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# NCBI RefSeq Protein Shard Index
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Use the original `sequences/.../shard-*.fasta.zst` files for complete FASTA records. Use the default Parquet table for Dataset Viewer previews, source discovery, file sizes, record counts, and download patterns.
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## Splits
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## Preparation
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The normalization script used to create the Parquet files is included at `scripts/prepare_ncbi_dataset.py`.
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# NCBI RefSeq Protein Shard Index
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The RefSeq protein database is the protein subset of NCBI's broader Reference Sequence collection, a curated non-redundant set of genomic, transcript, and protein sequences spanning the tree of life. Protein records are produced through a combination of expert curation, the NCBI Eukaryotic and Prokaryotic Genome Annotation pipelines, and propagation from collaborating resources, and are cross-linked to their source genome and transcript records. RefSeq protein accessions use prefixed identifiers (NP_ for curated, XP_ for predicted, WP_ for non-redundant prokaryotic proteins, YP_ for organelle and viral proteins) so that downstream tools can distinguish curation status at a glance.
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## Splits
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## Preparation
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The normalization script used to create the Parquet files is included at `scripts/prepare_ncbi_dataset.py`.
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# Citation
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```
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@article{goldfarb2025refseq,
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title = {{NCBI RefSeq}: reference sequence standards through 25 years of curation and annotation},
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author = {Goldfarb, Tamara and Kodali, Vamsi K. and Pujar, Shashikant and Brover, Vyacheslav and Robbertse, Barbara and Farrell, Catherine M. and Oh, Dong-Ha and Astashyn, Alexander and Ermolaeva, Olga and Haddad, Diana and Hlavina, Wratko and Hoffman, Jinna and Jackson, John D. and Joardar, Vinita S. and Kristensen, David and Masterson, Patrick and McGarvey, Kelly M. and McVeigh, Richard and Mozes, Eyal and Murphy, Michael R. and Schafer, Susan S. and Souvorov, Alexander and Spurrier, Brett and Strope, Pooja K. and Sun, Hanzhen and Vatsan, Anjana R. and Wallin, Craig and Webb, David and Brister, J. Rodney and Hatcher, Eneida and Kimchi, Avi and Klimke, William and Marchler-Bauer, Aron and Pruitt, Kim D. and Thibaud-Nissen, Fran{\c{c}}oise and Murphy, Terence D.},
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journal = {Nucleic Acids Research},
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volume = {53},
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number = {D1},
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pages = {D243--D257},
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year = {2025},
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publisher = {Oxford University Press},
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doi = {10.1093/nar/gkae1038}
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}
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```
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