [
10724,
411,
15016,
6347,
4514,
129,
15234,
769,
4193,
15911
] | 0.207627 |
89 | Summary: Long, compositionally biased intrinsically disordered regions (IDRs)—enriched in Lys/Arg, Glu/Asp, Ser/Thr and often Pro—that occur as extended regulatory tails or linkers (frequently C‑terminal) flanking folded catalytic domains; these segments commonly harbor clusters of PTM sites (e.g., phosphorylation or O... | Long, compositionally biased intrinsically disordered regions (IDRs)—enriched in Lys/Arg, Glu/Asp, Ser/Thr and often Pro—that occur as extended regulatory tails or linkers (frequently C‑terminal) flanking folded catalytic domains; these segments commonly harbor clusters of PTM sites (e.g., phosphorylation or O‑glycosyl... | Strong regional activation over long, contiguous low‑complexity stretches with low predicted structure, typically beginning immediately after a folded domain and extending to the terminus (often C‑terminal); peaks on polyacidic/basic runs, Ser/Thr‑rich and Pro‑rich tracts; minimal activation on the structured catalytic... | Disorder | - RNA decapping enzymes (Nudix/DCP2-like): activation on the disordered C‑terminal regulatory tail following the Nudix domain, rich in acidic/basic residues and phosphoserines (as in the m7GpppN-mRNA hydrolase example).
- Other RNA metabolism/decay factors (decapping cofactors, exosome adaptors): activation on extended... | 9,124,106 | 3.126645 | 13.460938 | [
{
"activation": 13.4609375,
"uniref_id": "A0A806KGH4"
},
{
"activation": 13.265625,
"uniref_id": "UPI002D7F1075"
},
{
"activation": 13.1796875,
"uniref_id": "UPI002609C6FB"
},
{
"activation": 13.171875,
"uniref_id": "UPI0022E6C9CA"
},
{
"activation": 13.140625,
... | [
{
"activation": 11.77958869934082,
"uniprot_id": "P32606"
},
{
"activation": 11.741817474365234,
"uniprot_id": "O74832"
},
{
"activation": 9.246404647827148,
"uniprot_id": "Q8SZZ8"
},
{
"activation": 8.434033393859863,
"uniprot_id": "Q659A1"
},
{
"activation": 8.2... | [
13416,
8362,
8555,
2140,
15308,
13099,
6651,
623,
8407,
7516
] | 0.387015 |
90 | Summary: Non-catalytic, peripheral interaction segments enriched in hydrophobic/basic residues—most often amphipathic alpha-helices (including coiled-coil–like helices) and adjacent flexible loops—found outside catalytic cores, such as C-terminal accessory regions of reverse transcriptase/nucleotidyltransferase-family ... | Non-catalytic, peripheral interaction segments enriched in hydrophobic/basic residues—most often amphipathic alpha-helices (including coiled-coil–like helices) and adjacent flexible loops—found outside catalytic cores, such as C-terminal accessory regions of reverse transcriptase/nucleotidyltransferase-family enzymes a... | Regional activation on contiguous stretches. In enzyme/RT-related proteins, long, well-structured helical tracts occur in mid-to-late sequence positions beyond the annotated RT core; in secreted/receptor/viral proteins, shorter helical or loop patches appear in extracellular domains (coiled-coils or CRD/loop segments),... | Interaction site | - Telomerase reverse transcriptase: strong continuous activation on the C-terminal extension downstream of the RT domain, composed of multiple alpha-helices.
- Group II intron RT/maturase: activation on two C-terminal helices within the intron maturase domain; RT core remains inactive.
- Noncanonical poly(A) polymerase... | 8,800,236 | 3.162787 | 21.71875 | [
{
"activation": 21.71875,
"uniref_id": "A0A3N4KE72"
},
{
"activation": 21.625,
"uniref_id": "A0A317SFP6"
},
{
"activation": 21.28125,
"uniref_id": "A0A1R1WZF7"
},
{
"activation": 21.265625,
"uniref_id": "A0A1Y2C6I9"
},
{
"activation": 21.25,
"uniref_id": "A0A1... | [
{
"activation": 16.946104049682617,
"uniprot_id": "P21329"
},
{
"activation": 15.372613906860352,
"uniprot_id": "O00370"
},
{
"activation": 15.012147903442383,
"uniprot_id": "P16423"
},
{
"activation": 14.496796607971191,
"uniprot_id": "P03239"
},
{
"activation": ... | [
4260,
7795,
5348,
4872,
9099,
16375,
6501,
7378,
16244,
16183
] | 0.219437 |
91 | Summary: Short conserved segment of the HATPase_c (Bergerat-fold) catalytic/ATP‑binding subdomain of GHKL ATPases, most strongly the CA domain of two‑component sensor histidine kinases; the feature keys on an F‑box/ATP‑lid–centered motif (hydrophobic‑F‑E‑[R/K/P/D]‑F) shared across HKs and weakly present in other GHKL f... | Short conserved segment of the HATPase_c (Bergerat-fold) catalytic/ATP‑binding subdomain of GHKL ATPases, most strongly the CA domain of two‑component sensor histidine kinases; the feature keys on an F‑box/ATP‑lid–centered motif (hydrophobic‑F‑E‑[R/K/P/D]‑F) shared across HKs and weakly present in other GHKL families | Regional activation on short 10–16 aa stretches in the C‑terminal half of histidine kinases (typically positions ~300–900), with sharp residue‑level peaks at a hydrophobic‑F‑E‑[R/K/P/D]‑F motif; usually one segment per protein, occasionally multiple in large hybrid HKs; weaker matches in non‑HK GHKL ATPases | Ligand-binding site | - Canonical bacterial two‑component sensor histidine kinases (EnvZ/PhoQ/NarX/NtrB/CheA families): activation on the CA (HATPase_c) F‑box/ATP‑lid region.
- Metal/ion and osmolarity sensors (e.g., Mg2+‑sensing PhoQ, K+ or osmolarity systems like KdpD/EnvZ): activation on the same CA subdomain motif.
- Nitrate/nitrite and... | 5,920,116 | 3.559209 | 29.921875 | [
{
"activation": 29.921875,
"uniref_id": "UPI002028B694"
},
{
"activation": 29.875,
"uniref_id": "A0A925JCR7"
},
{
"activation": 29.875,
"uniref_id": "A0A936KK52"
},
{
"activation": 29.8125,
"uniref_id": "UPI00286A5C5D"
},
{
"activation": 29.78125,
"uniref_id":... | [
{
"activation": 28.854530334472656,
"uniprot_id": "Q2T0V9"
},
{
"activation": 28.428659439086914,
"uniprot_id": "O34206"
},
{
"activation": 28.35117530822754,
"uniprot_id": "P08401"
},
{
"activation": 28.334552764892578,
"uniprot_id": "P52101"
},
{
"activation": 2... | [
7701,
5490,
6588,
14355,
7693,
515,
2246,
13180,
9273,
12074
] | 0.209053 |
92 | Summary: Activation segment of protein kinase catalytic domains (the DFG…APE region), i.e., the activation loop and its flanking conserved motifs; the feature keys on the DFG Phe–Gly (FG) signature, activation‑loop residues (often including a Tyr or a Thr–X–Tyr pair), and the APE/p+1 loop with prolines and acidic resid... | Activation segment of protein kinase catalytic domains (the DFG…APE region), i.e., the activation loop and its flanking conserved motifs; the feature keys on the DFG Phe–Gly (FG) signature, activation‑loop residues (often including a Tyr or a Thr–X–Tyr pair), and the APE/p+1 loop with prolines and acidic residues, and ... | Regional activation within the kinase catalytic domain: a tight cluster of ~9–15 residues with strong peaks at the DFG FG/GF motif, activation‑loop phospho‑acceptors (Thr/Tyr), and the APE Pro/Glu region (often including P–x–P or PP); occasionally extends to the adjacent catalytic‑loop/HRD vicinity | Sequence motif | - Bacterial eukaryotic‑like Ser/Thr kinases (Pkn-family ELKs; e.g., Mycobacterium/Myxococcus): activation on the DFG motif and the DFG→activation‑loop→APE segment.
- MAP kinases (TxY activation motif): activation on DFG, the Thr–X–Tyr pair in the activation loop, and the APE/p+1 loop.
- CDKs and AGC-family kinases: act... | 22,822,147 | 2.209834 | 24.0625 | [
{
"activation": 24.0625,
"uniref_id": "A0A7Y6PN19"
},
{
"activation": 23.859375,
"uniref_id": "A0A7V5RPQ7"
},
{
"activation": 23.859375,
"uniref_id": "A0A538QZZ8"
},
{
"activation": 23.78125,
"uniref_id": "A0A956BUT9"
},
{
"activation": 23.75,
"uniref_id": "UP... | [
{
"activation": 21.721885681152344,
"uniprot_id": "P54737"
},
{
"activation": 21.688817977905273,
"uniprot_id": "Q9FAB3"
},
{
"activation": 21.036623001098633,
"uniprot_id": "Q9RRH3"
},
{
"activation": 20.80927848815918,
"uniprot_id": "P33973"
},
{
"activation": 2... | [
13470,
15288,
598,
2935,
3351,
379,
2057,
6300,
6851,
6755
] | 0.645826 |
93 | Summary: Long, amphipathic coiled‑coil (heptad‑repeat) alpha‑helical rods that function as peripheral stalks/stators and assembly scaffolds in large protein complexes and cytoskeletal systems
Activation pattern: Regional/domain‑level activation across extended helices with periodic residue‑level peaks consistent with t... | Long, amphipathic coiled‑coil (heptad‑repeat) alpha‑helical rods that function as peripheral stalks/stators and assembly scaffolds in large protein complexes and cytoskeletal systems | Regional/domain‑level activation across extended helices with periodic residue‑level peaks consistent with the coiled‑coil heptad register (hydrophobic a/d positions and supportive charged e/g positions); enriched for L/I/V/A and K/E/R/Q, depleted in Gly/Pro; often spans a single long helix or contiguous coiled‑coil se... | Structural motif | - ATP synthase peripheral stalk subunits (F‑type b/b′; V/A‑type E/G): activation along the long dimeric coiled‑coil rods that form the stator.
- Type III secretion/peripheral stalk components: activation on their helical coiled‑coil stator/stalk regions.
- Cell division scaffolds DivIVA/Wag31: strong activation across ... | 19,513,158 | 2.366476 | 29.65625 | [
{
"activation": 29.65625,
"uniref_id": "X1UX36"
},
{
"activation": 29.546875,
"uniref_id": "A0A9E6F9B2"
},
{
"activation": 29.390625,
"uniref_id": "A0A1F7WX57"
},
{
"activation": 29.25,
"uniref_id": "K2CSD4"
},
{
"activation": 29.25,
"uniref_id": "A0A1F7YLI8"
... | [
{
"activation": 27.142436981201172,
"uniprot_id": "O31774"
},
{
"activation": 26.671964645385742,
"uniprot_id": "Q15SF2"
},
{
"activation": 26.528392791748047,
"uniprot_id": "Q3B400"
},
{
"activation": 26.244543075561523,
"uniprot_id": "Q21ZA0"
},
{
"activation": ... | [
7016,
15115,
6375,
13430,
9728,
8975,
5712,
9745,
12950,
11760
] | 0.296313 |
94 | Summary: Short glycine/serine/proline–enriched coil/turn segments at secondary‑structure junctions (beta‑turns and helix‑capping loops), typically the small loops connecting strands or marking coil→helix/coil→strand transitions; a generic structural loop motif that recurs across diverse enzymes and repeat scaffolds and... | Short glycine/serine/proline–enriched coil/turn segments at secondary‑structure junctions (beta‑turns and helix‑capping loops), typically the small loops connecting strands or marking coil→helix/coil→strand transitions; a generic structural loop motif that recurs across diverse enzymes and repeat scaffolds and can lie ... | Residue-level peaks clustered over 3–8 residues, with maxima often on Gly/Ser/Pro; occurs in DSSP “C” coils or at boundaries between coils and helices/strands; frequently repeats periodically within repeat domains; commonly in flexible/low‑pLDDT regions but also in well‑defined loops; multiple short clusters per protei... | Structural motif | • DNA ligases: short helix/turn segment within the C‑terminal ligase domain (coil flanking a short helix).
• Integrin alpha FG‑GAP beta‑propellers: discrete G/P‑centered turns and coil→helix transitions within FG‑GAP repeats.
• Kelch‑repeat beta‑propeller scaffolds: inter‑strand connector loops repeated across kelch bl... | 87,174,202 | 0.869657 | 13.007813 | [
{
"activation": 13.0078125,
"uniref_id": "UPI003014B4E9"
},
{
"activation": 12.8671875,
"uniref_id": "UPI002729AC81"
},
{
"activation": 12.8125,
"uniref_id": "UPI0026003D0D"
},
{
"activation": 12.7734375,
"uniref_id": "UPI00307E4EA1"
},
{
"activation": 12.75,
... | [
{
"activation": 9.600691795349121,
"uniprot_id": "A0R5T2"
},
{
"activation": 9.502592086791992,
"uniprot_id": "A2QVZ0"
},
{
"activation": 9.370346069335938,
"uniprot_id": "H6LC30"
},
{
"activation": 9.361666679382324,
"uniprot_id": "Q9ZEP5"
},
{
"activation": 9.33... | [
8071,
5069,
14101,
951,
3781,
14585,
14093,
7533,
7534,
11958
] | 0.125844 |
95 | Summary: N-terminal subdomain of lithium-sensitive/metallo-dependent phosphomonoesterases centered on the IMPase/IPPase/PAP (SuhB/IMPL, BPNT) family, characterized by acidic Ser/Thr- and Gly-rich loop/β-strand/α-helix elements that precede the catalytic pocket and can include an N-terminal Mg2+-ligating Asp/Glu; also c... | N-terminal subdomain of lithium-sensitive/metallo-dependent phosphomonoesterases centered on the IMPase/IPPase/PAP (SuhB/IMPL, BPNT) family, characterized by acidic Ser/Thr- and Gly-rich loop/β-strand/α-helix elements that precede the catalytic pocket and can include an N-terminal Mg2+-ligating Asp/Glu; also captured i... | Regional activation spanning the N-terminal half of the phosphatase domain, with a sharp onset at the domain boundary (immediately after transit/signal peptides when present), broad elevated signal across early loops/strands/helices, and localized peaks in D/T-rich or glycine-rich loops (occasionally at an N-terminal m... | Domain | - Inositol polyphosphate 1-phosphatase (IMPase family): activation over the N-terminal lobe/loops; includes an N-terminal Mg2+-binding acidic residue in some cases; no activation in the C-terminal substrate pocket.
- Plant chloroplastic IMPL2 (inositol monophosphatase-like phosphatase): activation begins at the start o... | 4,242,189 | 3.892486 | 28.453125 | [
{
"activation": 28.453125,
"uniref_id": "A0AAJ8LJ67"
},
{
"activation": 28.390625,
"uniref_id": "A0A9W8Y595"
},
{
"activation": 28.359375,
"uniref_id": "A0A524N8G4"
},
{
"activation": 28.34375,
"uniref_id": "A0A8T5W1Y7"
},
{
"activation": 28.1875,
"uniref_id":... | [
{
"activation": 26.621368408203125,
"uniprot_id": "P9WKI9"
},
{
"activation": 26.321992874145508,
"uniprot_id": "Q05533"
},
{
"activation": 25.70586395263672,
"uniprot_id": "Q2KJ53"
},
{
"activation": 25.26185417175293,
"uniprot_id": "O67791"
},
{
"activation": 25... | [
11263,
10115,
5024,
8391,
6939,
8580,
681,
5129,
4507,
13811
] | 0.141651 |
96 | Summary: Long, compositionally biased, phosphorylation-prone intrinsically disordered terminal regions that serve as flexible interaction/regulatory segments in eukaryotic chaperone/co-chaperone and adaptor proteins; signal is suppressed within adjacent folded domains (e.g., CS, alpha-crystallin, TPR).
Activation patte... | Long, compositionally biased, phosphorylation-prone intrinsically disordered terminal regions that serve as flexible interaction/regulatory segments in eukaryotic chaperone/co-chaperone and adaptor proteins; signal is suppressed within adjacent folded domains (e.g., CS, alpha-crystallin, TPR). | Regional activation over extended N- or C-terminal stretches (often 50–200+ residues) with low-to-moderate predicted confidence, enriched for acidic/basic and Ser/Pro/Gly residues and sometimes transient/coiled-coil propensity; minimal activation within well-structured globular domains; occasional local peaks around an... | Disorder | - NudC/CS-domain proteins (nuclear movement/chaperone adaptors): strong activation on the N- and C-terminal disordered tails harboring phosphosites; suppressed over the central CS domain.
- Small heat shock proteins (alpha-crystallin family): activation across the long, low-complexity N-terminal region; little to no ac... | 6,501,855 | 3.465478 | 21 | [
{
"activation": 21,
"uniref_id": "UPI0005F55DB7"
},
{
"activation": 20.96875,
"uniref_id": "A0A3P7BTS5"
},
{
"activation": 20.953125,
"uniref_id": "UPI00351F30AE"
},
{
"activation": 20.953125,
"uniref_id": "A0A6J1QI88"
},
{
"activation": 20.953125,
"uniref_id"... | [
{
"activation": 19.840023040771484,
"uniprot_id": "Q5VJS5"
},
{
"activation": 19.672313690185547,
"uniprot_id": "Q5FVL7"
},
{
"activation": 18.047168731689453,
"uniprot_id": "O94966"
},
{
"activation": 17.189611434936523,
"uniprot_id": "P17624"
},
{
"activation": ... | [
5843,
7582,
3042,
7894,
15630,
13160,
16206,
6443,
15872,
12117
] | 0.237137 |
97 | Summary: Heptad‑repeat amphipathic α‑helical coiled‑coil interaction surface used for oligomerization/assembly (residues lying on one helical face, enriched for L/I/V/A with interspersed charged K/E/R/Q, glycine/proline disfavored)
Activation pattern: Residue‑level peaks recurring every ~7 residues within a single cont... | Heptad‑repeat amphipathic α‑helical coiled‑coil interaction surface used for oligomerization/assembly (residues lying on one helical face, enriched for L/I/V/A with interspersed charged K/E/R/Q, glycine/proline disfavored) | Residue‑level peaks recurring every ~7 residues within a single contiguous α‑helical region (often near the N‑terminus); peaks align along one helical face consistent with coiled‑coil a/d (and supportive e/g) positions; little to no activation in β‑strands or disordered segments; typically 5–15 residues per protein | Structural motif | - Prefoldin subunits: periodic residues along the long coiled‑coil “tentacle” helices (heptad pattern on one face)
- ESCRT‑III/CHMP proteins: N‑terminal/central helical hairpin interface used in polymerization, selecting the coiled‑coil/amphipathic interaction face
- ATP synthase F1 ε/δ subunits: C‑terminal/peripheral ... | 48,135,522 | 1.463545 | 18.359375 | [
{
"activation": 18.359375,
"uniref_id": "UPI002916F2F4"
},
{
"activation": 18.1875,
"uniref_id": "UPI001EDE94E4"
},
{
"activation": 18.125,
"uniref_id": "UPI0028E78247"
},
{
"activation": 18.125,
"uniref_id": "A0A9X2HG65"
},
{
"activation": 18.03125,
"uniref_i... | [
{
"activation": 13.751052856445312,
"uniprot_id": "P59074"
},
{
"activation": 13.650548934936523,
"uniprot_id": "Q72V44"
},
{
"activation": 13.050939559936523,
"uniprot_id": "Q12513"
},
{
"activation": 13.010936737060547,
"uniprot_id": "Q89462"
},
{
"activation": ... | [
7283,
9171,
8688,
4400,
5141,
8849,
12987,
622,
2671,
12555
] | 0.446314 |
98 | Summary: Alpha-helical scaffold repeats of the Armadillo/HEAT/TPR superfamily, with strongest signal on the repeating helix–turn–helix units; the feature also captures some long coiled-coil scaffold/adaptor proteins that present a similar repetitive helical geometry in eukaryotes.
Activation pattern: Domain-level activ... | Alpha-helical scaffold repeats of the Armadillo/HEAT/TPR superfamily, with strongest signal on the repeating helix–turn–helix units; the feature also captures some long coiled-coil scaffold/adaptor proteins that present a similar repetitive helical geometry in eukaryotes. | Domain-level activation spanning long arrays of helical repeats with regularly spaced residue-level peaks in the coil/turn connectors between helices (and adjacent helical residues); minimal activation in non-solenoid enzymatic domains and signal/transit peptides. In coiled-coil scaffolds, activation can extend along t... | Domain | - SARM1 (innate immunity NAD+ hydrolase): activation confined to the N‑terminal Armadillo repeat domain, peaking in loop/turns; absent in SAM and TIR catalytic domains and in the transit peptide.
- Hsp70 nucleotide-exchange factor Fes1: activation along Armadillo repeats, strongest in coil/turn connectors (with occasio... | 13,048,796 | 2.768869 | 23.71875 | [
{
"activation": 23.71875,
"uniref_id": "A0A7K4IU23"
},
{
"activation": 23.375,
"uniref_id": "A0A0R3T4I7"
},
{
"activation": 23.3125,
"uniref_id": "A0A7K5H8J7"
},
{
"activation": 23.21875,
"uniref_id": "A0A0R3SIA5"
},
{
"activation": 23.21875,
"uniref_id": "A0A... | [
{
"activation": 22.374755859375,
"uniprot_id": "Q10435"
},
{
"activation": 22.153709411621094,
"uniprot_id": "Q6WWW4"
},
{
"activation": 22.0976619720459,
"uniprot_id": "Q14669"
},
{
"activation": 22.01848602294922,
"uniprot_id": "P33202"
},
{
"activation": 21.944... | [
3731,
7284,
16246,
12951,
16036,
14186,
977,
12704,
14583,
1582
] | 0.456808 |
99 | Summary: Glycine‑centered signal—strongest on collagen‑like Gly‑X‑Y repeats and Gly/Ser/Thr‑rich flexible linkers/tails common in secreted/extracellular and small regulatory proteins—and also on isolated glycine‑dependent motifs in otherwise structured proteins; includes the glycine‑rich inter‑domain linker of ECF/alte... | Glycine‑centered signal—strongest on collagen‑like Gly‑X‑Y repeats and Gly/Ser/Thr‑rich flexible linkers/tails common in secreted/extracellular and small regulatory proteins—and also on isolated glycine‑dependent motifs in otherwise structured proteins; includes the glycine‑rich inter‑domain linker of ECF/alternative s... | Residue‑level peaks on glycine (secondarily serine/threonine) that either occur as isolated hotspots (e.g., conserved glycine(s) in flexible loops/motifs such as the Walker A/P‑loop) or expand into regional activation across repetitive Gly‑X‑Y tracts or other low‑complexity stretches; often includes the first few N‑ter... | Compositional bias | - Fibrillar collagens and collagen‑like proteins: strong, periodic activation across Gly‑X‑Y triple‑helix repeats (peaks on every third Gly)
- Bacterial ECF (alternative) sigma factors: activation on the glycine‑rich flexible linker connecting sigma2 and sigma4 domains (mid/C‑terminal region)
- Secreted micropeptides, ... | 90,610,173 | 0.830999 | 12.234375 | [
{
"activation": 12.234375,
"uniref_id": "A0A3L6FW01"
},
{
"activation": 11.59375,
"uniref_id": "P82436"
},
{
"activation": 11.171875,
"uniref_id": "A0A2P4SBJ8"
},
{
"activation": 10.96875,
"uniref_id": "A0A6V7M7Z1"
},
{
"activation": 10.9140625,
"uniref_id": "... | [
{
"activation": 6.611855983734131,
"uniprot_id": "Q02940"
},
{
"activation": 6.367056369781494,
"uniprot_id": "A0A1B0GTK4"
},
{
"activation": 6.2474212646484375,
"uniprot_id": "P03086"
},
{
"activation": 6.209904193878174,
"uniprot_id": "P9WGH5"
},
{
"activation":... | [
8375,
10298,
10582,
11830,
6352,
11192,
15679,
10709,
7547,
6757
] | 0.229952 |
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