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# APED Pipeline Configuration
# For reproducibility of results
# Dataset Parameters
dataset:
name: "APED-Thermophile-Module"
version: "1.0.0"
# Data Sources
sources:
alphafold_db: "https://alphafold.ebi.ac.uk"
uniprot: "https://www.uniprot.org"
# Organism Taxonomy IDs (UniProt)
organisms:
- name: "Thermus thermophilus"
taxonomy_id: 274
- name: "Thermococcus kodakarensis"
taxonomy_id: 69014
- name: "Sulfolobus solfataricus"
taxonomy_id: 2287
- name: "Pyrococcus furiosus"
taxonomy_id: 2261
# Feature Extraction Parameters
features:
structural:
- mean_plddt
- helix_fraction
- sheet_fraction
- coil_fraction
sequence:
- sequence_length
- molecular_weight
- isoelectric_point
- instability_index
- aromaticity
- hydrophobicity
- charge
# RFdiffusion Parameters
rfdiffusion:
colab_notebook: "https://colab.research.google.com/github/sokrypton/ColabDesign/blob/main/rf/examples/diffusion.ipynb"
parameters:
contigs: "A1-50/50-100"
iterations: 50
num_designs: 5
symmetry: "none"
use_beta_model: false
# ProteinMPNN Parameters
proteinmpnn:
sequences_per_backbone: 8
sampling_temperature: 0.1
# Validation Thresholds
validation:
min_plddt: 90.0
max_rmsd: 1.0
min_ptm: 0.7
# Output
output:
huggingface_repo: "electricsheepafrica/APED-African-Protein-Engineering-Dataset"
formats:
- parquet
- fasta
- csv